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FASN Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS648398

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
FASN, Monoclonal Antibody
Full Product Name
FASN, ID (FASN, FAS, Fatty acid synthase, [Acyl-carrier-protein] S-acetyltransferase, [Acyl-carrier-protein] S-malonyltransferase, 3-oxoacyl-[acyl-carrier-protein] synthase, 3-oxoacyl-[acyl-carrier-protein] reductase, 3-hydroxypalmitoyl-[acyl-carrier-prot
Product Gene Name
anti-FASN antibody
Product Synonym Names
Anti -FASN, ID (FASN, FAS, Fatty acid synthase, [Acyl-carrier-protein] S-acetyltransferase, [Acyl-carrier-protein] S-malonyltransferase, 3-oxoacyl-[acyl-carrier-protein] synthase, 3-oxoacyl-[acyl-carrier-protein] reductase, 3-hydroxypalmitoyl-[acyl-carrier-prot
Clonality
Monoclonal
Isotype
IgM
Clone Number
497CT15.2.5
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid, crude ascites with 0.09% sodium azide.
Purity/Purification
Ascites
Ascites
Specificity
Human, mouse
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
273,427 Da
Chromosome Location
Chromosome: 17; NC_000017.10 (80036214..80056106, complement). Location: 17q25
OMIM
600212
3D Structure
ModBase 3D Structure for P49327
Immunogen
FASN Mab is generated from mouses immunized with a KLH conjugated synthetic peptide selected from the center region of human FASN.

Product Categories/Family for anti-FASN antibody

Antibodies; Abs to Enzymes; Acetyltransferase
Applications Tested/Suitable for anti-FASN antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-FASN antibody
Suitable for use in Western Blot, ELISA.
Dilution: ELISA: 1:1,000
Western Blot: 1:100~1000

Testing Data of anti-FASN antibody

Fluorescent confocal image of HepG2 cells stained with FASN (Center) antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM2067a FASN primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the FASN immunosignal to the cytoplasm, supported by Human Protein Atlas Data .
Mouse Anti-FASN Monoclonal Antibody – Cat# MBS648398

Western Blot (WB) of anti-FASN antibody

FASN Antibody (Center) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the FASN antibody detected the FASN protein (arrow).
Mouse Anti-FASN Monoclonal Antibody, Western Blot (WB) – Cat# MBS648398

Other Notes

Small volumes of anti-FASN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FASN purchase

MBS648398 is an antibody targeting FASN. Also known as FASN, ID (FASN, FAS, Fatty acid synthase, [Acyl-carrier-protein] S-acetyltransferase, [Acyl-carrier-protein] S-malonyltransferase, 3-oxoacyl-[acyl-carrier-protein] synthase, 3-oxoacyl-[acyl-carrier-protein] reductase, 3-hydroxypalmitoyl-[acyl-carrier-prot. Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for FASN. It may not necessarily be applicable to this product.

NCBI GI #
41872631
NCBI GeneID
2194
NCBI Accession #
NP_004095.4
UniProt Primary Accession #
P49327
UniProt Secondary Accession #
Q13479; Q16702; Q4LE83; Q6P4U5; Q6SS02; Q969R1; Q96C68; Q96IT0
UniProt Related Accession #
P49327
GenBank Nucleotide #
NM_004104.4
NCBI Official Full Name
fatty acid synthase
NCBI Official Synonym Full Names
fatty acid synthase
NCBI Official Symbol
FASN
NCBI Official Synonym Symbols
FAS; OA-519; SDR27X1
UniProt Protein Name
Fatty acid synthase
Protein Family
Fatty acid synthase
UniProt Gene Name
FASN
UniProt Synonym Gene Names
FAS
UniProt Entry Name
FAS_HUMAN
NCBI Protein Information
fatty acid synthase; short chain dehydrogenase/reductase family 27X, member 1

UniProt Comments for FASN

Function: Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.

