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Nitrotyrosine (nTyr) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6490091

MBS6490091 is an antibody targeting nTyr. Also known as Nitrotyrosine (nTyr) (FITC). Reactive in Human, Mouse. Host: Rabbit. More details

$1340 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Nitrotyrosine (nTyr), Polyclonal Antibody
Full Product Name
Nitrotyrosine (nTyr) (FITC)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes nitrated tyrosine residues. Species Crossreactivity: human, mouse
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Immunogen
Nitrated KLH
Conjugate
FITC
Positive Control
Tyrosine residues can be nitrated directly in situ on tissue sections using peroxynitrite to create a positive control. After the tissue section is deparaffinized, the slide is covered with a drop of PBS, pH 7.4 into which 10ul of peroxynitrite solution are mixed. Care should be taken that the solution maintains a pH of 7.4.
Note
Preservative Free

Product Categories/Family for anti-nTyr antibody

Applications Tested/Suitable for anti-nTyr antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-nTyr antibody
IHC-P: 1:50-1:500 mouse brain (animal treated with Zymosan) with pretreatment with citrate buffer, pH 6.0.
WB: 1:100-1:1000 when tested against nitrated BSA and nitrated ovalbumin.
Applications are based on unconjugated antibody.

Testing Data of anti-nTyr antibody

Nytrotyrosine staining of Mouse Brain (Zymosan), tissue pretreated with citrate buffer, pH 6.0. A 1:500 dilution of MBS612511 was used followed by HRP/DAB staining. Immunoreactivity is seen in the hippocampus.
Rabbit Anti-nTyr Polyclonal Antibody – Cat# MBS6490091

Immunohistochemistry (IHC) of anti-nTyr antibody

Negative control staining of normal cerebellum, tissue pretreated with citrate buffer, pH 6.0, Negative Control Reagent, IHC Select detection withMBS612511. No signal detected.
Rabbit Anti-nTyr Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6490091

Immunohistochemistry (IHC) of anti-nTyr antibody

Nitrotyrosine staining of polyclonal normal cerebellum, tissue pretreated with citrate buffer, pH 6.0, pAb at 1:500 overnight incubation, IHC Select detection withMBS612511. Immunoreactivity is seen in the neurons
Rabbit Anti-nTyr Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6490091

Immunohistochemistry (IHC) of anti-nTyr antibody

Negative control staining of normal cerebellum, tissue pretreated with citrate buffer, pH 6.0, Negative Control Reagent, IHC Select detection with MBS612511 No signal detected.
Rabbit Anti-nTyr Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6490091

Other Notes

Small volumes of anti-nTyr antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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