DNAJC2 Antibody – Rabbit Polyclonal

This gene is a member of the M-phase phosphoprotein (MPP) family. The gene encodes a phosphoprotein with a J domain and a Myb DNA-binding domain which localizes to both the nucleus and the cytosol. The protein is capable of forming a heterodimeric complex that associates with ribosomes, acting as a molecular chaperone for nascent polypeptide chains as they exit the ribosome. This protein was identified as a leukemia-associated antigen and expression of the gene is upregulated in leukemic blasts. Also, chromosomal aberrations involving this gene are associated with primary head and neck squamous cell tumors. This gene has a pseudogene on chromosome 6. Alternatively spliced variants which encode different protein isoforms have been described. [provided by RefSeq, Jul 2008]

Function: Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb-repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation. Specifically binds DNA sequence 5'-GTCAAGC-3'. Ref.8 Ref.9 Ref.15

Subunit structure: Interacts (via ZRF1-UBD region) with ID1

By similarity. Component of ribosome-associated complex (RAC), a heterodimer composed of Hsp70/DnaK-type chaperone HSPA14 and Hsp40/DnaJ-type chaperone DNAJC2. Ref.8

Subcellular location: Nucleus. Cytoplasm › cytosol Ref.8 Ref.15.

Tissue specificity: Widely expressed. Ref.5

Induction: Expression is repressed by CEBPA. Strongly overexpressed in leukemic cells. Ref.6 Ref.7 Ref.11

Domain: The ZRF1-UBD region specifically recognizes and binds H2AK119ub. The ZRF1-UBD region is also involved in protein-protein interactions with other proteins, suggesting that it may be masked by some regulator, thereby preventing its association with H2AK119ub. Ref.15

Post-translational modification: Phosphorylated in M (mitotic) phase. Ref.1

Miscellaneous: Constitutes a myeloid leukemia-associated antigen and might be a target for leukemia T-cell therapy.

Sequence similarities: Contains 1 J domain.Contains 2 SANT domains.

Sequence caution: The sequence AAI39752.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence CAA66913.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.