Catalytic activity: Acetyl-CoA + n malonyl-CoA + 2n NADPH = a long-chain fatty acid + (n+1) CoA + n CO2 + 2n NADP+. Ref.1Acetyl-CoA + [acyl-carrier-protein] = CoA + acetyl-[acyl-carrier-protein]. Ref.1Malonyl-CoA + [acyl-carrier-protein] = CoA + malonyl-[acyl-carrier-protein]. Ref.1Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein]. Ref.1(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH. Ref.1A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H2O. Ref.1Acyl-[acyl-carrier protein] + NADP+ = trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH. Ref.1Oleoyl-[acyl-carrier-protein] + H2O = [acyl-carrier-protein] + oleate. Ref.1

Subunit structure: Homodimer which is arranged in a head to tail fashion.

Subcellular location: Cytoplasm. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Ref.10

Tissue specificity: Ubiquitous. Prominent expression in brain, lung, and liver. Ref.1 Ref.9

Miscellaneous: The relatively low beta-ketoacyl synthase activity may be attributable to the low 4'-phosphopantetheine content of the protein.

Sequence similarities: Contains 1 acyl carrier domain.

Sequence caution: The sequence AAB35516.1 differs from that shown. Reason: Erroneous initiation. The sequence AAC50259.1 differs from that shown. Reason: Several sequencing errors.The sequence BAE06070.1 differs from that shown. Reason: Erroneous initiation.
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Research Articles on FASN

1. FAS and HER2 overexpression are common in esophageal carcinomas

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-FASN antibody

 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Activation Of Gene Expression By SREBP (SREBF) Pathway antibodies  Activation Of Gene Expression By SREBP (SREBF) Pathway Diagram
 ChREBP Activates Metabolic Gene Expression Pathway antibodies  ChREBP Activates Metabolic Gene Expression Pathway Diagram
 Fatty Acid Biosynthesis Pathway antibodies  Fatty Acid Biosynthesis Pathway Diagram
 Fatty Acyl-CoA Biosynthesis Pathway antibodies  Fatty Acyl-CoA Biosynthesis Pathway Diagram
 Fatty Acid Biosynthesis Pathway antibodies  Fatty Acid Biosynthesis Pathway Diagram
 Fatty Acid Biosynthesis Pathway antibodies  Fatty Acid Biosynthesis Pathway Diagram
 Fatty Acid Biosynthesis, Elongation Pathway antibodies  Fatty Acid Biosynthesis, Elongation Pathway Diagram
 Fatty Acid Biosynthesis, Elongation Pathway antibodies  Fatty Acid Biosynthesis, Elongation Pathway Diagram
 Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies  Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram

Diseases associated with anti-FASN antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >161 publications with FASN and Neoplasms
 Fatty Liver Antibodies  >90 publications with FASN and Fatty Liver
 Obesity Antibodies  >77 publications with FASN and Obesity
 Liver Diseases Antibodies  >39 publications with FASN and Liver Diseases
 Breast Neoplasms Antibodies  >38 publications with FASN and Breast Neoplasms
 Insulin Resistance Antibodies  >37 publications with FASN and Insulin Resistance
 Inflammation Antibodies  >29 publications with FASN and Inflammation
 Weight Loss Antibodies  >19 publications with FASN and Weight Loss
 Necrosis Antibodies  >15 publications with FASN and Necrosis
 Hepatitis Antibodies  >14 publications with FASN and Hepatitis

Organs/Tissues associated with anti-FASN antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >107 publications with FASN and Liver
 Adipose Tissue Antibodies  >84 publications with FASN and Adipose Tissue
 Blood Antibodies  >75 publications with FASN and Blood
 Connective Tissue Antibodies  >49 publications with FASN and Connective Tissue
 Muscle Antibodies  >40 publications with FASN and Muscle
 Prostate Antibodies  >36 publications with FASN and Prostate
 Mammary Gland Antibodies  >22 publications with FASN and Mammary Gland
 Lung Antibodies  >14 publications with FASN and Lung
 Brain Antibodies  >12 publications with FASN and Brain
 Vascular Antibodies  >11 publications with FASN and Vascular
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