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Ketohexokinase (KHK) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6484293

MBS6484293 is an antibody targeting KHK. Also known as Ketohexokinase, NT (KHK, Hepatic Fructokinase) (PE). Reactive in Human, Mouse. Host: Rabbit. More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Ketohexokinase (KHK), Polyclonal Antibody
Full Product Name
Ketohexokinase, NT (KHK, Hepatic Fructokinase) (PE)
Product Gene Name
Product Synonym Names
Ketohexokinase (Fructokinase); EC=2.7.1.3
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein G Affinity Chromatography.
Specificity
Recognizes human KHK. Species Crossreactivity: mouse.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
33kDa
OMIM
229800
3D Structure
ModBase 3D Structure for P50053
Immunogen
Synthetic peptide selected from the N-terminal region of human KHK (KLH).
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-KHK antibody

Applications Tested/Suitable for anti-KHK antibody
Western Blot (WB), FLISA
Application Notes for anti-KHK antibody
WB: 1:100-1:500
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-KHK antibody

Western blot analysis of KHK (arrow) using rabbit polyclonal Ketohexokinase (KHK) Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the KHK gene (Lane 2) (Origene Technologies).
Rabbit Anti-KHK Polyclonal Antibody – Cat# MBS6484293

Western Blot (WB) of anti-KHK antibody

The anti-KHK Pab is used in Western blot to detect KHK in mouse liver tissue lysate (Lane 1) and 293 cell lysate (Lane 2).
Rabbit Anti-KHK Polyclonal Antibody, Western Blot (WB) – Cat# MBS6484293

Product References and Citations for anti-KHK antibody

1. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002). 2. Hayward, B.E., et al., Eur. J. Biochem. 257(1):85-91 (1998). 3. Bonthron, D.T., et al., Hum. Mol. Genet. 3(9):1627-1631 (1994).

Other Notes

Small volumes of anti-KHK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for KHK. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ketohexokinase isoform b
NCBI Official Synonym Full Names
ketohexokinase
NCBI Official Symbol
KHK
UniProt Protein Name
Ketohexokinase
UniProt Synonym Protein Names
Hepatic fructokinase
Protein Family
UniProt Gene Name
KHK
UniProt Entry Name
KHK_HUMAN
NCBI Protein Information
ketohexokinase

NCBI Summary for KHK

This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
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UniProt Comments for KHK

KHK iso2: Defects in KHK are the cause of fructosuria (FRUCT). Benign defect of intermediary metabolism. Belongs to the carbohydrate kinase PfkB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.1.3; Carbohydrate Metabolism - fructose and mannose; Transferase

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: ketohexokinase activity; ATP binding

Biological Process: carbohydrate phosphorylation; carbohydrate metabolic process; pathogenesis; fructose catabolic process

Disease: Fructosuria, Essential
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KHK antibody

 Reference Product  PubMed Publications
 HK1 antibody  >1 publications with KHK and HK1
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