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CPSF3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS648407

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and… More details

$880 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
CPSF3, Monoclonal Antibody
Full Product Name
CPSF3 (Cleavage and Polyadenylation Specificity Factor Subunit 3, Cleavage and Polyadenylation Specificity Factor 73 kDa Subunit, CPSF 73 kDa Subunit, mRNA 3'-end-processing Endonuclease CPSF-73, CPSF73)
Product Gene Name
anti-CPSF3 antibody
Product Synonym Names
Anti -CPSF3 (Cleavage and Polyadenylation Specificity Factor Subunit 3, Cleavage and Polyadenylation Specificity Factor 73 kDa Subunit, CPSF 73 kDa Subunit, mRNA 3'-end-processing Endonuclease CPSF-73, CPSF73)
Immunogen Sequence
LEVQSNPKIR KGAVQKVSKK LEMHVYSKRL EIMLQDIFGE DCVSVKDDSI LSVTVDGKTA NLNLETRTVE CEEGSEDDES LREMVELAAQ RLYEALTPVH
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
6E6
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human CPSF3. Species Crossreactivity: mouse and rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
77,486 Da
Chromosome Location
Chromosome: 2; NC_000002.11 (9563666..9613239). Location: 2p25.1
OMIM
606029
3D Structure
ModBase 3D Structure for Q9UKF6
Immunogen
Partial recombinant corresponding to aa585-685, from human CPSF3 (NP_057291) with GST tag. MW of the GST tag alone is 26kD.

Product Categories/Family for anti-CPSF3 antibody

Antibodies; Abs to Proteins
Applications Tested/Suitable for anti-CPSF3 antibody
ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-CPSF3 antibody
Suitable for use in Immunofluorescence, ELISA and Western Blot.
Dilution: Immunofluorescence: 10ug/ml

Western Blot (WB) of anti-CPSF3 antibody

Western Blot detection against Immunogen (37.11kD).
Mouse Anti-CPSF3 Monoclonal Antibody – Cat# MBS648407

Western Blot (WB) of anti-CPSF3 antibody

CPSF3 monoclonal antibody. Western Blot analysis of CPSF3 expression in PC-12.
Mouse Anti-CPSF3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS648407

Western Blot (WB) of anti-CPSF3 antibody

CPSF3 monoclonal antibody, Western Blot analysis of CPSF3 expression in Hela NE.
Mouse Anti-CPSF3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS648407

Immunofluorescence (IF) of anti-CPSF3 antibody

Immunofluorescence of monoclonal antibody to CPSF3 on HeLa cell. [antibody concentration 10ug/ml].
Mouse Anti-CPSF3 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS648407

Testing Data of anti-CPSF3 antibody

Detection limit for recombinant GST tagged CPSF3 is ~0.1ng/ml as a capture antibody.
Mouse Anti-CPSF3 Monoclonal Antibody, Testing Data – Cat# MBS648407

Western Blot (WB) of anti-CPSF3 antibody

Western Blot analysis of CPSF3 expression in transfected 293T cell line by CPSF3 monoclonal antibody. Lane 1: CPSF3 transfected lysate (77.5kD).|Lane 2: Non-transfected lysate.
Mouse Anti-CPSF3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS648407

Western Blot (WB) of anti-CPSF3 antibody

CPSF3 monoclonal antibody. Western Blot analysis of CPSF3 expression in NIH/3T3.
Mouse Anti-CPSF3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS648407

Other Notes

Small volumes of anti-CPSF3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CPSF3 purchase

MBS648407 is an antibody targeting CPSF3. Also known as CPSF3 (Cleavage and Polyadenylation Specificity Factor Subunit 3, Cleavage and Polyadenylation Specificity Factor 73 kDa Subunit, CPSF 73 kDa Subunit, mRNA 3'-end-processing Endonuclease CPSF-73, CPSF73). Reactive in Human, Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CPSF3. It may not necessarily be applicable to this product.

