• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

NRAS Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6490561

MBS6490561 is an antibody targeting NRAS. Also known as NRAS, NT (Neuroblastoma RAS Viral (v-ras) Oncogene Homolog, N-Ras, ALPS4, GTPase NRas, NS6, HRAS1, NRAS1, OTTHUMP00000013879, OTTMUSP00000023521, Transforming Protein N-Ras, v-Ras Neuroblastoma RAS Viral… More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NRAS, Polyclonal Antibody
Full Product Name
NRAS, NT (Neuroblastoma RAS Viral (v-ras) Oncogene Homolog, N-Ras, ALPS4, GTPase NRas, NS6, HRAS1, NRAS1, OTTHUMP00000013879, OTTMUSP00000023521, Transforming Protein N-Ras, v-Ras Neuroblastoma RAS Viral Oncogene Homolog) (PE)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Specificity
Recognizes human NRAS.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
Predicted: 21

Observed: 20
OMIM
164790
3D Structure
ModBase 3D Structure for P01111
Immunogen
Synthetic peptide selected from the N-terminal region of human NRAS (KLH).
Conjugate
PE
Note
Preservative Free

Product Categories/Family for anti-NRAS antibody

Applications Tested/Suitable for anti-NRAS antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-NRAS antibody
WB: 1:50-1:100
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-NRAS antibody

Western blot analysis of anti-NRAS Antibody (N-term) in CEM cell line lysates (35ug/lane). NRAS(arrow) was detected using the purified Pab.
Rabbit Anti-NRAS Polyclonal Antibody – Cat# MBS6490561

Western Blot (WB) of anti-NRAS antibody

Western blot analysis of NRAS (arrow) using rabbit polyclonal NRAS Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NRAS gene (Lane 2) (Origene Technologies).
Rabbit Anti-NRAS Polyclonal Antibody, Western Blot (WB) – Cat# MBS6490561

Flow Cytometry (FC/FACS) of anti-NRAS antibody

NRAS Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-NRAS Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6490561

Immunofluorescence (IF) of anti-NRAS antibody

Confocal immunofluorescent analysis of NRAS Antibody (N-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-NRAS Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6490561

Product References and Citations for anti-NRAS antibody

1. Smalley, K.S., Cancer Res. 68 (14), 5743-5752 (2008). 2. Banerji, U., Mol. Cancer Ther. 7(4), 737-739 (2008).

Other Notes

Small volumes of anti-NRAS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NRAS. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
GTPase NRas
NCBI Official Synonym Full Names
neuroblastoma RAS viral (v-ras) oncogene homolog
NCBI Official Symbol
NRAS
NCBI Official Synonym Symbols
NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
UniProt Protein Name
GTPase NRas
UniProt Synonym Protein Names
Transforming protein N-Ras
Protein Family
UniProt Gene Name
NRAS
UniProt Synonym Gene Names
HRAS1
UniProt Entry Name
RASN_HUMAN
NCBI Protein Information
GTPase NRas; N-ras protein part 4; transforming protein N-Ras; v-ras neuroblastoma RAS viral oncogene homolog

NCBI Summary for NRAS

This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000