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ADM Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS648888

ADM, a hypotensive peptide found in human pheochromocytoma, consists of 52 amino acids, has 1 intramolecular disulfide bond, and shows a slight homology with the calcitonin gene-related peptide. It may function as a hormone in circulation control because it… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ADM, Polyclonal Antibody
Full Product Name
ADM, ID (ADM, AM, Adrenomedullin, Proadrenomedullin N-20 terminal peptide, ProAM N-terminal 20 peptide)
Product Gene Name
Product Synonym Names
Anti -ADM, ID (ADM, AM, Adrenomedullin, Proadrenomedullin N-20 terminal peptide, ProAM N-terminal 20 peptide)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
20,893 Da
Chromosome Location
Chromosome: 2; NC_010444.3 (52576219..52578518, complement). Location: chromosome: 2
3D Structure
ModBase 3D Structure for P53366
Immunogen
ADM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-105 amino acids from the Central region of human ADM.

Product Categories/Family for anti-ADM antibody

Applications Tested/Suitable for anti-ADM antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-ADM antibody
Suitable for use in Western Blot, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-ADM antibody

Western Blot analysis in mouse lung tissue lysates (35ug/lane) using MBS648888. ADM (arrow) was detected using MBS648888.
Rabbit Anti-ADM Polyclonal Antibody – Cat# MBS648888

Western Blot (WB) of anti-ADM antibody

Western Blot analysis in MCF-7 whole cell lysate (20ug) using MBS648888 (1:2000). Secondary Goat Anti-Rabbit IgG, (H+L), peroxidase conjugated at 1:10,000. Predicted band size: 20kD. Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-ADM Polyclonal Antibody, Western Blot (WB) – Cat# MBS648888

Flow Cytometry (FC/FACS) of anti-ADM antibody

Flow Cytometric analysis is MDA-MB435 cells using MBS648888 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-ADM Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS648888

Testing Data of anti-ADM antibody

Overlay histogram showing A549 cells stained with MBS648888 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% BSA to block non-specific protein-protein interactions followed by MBS648888 (1:25) for 60 mins at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1:400 for 40 mins at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10e6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Rabbit Anti-ADM Polyclonal Antibody, Testing Data – Cat# MBS648888

Other Notes

Small volumes of anti-ADM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ADM purchase

MBS648888 is an antibody targeting ADM. Also known as ADM, ID (ADM, AM, Adrenomedullin, Proadrenomedullin N-20 terminal peptide, ProAM N-terminal 20 peptide). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ADM. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ADM
NCBI Official Symbol
ADM
UniProt Protein Name
ADM
UniProt Synonym Protein Names
ProAM N-terminal 20 peptide
Protein Family
UniProt Gene Name
ADM
UniProt Synonym Gene Names
AM; AM; PAMP; ProAM-N20
UniProt Entry Name
ADML_PIG
NCBI Protein Information
ADM; pro-adrenomedullin

UniProt Comments for ADM

Function: AM and PAMP are potent hypotensive and vasodilatator agents.

Subcellular location: Secreted.

Tissue specificity: Highly expressed in adrenal glands, lung and kidney.

Sequence similarities: Belongs to the adrenomedullin family.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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