NCBI GI #
7706427
NCBI GeneID
51692
NCBI Accession #
NP_057291.1
UniProt Primary Accession #
Q9UKF6
UniProt Secondary Accession #
O14769; Q53RS2; Q96F36
UniProt Related Accession #
Q9UKF6
GenBank Nucleotide #
NM_016207.3
NCBI Official Full Name
cleavage and polyadenylation specificity factor subunit 3
NCBI Official Synonym Full Names
cleavage and polyadenylation specific factor 3, 73kDa
NCBI Official Symbol
CPSF3
NCBI Official Synonym Symbols
CPSF73; CPSF-73
UniProt Protein Name
Cleavage and polyadenylation specificity factor subunit 3
UniProt Synonym Protein Names
Cleavage and polyadenylation specificity factor 73 kDa subunit; CPSF 73 kDa subunit; mRNA 3'-end-processing endonuclease CPSF-73
Protein Family
Cleavage and polyadenylation specificity factor
UniProt Gene Name
CPSF3
UniProt Synonym Gene Names
CPSF73; CPSF 73 kDa subunit
UniProt Entry Name
CPSF3_HUMAN
NCBI Protein Information
cleavage and polyadenylation specificity factor subunit 3; mRNA 3'-end-processing endonuclease CPSF-73

NCBI Summary for CPSF3

This gene encodes a member of the metallo-beta-lactamase family. The encoded protein is a 73kDa subunit of the cleavage and polyadenylation specificity factor and functions as an endonuclease that recognizes the pre-mRNA 3'-cleavage site AAUAAA prior to polyadenylation. It also cleaves after the pre-mRNA sequence ACCCA during histone 3'-end pre-mRNA processing. [provided by RefSeq, Oct 2012]
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UniProt Comments for CPSF3

Function: Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. Ref.6 Ref.7 Ref.9 Ref.15

Cofactor: Binds 2 zinc ions per subunit.

Subunit structure: Component of the cleavage and polyadenylation specificity factor (CPSF) complex, composed of CPSF1, CPSF2, CPSF3, CPSF4 and FIP1L1. Interacts with CPSF2, CSTF2 and SYMPK. Interacts with TUT1; the interaction is direct and mediates the recruitment of the CPSF complex on the 3'UTR of pre-mRNAs. Interacts with WDR33. Ref.6 Ref.9 Ref.11 Ref.12

Subcellular location: Nucleus Ref.7.

Post-translational modification: Sumoylated on Lys-462, Lys-465 and Lys-545, preferentially by SUMO3. Ref.8

Sequence similarities: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF3 subfamily.
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Research Articles on CPSF3

1. the molecular mechanism that recruits the CPSF73 endonuclease to histone pre-mRNAs

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CPSF3 antibody

 Products by Pathway  Pathway Diagram
 Cleavage Of Growing Transcript In The Termination Region Pathway antibodies  Cleavage Of Growing Transcript In The Termination Region Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies  Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
 Processing Of Capped Intronless Pre-mRNA Pathway antibodies  Processing Of Capped Intronless Pre-mRNA Pathway Diagram
 Processing Of Intronless Pre-mRNAs Pathway antibodies  Processing Of Intronless Pre-mRNAs Pathway Diagram
 RNA Polymerase II Transcription Pathway antibodies  RNA Polymerase II Transcription Pathway Diagram
 RNA Polymerase II Transcription Termination Pathway antibodies  RNA Polymerase II Transcription Termination Pathway Diagram
 Transport Of Mature Transcript To Cytoplasm Pathway antibodies  Transport Of Mature Transcript To Cytoplasm Pathway Diagram
 Transport Of Mature MRNA Derived From An Intronless Transcript Pathway antibodies  Transport Of Mature MRNA Derived From An Intronless Transcript Pathway Diagram
 Transport Of Mature MRNAs Derived From Intronless Transcripts Pathway antibodies  Transport Of Mature MRNAs Derived From Intronless Transcripts Pathway Diagram

Organs/Tissues associated with anti-CPSF3 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >1 publications with CPSF3 and Muscle
 Prostate Antibodies  >1 publications with CPSF3 and Prostate
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