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LSD1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6485471

MBS6485471 is an antibody targeting LSD1. Also known as LSD1, NT (Lysine-specific Histone Demethylase 1A, Amine Oxidase Flavin Containing Domain Protein 2, BRAF35-HDAC Complex Protein BHC110, AOF2, KIAA0601, KDM1) (MaxLight 750). Reactive in Human, Mouse.… More details

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Product Overview

Online Datasheet in PDF
Product Name
LSD1, Polyclonal Antibody
Full Product Name
LSD1, NT (Lysine-specific Histone Demethylase 1A, Amine Oxidase Flavin Containing Domain Protein 2, BRAF35-HDAC Complex Protein BHC110, AOF2, KIAA0601, KDM1) (MaxLight 750)
Product Gene Name
anti-LSD1 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Specificity
Recognizes human LSD1. Species Crossreactivity: mouse.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
92.903
OMIM
609132
3D Structure
ModBase 3D Structure for O60341
Immunogen
Synthetic peptide selected from the N-term of human LSD1 (KLH).
Conjugate
MaxLight750
Note
Preservative Free

Product Categories/Family for anti-LSD1 antibody

Antibodies; Enzymes; Deacetylase
Applications Tested/Suitable for anti-LSD1 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes for anti-LSD1 antibody
IHC: 1:50-1:100
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-LSD1 antibody

Western blot analysis of anti-LSD1 Pab in mouse brain tissue lysate (35ug/lane). LSD1(arrow) was detected using the purified Pab.
Rabbit Anti-LSD1 Polyclonal Antibody – Cat# MBS6485471

Western Blot (WB) of anti-LSD1 antibody

Western blot analysis of AOF2 (arrow) using LSD1 Antibody (N-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AOF2 gene (Lane 2) (Origene Technologies).
Rabbit Anti-LSD1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6485471

Immunohistochemistry (IHC) of anti-LSD1 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-LSD1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6485471

Immunofluorescence (IF) of anti-LSD1 antibody

Fluorescent confocal image of Hela cell stained with LSD1 Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with LSD1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). LSD1 immunoreactivity is localized to Cytoplasm significantly and Nucleus weakly.
Rabbit Anti-LSD1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6485471

Product References and Citations for anti-LSD1 antibody

1. Forneris, F., et al. FEBS Lett. 579 (10), 2203-2207 (2005). 2. Shi, Y., et al. Cell 119 (7), 941-953 (2004). 3. Hakimi, M.A., et al. J. Biol. Chem. 278 (9), 7234-7239 (2003). 4. Hakimi, M.A., et al. PNAS 99 (11), 7420-7425 (2002). 5. Humphrey, G.W., et al. J. Biol. Chem. 276 (9), 6817-6824 (2001). 6. Ota, T., et al., Nat. Genet. 36(1):40-45 (2004).

Other Notes

Small volumes of anti-LSD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for LSD1. It may not necessarily be applicable to this product.

NCBI GI #
58761546
NCBI GeneID
23028
NCBI Accession #
NP_055828
UniProt Primary Accession #
O60341
UniProt Related Accession #
O60341
GenBank Nucleotide #
NP_055828.2
NCBI Official Full Name
lysine-specific histone demethylase 1A isoform b
NCBI Official Synonym Full Names
lysine demethylase 1A
NCBI Official Symbol
KDM1A
NCBI Official Synonym Symbols
AOF2; CPRF; KDM1; LSD1; BHC110
UniProt Protein Name
Lysine-specific histone demethylase 1A
UniProt Synonym Protein Names
BRAF35-HDAC complex protein BHC110; Flavin-containing amine oxidase domain-containing protein 2
Protein Family
Lysine-specific histone demethylase
UniProt Gene Name
KDM1A
UniProt Synonym Gene Names
AOF2; KDM1; KIAA0601; LSD1
UniProt Entry Name
KDM1A_HUMAN
NCBI Protein Information
lysine-specific histone demethylase 1A

NCBI Summary for LSD1

This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
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Research Articles on LSD1

1. Over-expressed LSD1 is related with poor overall survival of the hepatocellular carcinoma patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-LSD1 antibody

 Products by Pathway  Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Chromatin Modifying Enzymes Pathway antibodies  Chromatin Modifying Enzymes Pathway Diagram
 Chromatin Organization Pathway antibodies  Chromatin Organization Pathway Diagram
 Coregulation Of Androgen Receptor Activity Pathway antibodies  Coregulation Of Androgen Receptor Activity Pathway Diagram
 Factors Involved In Megakaryocyte Development And Platelet Production Pathway antibodies  Factors Involved In Megakaryocyte Development And Platelet Production Pathway Diagram
 HDACs Deacetylate Histones Pathway antibodies  HDACs Deacetylate Histones Pathway Diagram
 HDMs Demethylate Histones Pathway antibodies  HDMs Demethylate Histones Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Notch Signaling Pathway antibodies  Notch Signaling Pathway Diagram
 Notch-mediated HES/HEY Network Pathway antibodies  Notch-mediated HES/HEY Network Pathway Diagram

Diseases associated with anti-LSD1 antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >17 publications with LSD1 and Breast Neoplasms
 Disease Models, Animal Antibodies  >8 publications with LSD1 and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >8 publications with LSD1 and Cardiovascular Diseases
 Neoplasm Invasiveness Antibodies  >8 publications with LSD1 and Neoplasm Invasiveness
 Lymphoma Antibodies  >7 publications with LSD1 and Lymphoma
 Nervous System Diseases Antibodies  >5 publications with LSD1 and Nervous System Diseases
 Liver Diseases Antibodies  >5 publications with LSD1 and Liver Diseases
 Liver Neoplasms Antibodies  >4 publications with LSD1 and Liver Neoplasms
 Disease Progression Antibodies  >4 publications with LSD1 and Disease Progression
 Hypertension Antibodies  >4 publications with LSD1 and Hypertension

Organs/Tissues associated with anti-LSD1 antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >26 publications with LSD1 and Nerve
 Brain Antibodies  >13 publications with LSD1 and Brain
 Prostate Antibodies  >11 publications with LSD1 and Prostate
 Blood Antibodies  >11 publications with LSD1 and Blood
 Embryonic Tissue Antibodies  >7 publications with LSD1 and Embryonic Tissue
 Bone Antibodies  >7 publications with LSD1 and Bone
 Lung Antibodies  >6 publications with LSD1 and Lung
 Liver Antibodies  >6 publications with LSD1 and Liver
 Heart Antibodies  >5 publications with LSD1 and Heart
 Muscle Antibodies  >5 publications with LSD1 and Muscle
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