SPAG5 Antibody – Mouse Monoclonal

Function: Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase. Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. The astrin (SPAG5)-kinastrin (SKAP) complex promotes stable microtubule-kinetochore attachments. Ref.1 Ref.6 Ref.15

Subunit structure: Homodimer, with a globular head domain and a long stalk. Homooligomer; the globular head domains associate, resulting in aster-like structures. Binds to microtubules in the mitotic spindle. Interacts with DCLRE1B/Apollo. Part of an astrin (SPAG5)-kinastrin (SKAP) complex containing KNSTRN, SPAG5, PLK1, DYNLL1 and SGOL2. Interacts with KNSTRN. Ref.1 Ref.6 Ref.11 Ref.15

Subcellular location: Cytoplasm. Cytoplasm › cytoskeleton › spindle. Cytoplasm › cytoskeleton › spindle pole. Chromosome › centromere › kinetochore. Note: In a punctate pattern in interphase cells. During mitosis, detected at spindle poles during prophase, throughout the spindle in metaphase and anaphase, and at midzone microtubules in anaphase and telophase. Detected on kinetochores of chromosomes that have congressed. The astrin (SPAG5)-kinastrin (SKAP) complex localizes to the microtubule plus ends

By similarity. Ref.1 Ref.2 Ref.6 Ref.15

Tissue specificity: Highly expressed in testis. Detected at low levels in placenta, liver, pancreas, thymus and colon. Ref.2

Sequence caution: The sequence AAD02813.1 differs from that shown. Reason: Frameshift at position 1093. The sequence AAL06396.2 differs from that shown. Reason: Frameshift at position 1135.