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SH2D3C Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS648270

Eph receptor-binding protein which may be a positive regulator of TCR signaling. Binding to BCAR1 is required to induce membrane ruffling and promote EGF-dependent cell migration. More details

$880 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SH2D3C, Monoclonal Antibody
Full Product Name
SH2D3C (SH2 Domain-Containing Protein 3C, Novel SH2-Containing Protein 3, NSP3, UNQ272/PRO309/PRO34088)
Product Gene Name
anti-SH2D3C antibody
Product Synonym Names
Anti -SH2D3C (SH2 Domain-Containing Protein 3C, Novel SH2-Containing Protein 3, NSP3, UNQ272/PRO309/PRO34088)
Immunogen Sequence
MTAVGRRCPA LGSRGAAGEP EAGSDYVKFS KEKYILDSSP EKLHKELEEE LKLSSTDLRS HAWYHGRIPR EVSETLVQRN GDFLIRDSLT SLGDYVLTCR WRNQALHFKI
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3B2
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human SH2D3C.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
94,411 Da
Chromosome Location
Chromosome: 9; NC_000009.11 (130500596..130541048, complement). Location: 9q34.11
OMIM
604722
3D Structure
ModBase 3D Structure for Q8N5H7
Immunogen
Partial recombinant corresponding to aa1-110 from SH2D3C (NP_005480) with GST tag. MW of the GST tag alone is 26kD.

Product Categories/Family for anti-SH2D3C antibody

Antibodies; Abs to Proteins
Applications Tested/Suitable for anti-SH2D3C antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-SH2D3C antibody
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml

Western Blot (WB) of anti-SH2D3C antibody

Western Blot detection against Immunogen (37.84kD).
Mouse Anti-SH2D3C Monoclonal Antibody – Cat# MBS648270

Western Blot (WB) of anti-SH2D3C antibody

SH2D3C monoclonal antibody Western Blot analysis of SH2D3C expression in HeLa NE
Mouse Anti-SH2D3C Monoclonal Antibody, Western Blot (WB) – Cat# MBS648270

Immunohistochemistry (IHC) of anti-SH2D3C antibody

Immunoperoxidase of monoclonal antibody to SH2D3C on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3ug/ml]
Mouse Anti-SH2D3C Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS648270

Testing Data of anti-SH2D3C antibody

Detection limit for recombinant GST tagged SH2D3C is ~0.1ng/ml as a capture antibody.
Mouse Anti-SH2D3C Monoclonal Antibody, Testing Data – Cat# MBS648270

Other Notes

Small volumes of anti-SH2D3C antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SH2D3C purchase

MBS648270 is an antibody targeting SH2D3C. Also known as SH2D3C (SH2 Domain-Containing Protein 3C, Novel SH2-Containing Protein 3, NSP3, UNQ272/PRO309/PRO34088). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for SH2D3C. It may not necessarily be applicable to this product.

NCBI GI #
356640213
NCBI GeneID
10044
NCBI Accession #
NP_001239263.1
UniProt Primary Accession #
Q8N5H7
UniProt Secondary Accession #
Q5HYE5; Q5JU31; Q6UY42; Q8N6X3; Q9Y2X5; A8K5S8; E9PG48
UniProt Related Accession #
Q8N5H7
GenBank Nucleotide #
NM_001252334.1
NCBI Official Full Name
SH2 domain-containing protein 3C isoform f
NCBI Official Synonym Full Names
SH2 domain containing 3C
NCBI Official Symbol
SH2D3C
NCBI Official Synonym Symbols
CHAT; NSP3; SHEP1; PRO34088
UniProt Protein Name
SH2 domain-containing protein 3C
UniProt Synonym Protein Names
Novel SH2-containing protein 3; SH2 domain-containing Eph receptor-binding protein 1
Protein Family
SH2 domain-containing protein
UniProt Gene Name
SH2D3C
UniProt Synonym Gene Names
NSP3; SHEP1
UniProt Entry Name
SH2D3_HUMAN
NCBI Protein Information
SH2 domain-containing protein 3C; novel SH2-containing protein 3; SH2 domain-containing Eph receptor-binding protein 1

NCBI Summary for SH2D3C

This gene encodes an adaptor protein and member of a cytoplasmic protein family involved in cell migration. The encoded protein contains a putative Src homology 2 (SH2) domain and guanine nucleotide exchange factor-like domain which allows this signaling protein to form a complex with scaffolding protein Crk-associated substrate. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
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UniProt Comments for SH2D3C

Function: Eph receptor-binding protein which may be a positive regulator of TCR signaling. Binding to BCAR1 is required to induce membrane ruffling and promote EGF-dependent cell migration

By similarity.

Subunit structure: Interacts with PTK2B. Interacts with NEDD9/HEF1

By similarity. Interacts (via C-terminus) with BCAR1/CAS.

Subcellular location: Cytoplasm

By similarity. Membrane; Peripheral membrane protein

By similarity. Note: Associated with the membrane when EGF-stimulated. Found at ruffling membranes

By similarity.

Tissue specificity: Ubiquitously expressed. Ref.1

Domain: The C-terminal Cdc25-homology/Ras-GEF domain adopts a closed conformation rendering it incapable of carrying out canonical exchange factor function, this closed conformation is required for interaction with BCAR1.

Sequence similarities: Contains 1 Ras-GEF domain.Contains 1 SH2 domain.

Sequence caution: The sequence AAQ88456.1 differs from that shown. Reason: Erroneous initiation.
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Research Articles on SH2D3C

1. The structure of the NSP3-p130Cas complex reveals that this closed conformation is instrumental for interaction of NSP proteins with a focal adhesion-targeting domain present in Cas proteins.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SH2D3C antibody

 Reference Product  PubMed Publications
 SRC antibody  >7 publications with SH2D3C and SRC
 BCAR1 antibody  >5 publications with SH2D3C and BCAR1
 EPHB2 antibody  >2 publications with SH2D3C and EPHB2
 NEDD9 antibody  >1 publications with SH2D3C and NEDD9

Pathways associated with anti-SH2D3C antibody

 Products by Pathway  Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram

Diseases associated with anti-SH2D3C antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >4 publications with SH2D3C and Neoplasms
 Skin Diseases Antibodies  >3 publications with SH2D3C and Skin Diseases
 Disease Models, Animal Antibodies  >1 publications with SH2D3C and Disease Models, Animal

Organs/Tissues associated with anti-SH2D3C antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >4 publications with SH2D3C and Nerve
 Blood Antibodies  >4 publications with SH2D3C and Blood
 Brain Antibodies  >4 publications with SH2D3C and Brain
 Embryonic Tissue Antibodies  >2 publications with SH2D3C and Embryonic Tissue
 Spleen Antibodies  >2 publications with SH2D3C and Spleen
 Stomach Antibodies  >1 publications with SH2D3C and Stomach
 Vascular Antibodies  >1 publications with SH2D3C and Vascular
 Muscle Antibodies  >1 publications with SH2D3C and Muscle
 Thymus Antibodies  >1 publications with SH2D3C and Thymus
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