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ZBTB14 Antibody – Mouse Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS648776

MBS648776 is an antibody targeting ZBTB14. Also known as ZBTB14 (Zinc Finger and BTB Domain-containing Protein 14, Zinc Finger Protein 5 Homolog, Zfp-5, hZF5, ZF5, Zinc Finger Protein 161 Homolog, ZFP161, Zfp-161, Zinc Finger Protein 478, ZNF478). Reactive in… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ZBTB14, Polyclonal Antibody
Full Product Name
ZBTB14 (Zinc Finger and BTB Domain-containing Protein 14, Zinc Finger Protein 5 Homolog, Zfp-5, hZF5, ZF5, Zinc Finger Protein 161 Homolog, ZFP161, Zfp-161, Zinc Finger Protein 478, ZNF478)
Product Gene Name
Product Synonym Names
Anti -ZBTB14 (Zinc Finger and BTB Domain-containing Protein 14, Zinc Finger Protein 5 Homolog, Zfp-5, hZF5, ZF5, Zinc Finger Protein 161 Homolog, ZFP161, Zfp-161, Zinc Finger Protein 478, ZNF478)
Immunogen Sequence
MEFFISMSET IKYNDDDHKT LFLKTLNEQR LEGEFCDIAI VVEDVKFRAH RCVLAACSTY FKKLFKKLEV DSSSVIEIDF LRSDIFEEVL NYMYTAKISV KKEDVNLMMS SGQILGIRFL DKLCSQKRDV SSPDENNGQS KSKYCLKINR PIGDAADTQD DDVEEIGDQD DSPSDDTVEG TPPSQEDGKS PTTTLRVQEA ILKELGSEEV RKVNCYGQEV ESMETPESKD LGSQTPQALT FNDGMSEVKD EQTPGWTTAA SDMKFEYLLY GHHREQIACQ ACGKTFSDEG RLRKHEKLHT ADRPFVCEMC TKGFTTQAHL KEHLKIHTGY KPYSCEVCGK SFIRAPDLKK HERVHSNERP FACHMCDKAF KHKSHLKDHE RRHRGEKPFV CGSCTKAFAK ASDLKRHENN MHSERKQVTP SAIQSETEQL QAAAMAAEAE QQLETIACS
Clonality
Polyclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human ZFP161.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
50,956 Da
Chromosome Location
Chromosome: 18; NC_000018.9 (5289018..5297052, complement). Location: 18p11.31
OMIM
602126
3D Structure
ModBase 3D Structure for O43829
Immunogen
Full length human ZFP161, aa1-449 (NP_003400.2).
Applications Tested/Suitable for anti-ZBTB14 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-ZBTB14 antibody
Suitable for use in Western Blot and Immunofluorescence.
Dilution: Immunofluorescence: 10ug/ml

Western Blot (WB) of anti-ZBTB14 antibody

Western Blot analysis of ZFP161 expression in transfected 293T cell line by ZFP161 polyclonal antibody. Lane 1: ZFP161 transfected lysate (49.39kD). Lane 2: Non-transfected lysate.
Mouse Anti-ZBTB14 Polyclonal Antibody – Cat# MBS648776

Immunofluorescence (IF) of anti-ZBTB14 antibody

Immunofluorescence of purified antibody to ZFP161 on HeLa cell. [antibody concentration 10ug/ml]
Mouse Anti-ZBTB14 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS648776

Other Notes

Small volumes of anti-ZBTB14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ZBTB14. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
zinc finger and BTB domain-containing protein 14
NCBI Official Synonym Full Names
zinc finger and BTB domain containing 14
NCBI Official Symbol
ZBTB14
NCBI Official Synonym Symbols
ZF5; ZFP-5; ZFP161; ZNF478; ZFP-161
UniProt Protein Name
Zinc finger and BTB domain-containing protein 14
UniProt Synonym Protein Names
Zinc finger protein 161 homolog; Zfp-161; Zinc finger protein 478; Zinc finger protein 5 homolog
UniProt Gene Name
ZBTB14
UniProt Synonym Gene Names
ZFP161; ZNF478; Zfp-161; ZF5; Zfp-5; hZF5
UniProt Entry Name
ZBT14_HUMAN
NCBI Protein Information
zinc finger and BTB domain-containing protein 14; zinc finger protein 478; ZFP161 zinc finger protein; zinc finger protein 5 homolog; zinc finger protein 161 homolog; zinc finger protein homologous to Zfp161 in mouse

UniProt Comments for ZBTB14

Function: Transcriptional activator of the dopamine transporter (DAT), binding it's promoter at the consensus sequence 5'-CCTGCACAGTTCACGGA-3'. Binds to 5'-d(GCC)(n)-3' trinucleotide repeats in promoter regions and acts as a repressor of the FMR1 gene. Transcriptional repressor of MYC and thymidine kinase promoters. Ref.4

Subunit structure: Interacts with ZBTB21. Ref.7

Subcellular location: Nucleus. Note: Colocalizes with ZBTB21 in nucleus in HEK293 cells. Ref.7

Domain: The BTB/POZ domain seems to direct the protein to discrete regions in the nucleus.

Sequence similarities: Belongs to the krueppel C2H2-type zinc-finger protein family.Contains 1 BTB (POZ) domain.Contains 5 C2H2-type zinc fingers.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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