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PLB Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS648568

PLB is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains… More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PLB-T17, Polyclonal Antibody
Full Product Name
PLB-T17 (PLN, PLB, Cardiac phospholamban)
Product Synonym Names
Anti -PLB-T17 (PLN, PLB, Cardiac phospholamban)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
PLB-T17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the S16 region of human PLB.
Applications Tested/Suitable for anti-PLB-T17 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-PLB-T17 antibody
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-PLB-T17 antibody

Western Blot analysis in Jurkat cell line lysates (35ug/lane) using MBS648568. PLB (arrow) was detected using the purified Pab.
Rabbit Anti-PLB Polyclonal Antibody – Cat# MBS648568

Immunohistochemistry (IHC) of anti-PLB-T17 antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue using MBS648568, followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS648568 for immunohistochemistry.
Rabbit Anti-PLB Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS648568

Flow Cytometry (FC/FACS) of anti-PLB-T17 antibody

Flow Cytometric analysis of Jurkat cells (bottom histogram) using MBS648568 compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-PLB Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS648568

Other Notes

Small volumes of anti-PLB-T17 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PLB-T17 purchase

MBS648568 is an antibody targeting PLB-T17. Also known as PLB-T17 (PLN, PLB, Cardiac phospholamban). Reactive in Human. Host: Rabbit.
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