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GPX5 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS648524

Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. May constitute a glutathione peroxidase-like protective system against peroxide damage in sperm… More details

$880 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GPX5, Monoclonal Antibody
Full Product Name
GPX5 (Epididymal Secretory Glutathione Peroxidase, Epididymis-specific Glutathione Peroxidase-like Protein, EGLP, Glutathione Peroxidase 5, GPx-5, GSHPx-5)
Product Gene Name
Product Synonym Names
Anti -GPX5 (Epididymal Secretory Glutathione Peroxidase, Epididymis-specific Glutathione Peroxidase-like Protein, EGLP, Glutathione Peroxidase 5, GPx-5, GSHPx-5)
Immunogen Sequence
VRPGGGFVPS FQLFEKGDVN GEKEQKVFSF LKHSCPHPSE ILGTFKSISW DPVKVHDIRW NFEKFLVGPD GIPVMRWSHR ATVSSVKTDI LAYLKQFKTK
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
3B9
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human GPX5.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
25,202 Da
Chromosome Location
Chromosome: 6; NC_000006.11 (28493789..28502728). Location: 6p22.1
OMIM
603435
3D Structure
ModBase 3D Structure for O75715
Immunogen
Partial recombinant corresponding to aa122-221 from human GPX5 (NP_001500) with GST tag. MW of the GST tag alone is 26kD.
Applications Tested/Suitable for anti-GPX5 antibody
ELISA (EL/EIA), Immunoprecipitation (IP)
Application Notes for anti-GPX5 antibody
Suitable for use in ELISA and Immunoprecipitation.

Immunoprecipitation (IP) of anti-GPX5 antibody

Immunoprecipitation of GPX5 transfected lysate using GPX5 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with GPX5 rabbit polyclonal antibody.
Mouse Anti-GPX5 Monoclonal Antibody – Cat# MBS648524

Testing Data of anti-GPX5 antibody

Detection limit for recombinant GST tagged GPX5 is 1ng/ml as a capture antibody.
Mouse Anti-GPX5 Monoclonal Antibody, Testing Data – Cat# MBS648524

Other Notes

Small volumes of anti-GPX5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GPX5 purchase

MBS648524 is an antibody targeting GPX5. Also known as GPX5 (Epididymal Secretory Glutathione Peroxidase, Epididymis-specific Glutathione Peroxidase-like Protein, EGLP, Glutathione Peroxidase 5, GPx-5, GSHPx-5). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for GPX5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
glutathione peroxidase type 5 (GPX5)
NCBI Official Synonym Full Names
glutathione peroxidase 5 (epididymal androgen-related protein)
NCBI Official Symbol
GPX5
UniProt Protein Name
Epididymal secretory glutathione peroxidase
UniProt Synonym Protein Names
Epididymis-specific glutathione peroxidase-like protein; EGLP; Glutathione peroxidase 5
UniProt Gene Name
GPX5
UniProt Synonym Gene Names
EGLP; GPx-5; GSHPx-5
UniProt Entry Name
GPX5_HUMAN
NCBI Protein Information
epididymal secretory glutathione peroxidase; EGLP; GPx-5; GSHPx-5; epididymal androgen-related protein; epididymis-specific glutathione peroxidase-like protein

NCBI Summary for GPX5

This gene belongs to the glutathione peroxidase family. It is specifically expressed in the epididymis in the mammalian male reproductive tract, and is androgen-regulated. Unlike mRNAs for other characterized glutathione peroxidases, this mRNA does not contain a selenocysteine (UGA) codon. Thus, the encoded protein is selenium-independent, and has been proposed to play a role in protecting the membranes of spermatozoa from the damaging effects of lipid peroxidation and/or preventing premature acrosome reaction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2008]
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UniProt Comments for GPX5

Function: Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. May constitute a glutathione peroxidase-like protective system against peroxide damage in sperm membrane lipids.

Catalytic activity: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O.

Subcellular location: Secreted.

Tissue specificity: Epididymis.

Sequence similarities: Belongs to the glutathione peroxidase family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GPX5 antibody

Organs/Tissues associated with anti-GPX5 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >9 publications with GPX5 and Testis
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