• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

PPM1F Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649035

Dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis. More details

$970 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PPM1F, Polyclonal Antibody
Full Product Name
PPM1F (Protein Phosphatase 1F, Ca(2+)/Calmodulin-dependent Protein Kinase Phosphatase, CaM-kinase Phosphatase, CaMKPase, Partner of PIX 2, Protein fem-2 Homolog, hFem-2, KIAA0015, POPX2)
Product Gene Name
anti-PPM1F antibody
Product Synonym Names
Anti -PPM1F (Protein Phosphatase 1F, Ca(2+)/Calmodulin-dependent Protein Kinase Phosphatase, CaM-kinase Phosphatase, CaMKPase, Partner of PIX 2, Protein fem-2 Homolog, hFem-2, KIAA0015, POPX2)
Immunogen Sequence
MSSGAPQKSS PMASGAEETP GFLDTLLQDF PALLNPEDPL PWKAPGTVLS QEEVEGELAE LAMGFLGSRK APPPLAAALA HEAVSQLLQT DLSEFRKLPR EEEEEEEDDD EEEKAPVTLL DAQSLAQSFF NRLWEVAGQW QKQVPLAARA SQRQWLVSIH AIRNTRRKME DRHVSLPSFN QLFGLSDPVN RAYFAVFDGH GGVDAARYAA VHVHTNAARQ PELPTDPEGA LREAFRRTDQ MFLRKAKRER LQSGTTGVCA LIAGATLHVA WLGDSQVILV QQGQVVKLME PHRPERQDEK ARIEALGGFV SHMDCWRVNG TLAVSRAIGD VFQKPYVSGE ADAASRALTG SEDYLLLACD GFFDVVPHQE VVGLVQSHLT RQQGSGLRVA EELVAAARER GSHDNITVMV VFLRDPQELL EGGNQGEGDP QAEGRRQDLP SSLPEPETQA PPRS
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid.
Purity/Purification
Serum
Serum
Specificity
Recognizes human PPM1F.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
49,831 Da
Chromosome Location
Chromosome: 22; NC_000022.10 (22273792..22307250, complement). Location: 22q11.22
3D Structure
ModBase 3D Structure for P49593
Immunogen
Full length human PPM1F, aa1-454 (NP_055449.1).

Product Categories/Family for anti-PPM1F antibody

Antibodies; Abs to Protein Phosphatases
Applications Tested/Suitable for anti-PPM1F antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-PPM1F antibody
Suitable for use in Immunoprecipitation and Western Blot.

Western Blot (WB) of anti-PPM1F antibody

Western Blot analysis of PPM1F expression in transfected 293T cell line by PPM1F polyclonal antibody. Lane 1: PPM1F transfected lysate (49.8kD). Lane 2: Non-transfected lysate.
Rabbit Anti-PPM1F Polyclonal Antibody – Cat# MBS649035

Immunoprecipitation (IP) of anti-PPM1F antibody

Immunoprecipitation of PPM1F transfected lysate using PPM1F rabbit polyclonal antibody and Protein A Magnetic Bead and immunoblotted with PPM1F mouse polyclonal antibody
Rabbit Anti-PPM1F Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS649035

Other Notes

Small volumes of anti-PPM1F antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PPM1F purchase

MBS649035 is an antibody targeting PPM1F. Also known as PPM1F (Protein Phosphatase 1F, Ca(2+)/Calmodulin-dependent Protein Kinase Phosphatase, CaM-kinase Phosphatase, CaMKPase, Partner of PIX 2, Protein fem-2 Homolog, hFem-2, KIAA0015, POPX2). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PPM1F. It may not necessarily be applicable to this product.

NCBI GI #
7661862
NCBI GeneID
9647
NCBI Accession #
NP_055449.1
UniProt Primary Accession #
P49593
UniProt Secondary Accession #
Q96PM2; A8K6G3
UniProt Related Accession #
P49593
GenBank Nucleotide #
NM_014634.3
NCBI Official Full Name
protein phosphatase 1F
NCBI Official Synonym Full Names
protein phosphatase, Mg2+/Mn2+ dependent, 1F
NCBI Official Symbol
PPM1F
NCBI Official Synonym Symbols
CAMKP; FEM-2; POPX2; hFEM-2; CaMKPase
UniProt Protein Name
Protein phosphatase 1F
UniProt Synonym Protein Names
Ca(2+)/calmodulin-dependent protein kinase phosphatase; CaM-kinase phosphatase; CaMKPase; Partner of PIX 2; Protein fem-2 homolog
Protein Family
Protein phosphatase
UniProt Gene Name
PPM1F
UniProt Synonym Gene Names
KIAA0015; POPX2; CaM-kinase phosphatase; CaMKPase; hFem-2
UniProt Entry Name
PPM1F_HUMAN
NCBI Protein Information
protein phosphatase 1F; PP2C phosphatase; partner of PIX 2; protein fem-2 homolog; CaM-kinase phosphatase; protein phosphatase 1F (PP2C domain containing); Ca(2+)/calmodulin-dependent protein kinase phosphatase

NCBI Summary for PPM1F

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for PPM1F

Function: Dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis.

Catalytic activity: A phosphoprotein + H2O = a protein + phosphate.

Cofactor: Binds 2 magnesium or manganese ions per subunit

By similarity.

Subunit structure: Associates with FEM1B.

Sequence similarities: Belongs to the PP2C family.

Sequence caution: The sequence BAA02803.2 differs from that shown. Reason: Erroneous initiation.
Show more

Research Articles on PPM1F

1. hCaMKP activity is reversibly regulated by oxidation/reduction at Cys-359

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-PPM1F antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >4 publications with PPM1F and Neoplasms
 Skin Diseases Antibodies  >1 publications with PPM1F and Skin Diseases

Organs/Tissues associated with anti-PPM1F antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >1 publications with PPM1F and Kidney
 Brain Antibodies  >1 publications with PPM1F and Brain
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000