OS9 Antibody – Rabbit Polyclonal

Function: Lectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4. Ref.10 Ref.11 Ref.13 Ref.14 Ref.15 Ref.17

Subunit structure: Probably part of the HRD1 ubiquitin ligase complex composed at least of SYVN1/HRD1 and SEL1L with which it interacts directly. Through this complex it may interact with ERLEC1 and HSPA5. Interacts with DERL2. Interacts with HSP90B1 and CREB3. Ref.12 Ref.13 Ref.15 Ref.16

Subcellular location: Endoplasmic reticulum lumen Ref.11 Ref.13 Ref.15.

Tissue specificity: Ubiquitously expressed. Found as well in all tumor cell lines analyzed, amplified in sarcomas. Highly expressed in osteosarcoma SJSA-1 and rhabdomyosarcoma Rh30 cell lines. Isoform 2 is the major isoform detected in all cell types examined.

Induction: Up-regulated in response to endoplasmic reticulum stress (at protein level). Ref.11 Ref.15

Post-translational modification: Intramolecular disulfide bonds.Isoform 1 and isoform 2 are N-glycosylated. Ref.11 Ref.13 Ref.15

Sequence similarities: Belongs to the OS-9 family.Contains 1 PRKCSH domain.