• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Nucleoside Diphosphate Kinase D (NDPKD) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6490795

MBS6490795 is an antibody targeting NDPKD. Also known as Nucleoside Diphosphate Kinase D, NT (NDPKD, NDPK-D, NM23D, NM23H4, nm23-H4, NME4, Nucleoside Diphosphate Kinase Mitochondrial, NDP Kinase Mitochondrial, NDK) (Biotin). Reactive in Human. Host: Rabbit. More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Nucleoside Diphosphate Kinase D (NDPKD), Polyclonal Antibody
Full Product Name
Nucleoside Diphosphate Kinase D, NT (NDPKD, NDPK-D, NM23D, NM23H4, nm23-H4, NME4, Nucleoside Diphosphate Kinase Mitochondrial, NDP Kinase Mitochondrial, NDK) (Biotin)
Product Gene Name
anti-NDPKD antibody
Product Synonym Names
Non-metastatic Cells 4; Protein Expressed in; EC=2.7.4.6
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Specificity
Recognizes human NME4.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
12,979 Da
OMIM
601818
3D Structure
ModBase 3D Structure for O00746
Immunogen
Synthetic peptide selected from the N-terminal region of human NME4 (KLH).
Conjugate
Biotin
Note
Preservative Free

Product Categories/Family for anti-NDPKD antibody

Antibodies; Protein Kinases
Applications Tested/Suitable for anti-NDPKD antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-NDPKD antibody
IHC: 1:50-1:100
ELISA: 1:1,000
WB: 1:50-1:100
Applications are based on unconjugated antibody.

Immunohistochemistry (IHC) of anti-NDPKD antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-NDPKD Polyclonal Antibody – Cat# MBS6490795

Western Blot (WB) of anti-NDPKD antibody

Western blot analysis of NME4 (arrow) using rabbit polyclonal NME4 Antibody (M1). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NME4 gene.
Rabbit Anti-NDPKD Polyclonal Antibody, Western Blot (WB) – Cat# MBS6490795

Western Blot (WB) of anti-NDPKD antibody

Western blot analysis of NME4 (arrow) using rabbit polyclonal NME4 Antibody (M1). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NME4 gene.
Rabbit Anti-NDPKD Polyclonal Antibody, Western Blot (WB) – Cat# MBS6490795

Product References and Citations for anti-NDPKD antibody

1. Milon, L., et al., Hum. Genet. 99(4):550-557 (1997).

Other Notes

Small volumes of anti-NDPKD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NDPKD. It may not necessarily be applicable to this product.

NCBI GI #
556695376
NCBI GeneID
4833
NCBI Accession #
NP_001273362.1
UniProt Primary Accession #
O00746
UniProt Secondary Accession #
Q5U0M9; A2IDD0
UniProt Related Accession #
O00746
GenBank Nucleotide #
NM_001286433.1
NCBI Official Full Name
nucleoside diphosphate kinase, mitochondrial isoform b
NCBI Official Synonym Full Names
NME/NM23 nucleoside diphosphate kinase 4
NCBI Official Symbol
NME4
NCBI Official Synonym Symbols
NDPK-D; NM23H4; nm23-H4
UniProt Protein Name
Nucleoside diphosphate kinase, mitochondrial
UniProt Synonym Protein Names
Nucleoside diphosphate kinase D; NDPKD; nm23-H4
UniProt Gene Name
NME4
UniProt Synonym Gene Names
NM23D; NDK; NDP kinase, mitochondrial; NDPKD
UniProt Entry Name
NDKM_HUMAN
NCBI Protein Information
nucleoside diphosphate kinase, mitochondrial; NDK; NDP kinase D; NDP kinase, mitochondrial; NDPKD; non-metastatic cells 4, protein expressed in; nucleoside diphosphate kinase D

NCBI Summary for NDPKD

The nucleoside diphosphate (NDP) kinases (EC 2.7.4.6) are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4 (Milon et al., 1997 [PubMed 9099850]).[supplied by OMIM, May 2008]
Show more

UniProt Comments for NDPKD

NME4: Major role in the synthesis of nucleoside triphosphates other than ATP. Belongs to the NDK family.

Protein type: Kinase, other; Nucleotide Metabolism - purine; EC 2.7.4.6; Kinase, nucleoside diphosphate; Nucleotide Metabolism - pyrimidine; Mitochondrial; Other group; NDK family

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial intermembrane space; cytosol

Molecular Function: protein binding; metal ion binding; nucleoside diphosphate kinase activity; protein complex binding; ATP binding

Biological Process: regulation of apoptosis; GTP biosynthetic process; CTP biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleotide metabolic process; nucleobase, nucleoside and nucleotide interconversion; UTP biosynthetic process; nucleoside triphosphate biosynthetic process; nucleoside metabolic process; nucleoside diphosphate phosphorylation; purine nucleotide metabolic process
Show more

Research Articles on NDPKD

1. findings show NDPKs (NM23-H1/H2/H4) interact with and provide GTP to dynamins, allowing these motor proteins to work with high thermodynamic efficiency for membrane remodeling

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NDPKD antibody

 Reference Product  PubMed Publications
 AK1 antibody  >1 publications with NDPKD and AK1

Pathways associated with anti-NDPKD antibody

 Products by Pathway  Pathway Diagram
 Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway antibodies  Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway Diagram
 Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway antibodies  Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway Diagram
 Guanine Ribonucleotide Biosynthesis IMP => GDP,GTP Pathway antibodies  Guanine Ribonucleotide Biosynthesis IMP => GDP,GTP Pathway Diagram
 Guanine Ribonucleotide Biosynthesis IMP => GDP,GTP Pathway antibodies  Guanine Ribonucleotide Biosynthesis IMP => GDP,GTP Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Nucleotides Pathway antibodies  Metabolism Of Nucleotides Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Pyrimidine Metabolism Pathway antibodies  Pyrimidine Metabolism Pathway Diagram

Diseases associated with anti-NDPKD antibody

 Disease Name  Pubmed Publications
 Lung Diseases Antibodies  >1 publications with NDPKD and Lung Diseases
 Nervous System Diseases Antibodies  >1 publications with NDPKD and Nervous System Diseases
 Neoplasm Metastasis Antibodies  >1 publications with NDPKD and Neoplasm Metastasis
 Cardiomyopathies Antibodies  >1 publications with NDPKD and Cardiomyopathies
 Cardiovascular Diseases Antibodies  >1 publications with NDPKD and Cardiovascular Diseases
 Breast Neoplasms Antibodies  >1 publications with NDPKD and Breast Neoplasms
 Anemia Antibodies  >1 publications with NDPKD and Anemia
 Inflammation Antibodies  >1 publications with NDPKD and Inflammation
 Kidney Diseases Antibodies  >1 publications with NDPKD and Kidney Diseases

Organs/Tissues associated with anti-NDPKD antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >3 publications with NDPKD and Liver
 Stomach Antibodies  >3 publications with NDPKD and Stomach
 Kidney Antibodies  >2 publications with NDPKD and Kidney
 Blood Antibodies  >2 publications with NDPKD and Blood
 Muscle Antibodies  >2 publications with NDPKD and Muscle
 Lymph Node Antibodies  >2 publications with NDPKD and Lymph Node
 Heart Antibodies  >2 publications with NDPKD and Heart
 Ovary Antibodies  >1 publications with NDPKD and Ovary
 Intestine Antibodies  >1 publications with NDPKD and Intestine
 Embryonic Tissue Antibodies  >1 publications with NDPKD and Embryonic Tissue
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000