Fluorescent confocal image of HeLa cells stained with MBS648905. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP2745d LGR5 (loop2) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).
Western Blot analysis using MBS648905 in HepG2 cell line lysates (35ug/lane). This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow).
Western Blot analysis of MBS648905 in mouse cerebellum tissue lysates (35ug/lane). LGR5/GPR49(arrow) was detected using the purified Pab.
Flow cytometric analysis of HepG2 cells using MBS648905 (bottom histogram) compared to a negative control cell (top histogram). FIRC-conjugated goat anti-rabbit secondary antibodies were used for the analysis.
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