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SMPD2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS648823

SMPD2 converts sphingomyelin to ceramide and hydrolyzes 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (lyso-platelet-activating factor). The physiological substrate seems to be Lyso-PAF. More details

$880 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SMPD2, Monoclonal Antibody
Full Product Name
SMPD2 (Sphingomyelin Phosphodiesterase 2, Lyso-platelet-activating Factor-phospholipase C, Lyso-PAF-PLC, N-SMase, nSMase, Neutral Sphingomyelinase)
Product Gene Name
Product Synonym Names
Anti -SMPD2 (Sphingomyelin Phosphodiesterase 2, Lyso-platelet-activating Factor-phospholipase C, Lyso-PAF-PLC, N-SMase, nSMase, Neutral Sphingomyelinase)
Immunogen Sequence
MKPNFSLRLR IFNLNCWGIP YLSKHRADRM RRLGDFLNQE SFDLALLEEV WSEQDFQYLR QKLSPTYPAA HHFRSGIIGS GLCVFSKHPI QELTQHIYTL NGYPYMIHHG DWFSGKAVGL LVLHLSGMVL NAYVTHLHAE YNRQKDIYLA HRVAQAWELA QFIHHTSKKA DVVLLCGDLN MHPEDLGCCL LKEWTGLHDA YLETRDFKGS EEGNTMVPKN CYVSQQELKP FPFGVRIDYV LYKAVSGFYI SCKSFETTTG FDPHSGTPLS DHEALMATLF VRHSPPQQNP SSTHGPAERS PLMCVLKEAW TELGLGMAQA RWWATFASYV IGLGLLLLAL LCVLAAGGGA GEAAILLWTP SVGLVLWAGA FYLFHVQEVN GLYRAQAELQ HVLGRAREAQ DLGPEPQPAL LLGQQEGDRT KEQ
Clonality
Monoclonal
Isotype
IgG3,k
Clone Number
2C9
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human SMPD2.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
47,645 Da
Chromosome Location
Chromosome: 20; NC_005119.3 (47904390..47907503, complement). Location: 20q12
3D Structure
ModBase 3D Structure for Q9ET64
Immunogen
Full length recombinant corresponding to aa1-424 from human SMPD2 (AAH00038) with GST tag. MW of the GST tag alone is 26kD.
Applications Tested/Suitable for anti-Smpd2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-Smpd2 antibody
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml
Optimal dilutions to be detemined by the researcher.

Western Blot (WB) of anti-Smpd2 antibody

Western Blot detection against Immunogen (72.64kD).
Mouse Anti-SMPD2 Monoclonal Antibody – Cat# MBS648823

Immunohistochemistry (IHC) of anti-Smpd2 antibody

Immunoperoxidase of monoclonal antibody to SMPD2 on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3ug/ml].
Mouse Anti-SMPD2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS648823

Testing Data of anti-Smpd2 antibody

Detection limit for recombinant GST tagged SMPD2 is ~10ng/ml as a capture antibody.
Mouse Anti-SMPD2 Monoclonal Antibody, Testing Data – Cat# MBS648823

Other Notes

Small volumes of anti-Smpd2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Smpd2 purchase

MBS648823 is an antibody targeting Smpd2. Also known as SMPD2 (Sphingomyelin Phosphodiesterase 2, Lyso-platelet-activating Factor-phospholipase C, Lyso-PAF-PLC, N-SMase, nSMase, Neutral Sphingomyelinase). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Smpd2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
sphingomyelin phosphodiesterase 2
NCBI Official Synonym Full Names
sphingomyelin phosphodiesterase 2, neutral membrane
NCBI Official Symbol
Smpd2
NCBI Official Synonym Symbols
nSMase
UniProt Protein Name
Sphingomyelin phosphodiesterase 2
UniProt Synonym Protein Names
Lyso-platelet-activating factor-phospholipase C; Lyso-PAF-PLC; Neutral sphingomyelinase
UniProt Gene Name
Smpd2
UniProt Synonym Gene Names
Lyso-PAF-PLC; N-SMase; nSMase
UniProt Entry Name
NSMA_RAT
NCBI Protein Information
sphingomyelin phosphodiesterase 2; N-SMase; lyso-PAF-PLC; neutral sphingomyelinase; lyso-platelet-activating factor-phospholipase C

NCBI Summary for Smpd2

catalyzes the Mg2+ dependent hydrolysis of sphingomyelin to form N-acylsphingosine and choline phosphate; also has a lower activity for the hydrolysis of lyso-platelet activating factor to 1-alkylglycerol [RGD, Feb 2006]

UniProt Comments for Smpd2

Function: Converts sphingomyelin to ceramide. Hydrolyze 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (lyso-platelet-activating factor). The physiological substrate seems to be Lyso-PAF.

Catalytic activity: Sphingomyelin + H2O = N-acylsphingosine + phosphocholine.

Cofactor: Magnesium.

Pathway: Lipid metabolism; sphingolipid metabolism.

Subcellular location: Membrane; Multi-pass membrane protein.

Sequence similarities: Belongs to the neutral sphingomyelinase family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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