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OPN Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS648553

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of… More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
OPN-a/b, Polyclonal Antibody
Full Product Name
OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin)
Product Synonym Names
Anti -OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
OPN-a/b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-48 amino acids from the N-terminal region of human OPN-a/b.

Product Categories/Family for anti-OPN-a/b antibody

Antibodies; Abs to Proteins
Applications Tested/Suitable for anti-OPN-a/b antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-OPN-a/b antibody
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Immunofluorescence: 1:10-50

Western Blot (WB) of anti-OPN-a/b antibody

Western Blot analysis of OPN-a/b (arrow) using MBS648553. 293 cell lysates (2ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the OPN-a/b gene.
Rabbit Anti-OPN Polyclonal Antibody – Cat# MBS648553

Testing Data of anti-OPN-a/b antibody

All lanes: MBS648553 at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: MOLT-4 whole cell lysate Lysates/proteins at 20ug/lane. Secondary IgG (H+L) goat anti-rabbit, Peroxidase conjugated at 1:10000 dilution. Predicted band size: 35kD Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-OPN Polyclonal Antibody, Testing Data – Cat# MBS648553

Testing Data of anti-OPN-a/b antibody

MBS648553 at 1:2000 dilution + Hela whole cell lysate Lysates/proteins at 20ug/lane. Secondary IgG, (H+L) goat anti-rabbit Peroxidase conjugated at 1:10000 dilution. Predicted band size: 35kD Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-OPN Polyclonal Antibody, Testing Data – Cat# MBS648553

Testing Data of anti-OPN-a/b antibody

All lanes: MBS648553 at 1:1000 dilution Lane 1: human liver lysates Lane 2: MDA-MB-453 whole cell lysates Lane 3: MOLT-4 whole cell lysates Lysates/proteins at 20ug/lane. Secondary IgG, (H+L) goat anti-rabbit, Peroxidase conjugated at 1:10000 dilution. Predicted band size : 35kD Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-OPN Polyclonal Antibody, Testing Data – Cat# MBS648553

Immunohistochemistry (IHC) of anti-OPN-a/b antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue using MBS648553 followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MBS648553 for immunohistochemistry.
Rabbit Anti-OPN Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS648553

Immunofluorescence (IF) of anti-OPN-a/b antibody

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with MBS648553 at 1:25 dilution, followed by Dylight® 488-conjugated IgG goat anti-rabbit secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).
Rabbit Anti-OPN Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS648553

Other Notes

Small volumes of anti-OPN-a/b antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for OPN-a/b purchase

MBS648553 is an antibody targeting OPN-a/b. Also known as OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin). Reactive in Human. Host: Rabbit.
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