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Lipoprotein Receptor-Related Protein Associated Protein 1, Low Density (LRPAP1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6485300

MBS6485300 is an antibody targeting LRPAP1. Also known as Lipoprotein Receptor-related Protein Associated Protein 1, Low Density, CT (Low Density Lipoprotein Receptor-related Protein Associated Protein 1, LRPAP1, Alpha-2-macroglobulin Receptor-associated… More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Lipoprotein Receptor-related Protein Associated Protein 1, Low Density (LRPAP1), Polyclonal Antibody
Full Product Name
Lipoprotein Receptor-related Protein Associated Protein 1, Low Density, CT (Low Density Lipoprotein Receptor-related Protein Associated Protein 1, LRPAP1, Alpha-2-macroglobulin Receptor-associated Protein, Alpha-2-MRAP, A2MRAP, A2RAP, HBP44, MGC138272, MR
Product Gene Name
Product Synonym Names
Lipoprotein Receptor Associated Protein
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography
Specificity
Recognizes human LRPAP1.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
160,000
Chromosome Location
Chromosome: 4; NC_000004.11 (3505324..3534224, complement). Location: 4p16.3
OMIM
gene 615431
3D Structure
ModBase 3D Structure for P30533
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 281-309 in the C-terminal region of human LRPAP1, UniProt Accession #P30533
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-LRPAP1 antibody

Applications Tested/Suitable for anti-LRPAP1 antibody
FLISA, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-LRPAP1 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-LRPAP1 antibody

Western Blot analysis of MBS629244 in Hela,HepG2 cell line lysates (35ug/lane).This demonstrates that MBS629244 detected the LRPAP1 protein (arrow).
Rabbit Anti-LRPAP1 Polyclonal Antibody – Cat# MBS6485300

Immunohistochemistry (IHC) of anti-LRPAP1 antibody

Formalin-fixed and paraffin-embedded human brain tissue withMBS629244, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS629244 for immunohistochemistry.
Rabbit Anti-LRPAP1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6485300

Flow Cytometry (FC/FACS) of anti-LRPAP1 antibody

Flow cytometric analysis of HepG2 cells using MBS629244 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-LRPAP1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6485300

Product References and Citations for anti-LRPAP1 antibody

1. Lisi, S., et.al., J. Endocrinol. Invest. 30(10), 839-843 (2007). 2. Vertegaal, A.C., et.al., Mol. Cell Proteomics 5(12), 2298-2310 (2006).

Other Notes

Small volumes of anti-LRPAP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for LRPAP1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
alpha-2-macroglobulin receptor-associated protein
NCBI Official Synonym Full Names
low density lipoprotein receptor-related protein associated protein 1
NCBI Official Symbol
LRPAP1
NCBI Official Synonym Symbols
RAP; MRAP; A2RAP; HBP44; A2MRAP
UniProt Protein Name
Alpha-2-macroglobulin receptor-associated protein
UniProt Synonym Protein Names
Low density lipoprotein receptor-related protein-associated protein 1; RAP
UniProt Gene Name
LRPAP1
UniProt Synonym Gene Names
A2MRAP; Alpha-2-MRAP; RAP
UniProt Entry Name
AMRP_HUMAN
NCBI Protein Information
alpha-2-macroglobulin receptor-associated protein; alpha-2-MRAP; lipoprotein receptor associated protein; alpha-2-macroglobulin receptor-associated protein 1; low density lipoprotein receptor-related protein-associated protein 1; low density lipoprotein-r

NCBI Summary for LRPAP1

This gene encodes a protein that interacts with the low density lipoprotein (LDL) receptor-related protein and facilitates its proper folding and localization by preventing the binding of ligands. Mutations in this gene have been identified in individuals with myopia 23. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
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UniProt Comments for LRPAP1

LRPAP1: Interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330. In complex with the alpha-2-MR or gp330, it may have some role in the pathogenesis of membrane glomerular nephritis. Belongs to the alpha-2-MRAP family.

Protein type: Receptor, misc.

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: cell surface; endoplasmic reticulum; extracellular region; plasma membrane; integral to membrane; vesicle

Molecular Function: heparin binding; receptor antagonist activity; protein binding; low-density lipoprotein receptor binding; unfolded protein binding; asialoglycoprotein receptor activity

Biological Process: vesicle-mediated transport; receptor-mediated endocytosis; protein folding; negative regulation of protein binding

Disease: Myopia 23, Autosomal Recessive
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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