Mouse Anti-RHOT1 Polyclonal Antibody – Cat# MBS649092

Western Blot analysis of RHOT1 expression in transfected 293T cell line by RHOT1 polyclonal antibody. Lane 1: RHOT1 transfected lysate (72.49kD). Lane 2: Non-transfected lysate.

RHOT1 Antibody – Mouse Polyclonal

Name: RHOT1 Antibody; Polyclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS649092
Price: 880 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649092

MBS649092 is an antibody targeting RHOT1. Also known as RHOT1 (Ras Homolog Gene Family Member T1, ARHT1, Mitochondrial Rho GTPase 1, MIRO1, MIRO-1, hMiro-1, Rac-GTP-binding Protein-like Protein). Reactive in Human. Host: Mouse. More details

$880 / 0.05 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

RHOT1, Polyclonal Antibody

Full Product Name

RHOT1 (Ras Homolog Gene Family Member T1, ARHT1, Mitochondrial Rho GTPase 1, MIRO1, MIRO-1, hMiro-1, Rac-GTP-binding Protein-like Protein)

Product Gene Name

anti-RHOT1 antibody

Product Synonym Names

Anti -RHOT1 (Ras Homolog Gene Family Member T1, ARHT1, Mitochondrial Rho GTPase 1, MIRO1, MIRO-1, hMiro-1, Rac-GTP-binding Protein-like Protein)

Immunogen Sequence

MKKDVRILLV GEPRVGKTSL IMSLVSEEFP EEVPPRAEEI TIPADVTPER VPTHIVDYSE AEQSDEQLHQ EISQANVICI VYAVNNKHSI DKVTSRWIPL INERTDKDSR LPLILVGNKS DLVEYSSMET ILPIMNQYTE IETCVECSAK NLKNISELFY YAQKAVLHPT GPLYCPEEKE MKPACIKALT RIFKISDQDN DGTLNDAELN FFQRICFNTP LAPQALEDVK NVVRKHISDG VADSGLTLKG FLFLHTLFIQ RGRHETTWTV LRRFGYDDDL DLTPEYLFPL LKIPPDCTTE LNHHAYLFLQ STFDKHDLDR DCALSPDELK DLFKVFPYIP WGPDVNNTVC TNERGWITYQ GFLSQWTLTT YLDVQRCLEY LGYLGYSILT EQESQASAVT VTRDKKIDLQ KKQTQRNVFR CNVIGVKNCG KSGVLQALLG RNLMRQKKIR EDHKSYYAIN TVYVYGQEKY LLLHDISESE FLTEAEIICD VVCLVYDVSN PKSFEYCARI FKQHFMDSRI PCLIVAAKSD LHEVKQEYSI SPTDFCRKHK MPPPQAFTCN TADAPSKDIF VKLTTMAMYP HARLRCMCTC NRCTFCICQN FLNSDLLQSV KNKIFTAVLN RHVTQADLKS STFWLRASFG ATVFAVLGFA MYKALLKQR

Clonality

Polyclonal

Isotype

IgG

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Recognizes human RHOT1.

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

70,784 Da

Chromosome Location

Chromosome: 17; NC_000017.10 (30469473..30552746). Location: 17q11.2

OMIM

613888

3D Structure

ModBase 3D Structure for Q8IXI2

Immunogen

Full length human RHOT1, aa1-659 (NP_001028738.1).

Product Categories/Family for anti-RHOT1 antibody

Antibodies; Abs to GTPase; Rab; Ras; Rho Proteins

Applications Tested/Suitable for anti-RHOT1 antibody

Western Blot (WB)

Application Notes for anti-RHOT1 antibody

Suitable for use in Western Blot.

Other Notes

Small volumes of anti-RHOT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RHOT1. It may not necessarily be applicable to this product.

NCBI GI #

46249998

NCBI GeneID

55288

NCBI Accession #

AAH68463.1

UniProt Primary Accession #

Q8IXI2

UniProt Secondary Accession #

Q6NUR3; Q6P9F8; Q6PJG1; Q6YMW8; Q86UB0; Q8IW28; Q8IXJ7; A4FVB6; A6NFV0; B4DG48; J9JIH9

UniProt Related Accession #

Q8IXI2

NCBI Official Full Name

RHOT1 protein, partial

NCBI Official Synonym Full Names

ras homolog family member T1

NCBI Official Symbol

RHOT1

NCBI Official Synonym Symbols

ARHT1; MIRO1; MIRO-1

UniProt Protein Name

Mitochondrial Rho GTPase 1

UniProt Synonym Protein Names

Rac-GTP-binding protein-like protein; Ras homolog gene family member T1

UniProt Gene Name

RHOT1

UniProt Synonym Gene Names

ARHT1; MIRO-1; hMiro-1

UniProt Entry Name

MIRO1_HUMAN

NCBI Protein Information

mitochondrial Rho GTPase 1; hMiro-1; mitochondrial Rho 1; mitochondrial Rho (MIRO) GTPase 1; ras homolog gene family, member T1; rac-GTP binding protein-like protein; rac-GTP-binding protein-like protein

UniProt Comments for RHOT1

Function: Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution. Ref.2 Ref.9

Subunit structure: Interacts with the kinesin-binding proteins TRAK1/OIP106 and TRAK2/GRIF1, forming a link between mitochondria and the trafficking apparatus of the microtubules. Ref.9

Subcellular location: Mitochondrion outer membrane; Single-pass type IV membrane protein. Note: Colocalizes with MGARP and RHOT2 at the mitochondria. Ref.2 Ref.10

Tissue specificity: Ubiquitously expressed. Expressed at high level in heart and skeletal muscle. Ref.2

Sequence similarities: Belongs to the mitochondrial Rho GTPase family.Contains 2 EF-hand domains.Contains 2 Miro domains.

Sequence caution: The sequence AAH41114.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH68463.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA91969.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB14185.1 differs from that shown. Reason: Erroneous initiation.

Research Articles on RHOT1

1. results indicated that the low-expression levels of RhoT1 and Smad4 were significantly associated with LNM and shorter survival. RhoT1 may be considered as a potential novel marker for predicting the outcome in patients with pancreatic cancer

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-RHOT1 antibody

Products by Pathway	Pathway Diagram
Rho GTPase Cycle Pathway antibodies	Rho GTPase Cycle Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By Rho GTPases Pathway antibodies	Signaling By Rho GTPases Pathway Diagram

Diseases associated with anti-RHOT1 antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>3 publications with RHOT1 and Nervous System Diseases
Neoplasms Antibodies	>1 publications with RHOT1 and Neoplasms

Organs/Tissues associated with anti-RHOT1 antibody

Organ/Tissue Name	Pubmed Publications
Nerve Antibodies	>5 publications with RHOT1 and Nerve
Brain Antibodies	>2 publications with RHOT1 and Brain
Ganglia Antibodies	>1 publications with RHOT1 and Ganglia
Bone Antibodies	>1 publications with RHOT1 and Bone
Lymph Node Antibodies	>1 publications with RHOT1 and Lymph Node
Kidney Antibodies	>1 publications with RHOT1 and Kidney