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PIGX Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS648875

Essential component of glycosylphosphatidylinositol-mannosyltransferase 1 which transfers the first of the 4 mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase PIGM (By similarity). More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PIGX, Polyclonal Antibody
Full Product Name
PIGX, NT (PIGX, Phosphatidylinositol-glycan biosynthesis class X protein)
Product Gene Name
Product Synonym Names
Anti -PIGX, NT (PIGX, Phosphatidylinositol-glycan biosynthesis class X protein)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human, mouse
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
28,644 Da
Chromosome Location
Chromosome: 16; NC_000082.6 (32084416..32099727, complement). Location: 16 B2; 16
3D Structure
ModBase 3D Structure for Q99LV7
Immunogen
PIGX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-103 amino acids from the N-terminal region of human PIGX.
Applications Tested/Suitable for anti-Pigx antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-Pigx antibody
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100

Western Blot (WB) of anti-Pigx antibody

PIGX Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the PIGX antibody detected the PIGX protein (arrow).
Rabbit Anti-PIGX Polyclonal Antibody – Cat# MBS648875

Immunohistochemistry (IHC) of anti-Pigx antibody

PIGX antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PIGX antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-PIGX Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS648875

Other Notes

Small volumes of anti-Pigx antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Pigx purchase

MBS648875 is an antibody targeting Pigx. Also known as PIGX, NT (PIGX, Phosphatidylinositol-glycan biosynthesis class X protein). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Pigx. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
phosphatidylinositol-glycan biosynthesis class X protein isoform 2
NCBI Official Synonym Full Names
phosphatidylinositol glycan anchor biosynthesis, class X
NCBI Official Symbol
Pigx
NCBI Official Synonym Symbols
PIG-X; 2010319C14Rik
UniProt Protein Name
Phosphatidylinositol-glycan biosynthesis class X protein
UniProt Gene Name
Pigx
UniProt Synonym Gene Names
PIG-X
UniProt Entry Name
PIGX_MOUSE
NCBI Protein Information
phosphatidylinositol-glycan biosynthesis class X protein; phosphatidylinositol glycan, class X

NCBI Summary for Pigx

This gene encodes a type I transmembrane protein in the endoplasmic reticulum (ER). The protein is an essential component of glycosylphosphatidylinositol-mannosyltransferase I, which transfers the first of the four mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Studies in rat indicate that the protein is translated from a non-AUG translation initiation site. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for Pigx

Function: Essential component of glycosylphosphatidylinositol-mannosyltransferase 1 which transfers the first of the 4 mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase PIGM

By similarity.

Pathway: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.

Subunit structure: Interacts with PIGM

By similarity.

Subcellular location: Endoplasmic reticulum membrane; Single-pass type I membrane protein

By similarity.

Post-translational modification: N-glycosylated

By similarity.

Sequence similarities: Belongs to the PIGX family.

Sequence caution: The sequence BAC25223.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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