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CYP2A7 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS648499

CYP2A7 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the… More details

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Product Overview

Online Datasheet in PDF
Product Name
CYP2A7, Polyclonal Antibody
Full Product Name
CYP2A7, ID (CYP2A7, Cytochrome P450 2A7, CYPIIA7, Cytochrome P450 IIA4)
Product Gene Name
Product Synonym Names
Anti -CYP2A7, ID (CYP2A7, Cytochrome P450 2A7, CYPIIA7, Cytochrome P450 IIA4)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
56,425 Da
Chromosome Location
Chromosome: 19; NC_000019.9 (41381344..41388657, complement). Location: 19q13.2
OMIM
608054
3D Structure
ModBase 3D Structure for P20853
Immunogen
CYP2A7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 128-156 amino acids from the Central region of human CYP2A7.
Applications Tested/Suitable for anti-CYP2A7 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-CYP2A7 antibody
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-CYP2A7 antibody

CYP2A7 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the CYP2A7 antibody detected the CYP2A7 protein (arrow).
Rabbit Anti-CYP2A7 Polyclonal Antibody – Cat# MBS648499

Immunohistochemistry (IHC) of anti-CYP2A7 antibody

CYP2A7 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CYP2A7 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-CYP2A7 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS648499

Flow Cytometry (FC/FACS) of anti-CYP2A7 antibody

CYP2A7 Antibody (Center) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-CYP2A7 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS648499

Other Notes

Small volumes of anti-CYP2A7 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CYP2A7 purchase

MBS648499 is an antibody targeting CYP2A7. Also known as CYP2A7, ID (CYP2A7, Cytochrome P450 2A7, CYPIIA7, Cytochrome P450 IIA4). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CYP2A7. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
cytochrome P450 2A7 isoform 1
NCBI Official Synonym Full Names
cytochrome P450, family 2, subfamily A, polypeptide 7
NCBI Official Symbol
CYP2A7
NCBI Official Synonym Symbols
CPA7; CPAD; CYP2A; CYPIIA7; P450-IIA4
UniProt Protein Name
Cytochrome P450 2A7
UniProt Synonym Protein Names
CYPIIA7; Cytochrome P450 IIA4
Protein Family
UniProt Gene Name
CYP2A7
UniProt Entry Name
CP2A7_HUMAN
NCBI Protein Information
cytochrome P450 2A7; cytochrome P450IIA4; cytochrome P450 IIA4; cytochrome P450, subfamily IIA (phenobarbital-inducible), polypeptide 7

NCBI Summary for CYP2A7

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum; its substrate has not yet been determined. This gene, which produces two transcript variants, is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. [provided by RefSeq, Jul 2008]
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UniProt Comments for CYP2A7

Function: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activity: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor: Heme group

By similarity.

Subcellular location: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Induction: P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

Sequence similarities: Belongs to the cytochrome P450 family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-CYP2A7 antibody

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