• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

NR0B2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS648765

The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. The gene product is a member of the nuclear hormone receptor family, a group of transcription factors… More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NR0B2, Polyclonal Antibody
Full Product Name
NR0B2, ID (NR0B2, SHP, Nuclear receptor subfamily 0 group B member 2, Orphan nuclear receptor SHP, Small heterodimer partner)
Product Gene Name
anti-NR0B2 antibody
Product Synonym Names
Anti -NR0B2, ID (NR0B2, SHP, Nuclear receptor subfamily 0 group B member 2, Orphan nuclear receptor SHP, Small heterodimer partner)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
28,058 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (27237975..27240567, complement). Location: 1p36.1
OMIM
601665
3D Structure
ModBase 3D Structure for Q15466
Immunogen
NR0B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-83 amino acids from the Central region of human NR0B2.

Product Categories/Family for anti-NR0B2 antibody

Antibodies; Abs to Receptors
Applications Tested/Suitable for anti-NR0B2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-NR0B2 antibody
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Immunofluorescence: 1:10-50
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-NR0B2 antibody

NR0B2 Antibody (Center)(PEI 1:100) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the NR0B2 antibody detected the NR0B2 protein (arrow).
Rabbit Anti-NR0B2 Polyclonal Antibody – Cat# MBS648765

Immunohistochemistry (IHC) of anti-NR0B2 antibody

NR0B2 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NR0B2 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-NR0B2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS648765

Immunofluorescence (IF) of anti-NR0B2 antibody

Confocal immunofluorescent analysis of NR0B2 Antibody (Center) with HepG2 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-NR0B2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS648765

Flow Cytometry (FC/FACS) of anti-NR0B2 antibody

NR0B2 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-NR0B2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS648765

Other Notes

Small volumes of anti-NR0B2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NR0B2 purchase

MBS648765 is an antibody targeting NR0B2. Also known as NR0B2, ID (NR0B2, SHP, Nuclear receptor subfamily 0 group B member 2, Orphan nuclear receptor SHP, Small heterodimer partner). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NR0B2. It may not necessarily be applicable to this product.

NCBI GI #
13259503
NCBI GeneID
8431
NCBI Accession #
NP_068804.1
UniProt Primary Accession #
Q15466
UniProt Secondary Accession #
Q5QP36
UniProt Related Accession #
Q15466
GenBank Nucleotide #
NM_021969.2
NCBI Official Full Name
nuclear receptor subfamily 0 group B member 2
NCBI Official Synonym Full Names
nuclear receptor subfamily 0, group B, member 2
NCBI Official Symbol
NR0B2
NCBI Official Synonym Symbols
SHP; SHP1
UniProt Protein Name
Nuclear receptor subfamily 0 group B member 2
UniProt Synonym Protein Names
Orphan nuclear receptor SHP; Small heterodimer partner
Protein Family
Nuclear receptor subfamily
UniProt Gene Name
NR0B2
UniProt Synonym Gene Names
SHP
UniProt Entry Name
NR0B2_HUMAN
NCBI Protein Information
nuclear receptor subfamily 0 group B member 2; nuclear receptor SHP; small heterodimer partner; orphan nuclear receptor SHP

UniProt Comments for NR0B2

Function: Acts as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated trancription complex for NEUROD1. Ref.6

Subunit structure: Interacts with RARA, RXRA, THRB, NR5A1, NR5A2, NR1I3, PPARA, PPARG and EID1. May also interact with HNF4A

By similarity. Heterodimer; efficient DNA binding requires dimerization with another bHLH protein. Interacts (via N-terminus) with NEUROD1 (via N-terminus an C-terminus). Interacts with ID2. Ref.6

Subcellular location: Nucleus. Cytoplasm. Note: Colocalizes with NEUROD1 in the nucleus. Ref.6

Tissue specificity: Liver. Low levels of expression were detected in heart and pancreas. Ref.1

Involvement in disease: Obesity (OBESITY) [MIM:601665]: A condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Ref.3

Sequence similarities: Belongs to the nuclear hormone receptor family. NR0 subfamily.
Show more

Research Articles on NR0B2

1. HNF4alpha and LRH-1 promote active transcription histone marks on the Cyp7a1 promoter that are reversed by FGF19 in a SHP-dependent manner

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NR0B2 antibody

 Reference Product  PubMed Publications
 NR5A2 antibody  >6 publications with NR0B2 and NR5A2
 PPARG antibody  >2 publications with NR0B2 and PPARG
 HNF4A antibody  >2 publications with NR0B2 and HNF4A
 ESRRG antibody  >2 publications with NR0B2 and ESRRG
 ESR1 antibody  >1 publications with NR0B2 and ESR1

Pathways associated with anti-NR0B2 antibody

 Products by Pathway  Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Nuclear Receptor Transcription Pathway antibodies  Nuclear Receptor Transcription Pathway Diagram

Diseases associated with anti-NR0B2 antibody

 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >13 publications with NR0B2 and Liver Diseases
 Obesity Antibodies  >12 publications with NR0B2 and Obesity
 Neoplasms Antibodies  >11 publications with NR0B2 and Neoplasms
 Liver Neoplasms Antibodies  >10 publications with NR0B2 and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >6 publications with NR0B2 and Carcinoma, Hepatocellular
 Fatty Liver Antibodies  >5 publications with NR0B2 and Fatty Liver
 Inflammation Antibodies  >2 publications with NR0B2 and Inflammation
 Fibrosis Antibodies  >2 publications with NR0B2 and Fibrosis
 Weight Loss Antibodies  >1 publications with NR0B2 and Weight Loss
 Hepatitis Antibodies  >1 publications with NR0B2 and Hepatitis

Organs/Tissues associated with anti-NR0B2 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >28 publications with NR0B2 and Liver
 Kidney Antibodies  >3 publications with NR0B2 and Kidney
 Heart Antibodies  >2 publications with NR0B2 and Heart
 Intestine Antibodies  >1 publications with NR0B2 and Intestine
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000