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DGKK Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS648738

MBS648738 is an antibody targeting DGKK. Also known as DGKK, CT (DGKK, Diacylglycerol kinase kappa, 142kD diacylglycerol kinase, Diglyceride kinase kappa). Reactive in Human, Mouse. Host: Rabbit. DGKK phosphorylates diacylglycerol (DAG) to generate… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
DGKK, Polyclonal Antibody
Full Product Name
DGKK, CT (DGKK, Diacylglycerol kinase kappa, 142kD diacylglycerol kinase, Diglyceride kinase kappa)
Product Gene Name
anti-DGKK antibody
Product Synonym Names
Anti -DGKK, CT (DGKK, Diacylglycerol kinase kappa, 142kD diacylglycerol kinase, Diglyceride kinase kappa)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human, mouse
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
141,829 Da
Chromosome Location
Chromosome: X; NC_000023.10 (50111879..50213721, complement). Location: Xp11.22
OMIM
300837
3D Structure
ModBase 3D Structure for Q5KSL6
Immunogen
DGKK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1241-1271 amino acids from the C-terminal region of human DGKK.

Product Categories/Family for anti-DGKK antibody

Antibodies; Abs to Protein Kinases
Applications Tested/Suitable for anti-DGKK antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-DGKK antibody
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100

Western Blot (WB) of anti-DGKK antibody

Western blot analysis of DGKK Antibody (C-term) in mouse lung tissue lysates (35ug/lane).DGKK (arrow) was detected using the purified Pab.
Rabbit Anti-DGKK Polyclonal Antibody – Cat# MBS648738

Immunohistochemistry (IHC) of anti-DGKK antibody

DGKK Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DGKK Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-DGKK Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS648738

Other Notes

Small volumes of anti-DGKK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DGKK. It may not necessarily be applicable to this product.

NCBI GI #
62000702
NCBI GeneID
139189
NCBI Accession #
NP_001013764.1
UniProt Primary Accession #
Q5KSL6
UniProt Secondary Accession #
B2RP91
UniProt Related Accession #
Q5KSL6
GenBank Nucleotide #
NM_001013742.2
NCBI Official Full Name
diacylglycerol kinase kappa
NCBI Official Synonym Full Names
diacylglycerol kinase, kappa
NCBI Official Symbol
DGKK
UniProt Protein Name
Diacylglycerol kinase kappa
UniProt Synonym Protein Names
142 kDa diacylglycerol kinase; Diglyceride kinase kappa
Protein Family
Diacylglycerol kinase
UniProt Gene Name
DGKK
UniProt Synonym Gene Names
DAG kinase kappa; DGK-kappa
UniProt Entry Name
DGKK_HUMAN
NCBI Protein Information
diacylglycerol kinase kappa; DGK-kappa; DAG kinase kappa; diglyceride kinase kappa; 142 kDa diacylglycerol kinase

NCBI Summary for DGKK

The protein encoded by this gene is an enzyme that phosphorylates diacylglycerol, converting it to phosphatidic acid. The encoded protein is a membrane protein and is inhibited by hydrogen peroxide. Variations in this gene have been associated with hypospadias. [provided by RefSeq, Mar 2011]

UniProt Comments for DGKK

Function: Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Ref.1

Catalytic activity: ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate. Ref.1

Enzyme regulation: Inhibited in response to H2O2. Ref.1

Subunit structure: Does not form homooligomers.

Subcellular location: Cytoplasm. Membrane; Peripheral membrane protein Ref.1.

Tissue specificity: Expressed in testis, and to a lesser extent in placenta. Ref.1

Post-translational modification: Phosphorylated at Tyr-78 by some member of the SRC family in response to H2O2. Ref.1

Sequence similarities: Belongs to the eukaryotic diacylglycerol kinase family.Contains 1 DAGKc domain.Contains 1 PH domain.Contains 2 phorbol-ester/DAG-type zinc fingers.
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Research Articles on DGKK

1. Common variants in DGKK are strongly associated with risk of hypospadias

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-DGKK antibody

 Products by Pathway  Pathway Diagram
 Effects Of PIP2 Hydrolysis Pathway antibodies  Effects Of PIP2 Hydrolysis Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram

Diseases associated with anti-DGKK antibody

 Disease Name  Pubmed Publications
 Hypospadias Antibodies  >3 publications with DGKK and Hypospadias
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