Rabbit Anti-BECN1 Polyclonal Antibody – Cat# MBS648968

Dot blot analysis of beclin 1 Antibody (Phospho S64) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Beclin 1 (BECN1) Antibody – Rabbit Polyclonal

Name: BECN1 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS648968
Price: 895 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS648968

Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).[supplied by OMIM]. More details

$895 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Beclin 1 (BECN1), Polyclonal Antibody

Full Product Name

Beclin 1, phosphorylated (S64) (BECN1, GT197, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197)

Product Gene Name

anti-BECN1 antibody

Product Synonym Names

Anti -Beclin 1, phosphorylated (S64) (BECN1, GT197, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Human

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

51,896 Da

Chromosome Location

Chromosome: 17; NC_000017.10 (40962150..40976310, complement). Location: 17q21

OMIM

604378

3D Structure

ModBase 3D Structure for Q14457

Immunogen

beclin 1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S64 of human beclin 1.

Product Categories/Family for anti-BECN1 antibody

Antibodies; Abs to Autophagy

Applications Tested/Suitable for anti-BECN1 antibody

ELISA (EL/EIA), Gel Shift Assay (GS/EMSA)

Application Notes for anti-BECN1 antibody

Suitable for use in Dot Blot, ELISA
Dilution: ELISA: 1:1,000
Dot blot 1:500

Other Notes

Small volumes of anti-BECN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BECN1 purchase

MBS648968 is an antibody targeting BECN1. Also known as Beclin 1, phosphorylated (S64) (BECN1, GT197, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BECN1. It may not necessarily be applicable to this product.

NCBI GI #

4680381

NCBI GeneID

8678

NCBI Accession #

AAD27650.1

UniProt Primary Accession #

Q14457

UniProt Secondary Accession #

O75595; Q9UNA8

UniProt Related Accession #

Q14457

NCBI Official Full Name

beclin 1

NCBI Official Synonym Full Names

beclin 1, autophagy related

NCBI Official Symbol

BECN1

NCBI Official Synonym Symbols

ATG6; VPS30; beclin1

UniProt Protein Name

Beclin-1

UniProt Synonym Protein Names

Coiled-coil myosin-like BCL2-interacting protein; Protein GT197

Protein Family

Beclin

UniProt Gene Name

BECN1

UniProt Synonym Gene Names

GT197

UniProt Entry Name

BECN1_HUMAN

NCBI Protein Information

beclin-1; ATG6 autophagy related 6 homolog; coiled-coil myosin-like BCL2-interacting protein; beclin 1 (coiled-coil, moesin-like BCL2 interacting protein); beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)

NCBI Summary for BECN1

Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).[supplied by OMIM, Jul 2010]

UniProt Comments for BECN1

Function: Plays a central role in autophagy. Required for the abcission step in cytokinesis. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. Ref.12

Subunit structure: Interacts with GOPC and GRID2. Interacts with AMBRA1. Forms a complex with PIK3C3 and PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. UVRAG and ATG14 form mutually exclusive complexes through direct competition for BECN1-binding. The complex containing ATG14 up-regulates autophagy, while the one containing Rubicon down-regulates autophagy

By similarity. Interacts with BCL2 and BCL2L1. Interacts with VMP1. Interacts with PIK3CB

By similarity. Interacts with DAPK1. Interacts with human cytomegalovirus/HHV-5 protein TRS1. Ref.1 Ref.5 Ref.7 Ref.8 Ref.9 Ref.11 Ref.13 Ref.14

Subcellular location: Golgi apparatus › trans-Golgi network membrane; Peripheral membrane protein. Note: Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells

By similarity. Ref.8

Tissue specificity: Ubiquitous.

Post-translational modification: Phosphorylation at Thr-119 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy.Ubiquitinated. Deubiquitinated by USP10 and USP13, leading to stabilize the PIK3C3/VPS34-containing complexes. Ref.13

Sequence similarities: Belongs to the beclin family.

Research Articles on BECN1

1. Findings suggest an association between lapatinib-induced autophagy and the disruption of Her2-Beclin-1 complex.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BECN1 antibody

Reference Product	PubMed Publications
BCL2L1 antibody	>41 publications with BECN1 and BCL2L1
UVRAG antibody	>32 publications with BECN1 and UVRAG
BCL2 antibody	>28 publications with BECN1 and BCL2
ATG14 antibody	>17 publications with BECN1 and ATG14
AMBRA1 antibody	>14 publications with BECN1 and AMBRA1
PIK3C3 antibody	>6 publications with BECN1 and PIK3C3
PIK3R4 antibody	>5 publications with BECN1 and PIK3R4
DAPK1 antibody	>1 publications with BECN1 and DAPK1

Pathways associated with anti-BECN1 antibody

Products by Pathway	Pathway Diagram
Regulation Of Autophagy Pathway antibodies	Regulation Of Autophagy Pathway Diagram
Regulation Of Autophagy Pathway antibodies	Regulation Of Autophagy Pathway Diagram
Senescence And Autophagy Pathway antibodies	Senescence And Autophagy Pathway Diagram

Diseases associated with anti-BECN1 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>306 publications with BECN1 and Neoplasms
Nervous System Diseases Antibodies	>78 publications with BECN1 and Nervous System Diseases
Breast Neoplasms Antibodies	>51 publications with BECN1 and Breast Neoplasms
Necrosis Antibodies	>43 publications with BECN1 and Necrosis
Liver Diseases Antibodies	>38 publications with BECN1 and Liver Diseases
Cardiovascular Diseases Antibodies	>34 publications with BECN1 and Cardiovascular Diseases
Carcinoma, Hepatocellular Antibodies	>32 publications with BECN1 and Carcinoma, Hepatocellular
Inflammation Antibodies	>25 publications with BECN1 and Inflammation
Ovarian Neoplasms Antibodies	>21 publications with BECN1 and Ovarian Neoplasms
Neoplasms, Experimental Antibodies	>18 publications with BECN1 and Neoplasms, Experimental

Organs/Tissues associated with anti-BECN1 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>67 publications with BECN1 and Brain
Liver Antibodies	>52 publications with BECN1 and Liver
Blood Antibodies	>41 publications with BECN1 and Blood
Muscle Antibodies	>33 publications with BECN1 and Muscle
Lung Antibodies	>28 publications with BECN1 and Lung
Heart Antibodies	>27 publications with BECN1 and Heart
Vascular Antibodies	>26 publications with BECN1 and Vascular
Nerve Antibodies	>24 publications with BECN1 and Nerve
Prostate Antibodies	>20 publications with BECN1 and Prostate
Lymph Antibodies	>19 publications with BECN1 and Lymph