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EXO5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS648535

MBS648535 is an antibody targeting EXO5. Also known as DEM1, ID (DEM1, C1orf176, Probable exonuclease V, Defects in morphology protein 1 homolog). Reactive in Hamster, Human, Mouse. Host: Rabbit. More details

$895 / 0.2 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
DEM1 (EXO5), Polyclonal Antibody
Full Product Name
DEM1, ID (DEM1, C1orf176, Probable exonuclease V, Defects in morphology protein 1 homolog)
Product Gene Name
anti-EXO5 antibody
Product Synonym Names
Anti -DEM1, ID (DEM1, C1orf176, Probable exonuclease V, Defects in morphology protein 1 homolog)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Hamster, Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human, hamster, mouse
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
41,816 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (40974391..40982228). Location: 1p34.2
3D Structure
ModBase 3D Structure for Q9H790
Immunogen
DEM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 173-201 amino acids from the Central region of human DEM1.

Product Categories/Family for anti-EXO5 antibody

Antibodies; Abs to KIAA; orf
Applications Tested/Suitable for anti-EXO5 antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-EXO5 antibody
Suitable for use in Western Blot, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-EXO5 antibody

DEM1 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the DEM1 antibody detected the DEM1 protein (arrow).
Rabbit Anti-EXO5 Polyclonal Antibody – Cat# MBS648535

Western Blot (WB) of anti-EXO5 antibody

DEM1 Antibody (Center) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the DEM1 antibody detected the DEM1 protein (arrow).
Rabbit Anti-EXO5 Polyclonal Antibody, Western Blot (WB) – Cat# MBS648535

Western Blot (WB) of anti-EXO5 antibody

DEM1 Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the DEM1 antibody detected the DEM1 protein (arrow).
Rabbit Anti-EXO5 Polyclonal Antibody, Western Blot (WB) – Cat# MBS648535

Flow Cytometry (FC/FACS) of anti-EXO5 antibody

DEM1 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-EXO5 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS648535

Other Notes

Small volumes of anti-EXO5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EXO5. It may not necessarily be applicable to this product.

NCBI GI #
18314555
NCBI GeneID
64789
NCBI Accession #
AAH21969.1
UniProt Primary Accession #
Q9H790
UniProt Secondary Accession #
Q5SWM7; Q5SWM8; Q5SWM9; Q5SWN0; Q5SWN1; Q8WTW9; D3DPV4
UniProt Related Accession #
Q9H790
NCBI Official Full Name
DEM1 protein
NCBI Official Synonym Full Names
exonuclease 5
NCBI Official Symbol
EXO5
NCBI Official Synonym Symbols
DEM1; Exo V; hExo5; C1orf176
UniProt Protein Name
Exonuclease V
UniProt Synonym Protein Names
Defects in morphology protein 1 homolog
Protein Family
Exonuclease
UniProt Gene Name
EXO5
UniProt Synonym Gene Names
C1orf176; DEM1; Exo V; hExo5
UniProt Entry Name
EXO5_HUMAN
NCBI Protein Information
exonuclease V; probable exonuclease V; defects in morphology 1 homolog; defects in morphology protein 1 homolog

UniProt Comments for EXO5

Function: Single-stranded DNA (ssDNA) bidirectional exonuclease involved in DNA repair. Probably involved in DNA repair following ultraviolet (UV) irradiation and interstrand cross-links (ICLs) damage. Has both 5'-3' and 3'-5' exonuclease activities with a strong preference for 5'-ends. Acts as a sliding exonuclease that loads at ssDNA ends and then slides along the ssDNA prior to cutting; however the sliding and the 3'-5' exonuclease activities are abolished upon binding to the replication protein A (RPA) complex that enforces 5'-directionality activity. Ref.5

Cofactor: Binds 1 4Fe-4S cluster. Ref.5Magnesium

By similarity. Ref.5

Subunit structure: Monomer; monomeric form has weak exonuclease activity. Homodimer; homodimeric form is unsure but has much higher exonuclease activity, suggesting that it could homodimerize upon DNA-binding. Interacts with the replication protein A (RPA) complex. Ref.5

Subcellular location: Nucleus. Cytoplasm › cytosol. Note: Localizes to repair foci in response to DNA damage. Ref.5

Sequence similarities: Belongs to the EXO5 family.

Sequence caution: The sequence CAI16298.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI16299.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI16300.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI16301.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI16302.1 differs from that shown. Reason: Erroneous gene model prediction.
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Research Articles on EXO5

1. The human homolog (C1orf176; EXO5) that functions in the repair of nuclear DNA damage.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-EXO5 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1 publications with EXO5 and Neoplasms
 Abnormalities, Drug-Induced Antibodies  >1 publications with EXO5 and Abnormalities, Drug-Induced
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