• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

OAT Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6490946

MBS6490946 is an antibody targeting OAT. Also known as OAT, NT (Ornithine Aminotransferase, Ornithine delta-Aminotransferase, Ornithine-oxo-acid Aminotransferase, DKFZp781A11155, GACR, HOGA, OATASE, OKT) (PE). Reactive in Human. Host: Rabbit. More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
OAT, Polyclonal Antibody
Full Product Name
OAT, NT (Ornithine Aminotransferase, Ornithine delta-Aminotransferase, Ornithine-oxo-acid Aminotransferase, DKFZp781A11155, GACR, HOGA, OATASE, OKT) (PE)
Product Gene Name
anti-OAT antibody
Product Synonym Names
EC=2.6.1.13; Ornithine Aminotransferase Hepatic Form; Ornithine Aminotransferase Mitochondrial; Ornithine Aminotransferase Renal Form
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Immunoaffinity chromatography.
Specificity
Recognizes human OAT.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
48,535 Da
Chromosome Location
Chromosome: 10; NC_000010.10 (126085872..126107545, complement). Location: 10q26
OMIM
258870
3D Structure
ModBase 3D Structure for P04181
Immunogen
Synthetic peptide selected from the N-terminal region of human OAT (KLH).
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-OAT antibody

Antibodies; Enzymes; Aminotransferase
Applications Tested/Suitable for anti-OAT antibody
Western Blot (WB), FLISA
Application Notes for anti-OAT antibody
WB: 1:100-1:500
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Immunofluorescence (IF) of anti-OAT antibody

Fluorescent image of A549 cell stained with OAT Antibody (N-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with OAT primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).OAT immunoreactivity is localized to Mitochondrion significantly.
Rabbit Anti-OAT Polyclonal Antibody – Cat# MBS6490946

Western Blot (WB) of anti-OAT antibody

Western blot analysis of OAT Antibody (N-term) in 293(lane 1), MDA-MB231 cell line(lane 2) lysates (35ug/lane). OAT (arrow) was detected using the purified Pab.
Rabbit Anti-OAT Polyclonal Antibody, Western Blot (WB) – Cat# MBS6490946

Western Blot (WB) of anti-OAT antibody

Western blot analysis of OAT (arrow) using rabbit polyclonal OAT Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the OAT gene.
Rabbit Anti-OAT Polyclonal Antibody, Western Blot (WB) – Cat# MBS6490946

Immunohistochemistry (IHC) of anti-OAT antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with OAT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-OAT Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6490946

Immunohistochemistry (IHC) of anti-OAT antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with OAT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-OAT Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6490946

Product References and Citations for anti-OAT antibody

1. Michaud J., et.al., Am. J. Hum. Genet. 56:616-622(1995).

Other Notes

Small volumes of anti-OAT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for OAT. It may not necessarily be applicable to this product.

NCBI GI #
4557809
NCBI GeneID
4942
NCBI Accession #
NP_000265.1
UniProt Primary Accession #
P04181
UniProt Secondary Accession #
Q16068; Q16069; Q68CS0; Q6IAV9; Q9UD03; D3DRF0
UniProt Related Accession #
P04181
GenBank Nucleotide #
NM_000274.3
NCBI Official Full Name
ornithine aminotransferase, mitochondrial isoform 1
NCBI Official Synonym Full Names
ornithine aminotransferase
NCBI Official Symbol
OAT
NCBI Official Synonym Symbols
OKT; GACR; HOGA; OATASE
UniProt Protein Name
Ornithine aminotransferase, mitochondrial
UniProt Synonym Protein Names
Ornithine delta-aminotransferase; Ornithine--oxo-acid aminotransferase
Protein Family
Ornithine aminotransferase
UniProt Gene Name
OAT
UniProt Entry Name
OAT_HUMAN
NCBI Protein Information
ornithine aminotransferase, mitochondrial; gyrate atrophy; ornithine delta-aminotransferase; ornithine-oxo-acid aminotransferase

NCBI Summary for OAT

This gene encodes the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Alternatively spliced transcript variants encoding different isoforms have been described. Related pseudogenes have been defined on the X chromosome. [provided by RefSeq, Jan 2010]
Show more

UniProt Comments for OAT

OAT: Defects in OAT are the cause of hyperornithinemia with gyrate atrophy of choroid and retina (HOGA). HOGA is a slowly progressive blinding autosomal recessive disorder. Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; Amino Acid Metabolism - arginine and proline; EC 2.6.1.13; Mitochondrial

Chromosomal Location of Human Ortholog: 10q26

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: ornithine-oxo-acid transaminase activity; pyridoxal phosphate binding

Biological Process: visual perception; amino acid biosynthetic process

Disease: Gyrate Atrophy Of Choroid And Retina
Show more

Research Articles on OAT

1. OAT was a highly homologous and stable protein located in the mitochondria.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-OAT antibody

 Products by Pathway  Pathway Diagram
 Amino Acid Synthesis And Interconversion (transamination) Pathway antibodies  Amino Acid Synthesis And Interconversion (transamination) Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
 Urea Cycle And Metabolism Of Amino Groups Pathway antibodies  Urea Cycle And Metabolism Of Amino Groups Pathway Diagram
 Arginine Biosynthesis IV Pathway antibodies  Arginine Biosynthesis IV Pathway Diagram
 Arginine Biosynthesis IV Pathway antibodies  Arginine Biosynthesis IV Pathway Diagram
 Arginine Degradation I (arginase Pathway) antibodies  Arginine Degradation I (arginase Pathway) Diagram
 Arginine Degradation I (arginase Pathway) antibodies  Arginine Degradation I (arginase Pathway) Diagram

Diseases associated with anti-OAT antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1249 publications with OAT and Neoplasms
 Carcinoma Antibodies  >1133 publications with OAT and Carcinoma
 Lung Neoplasms Antibodies  >877 publications with OAT and Lung Neoplasms
 Adenocarcinoma Antibodies  >349 publications with OAT and Adenocarcinoma
 Carcinoma, Squamous Cell Antibodies  >285 publications with OAT and Carcinoma, Squamous Cell
 Cardiovascular Diseases Antibodies  >250 publications with OAT and Cardiovascular Diseases
 Neoplasm Metastasis Antibodies  >206 publications with OAT and Neoplasm Metastasis
 Drug Toxicity Antibodies  >157 publications with OAT and Drug Toxicity
 Heart Diseases Antibodies  >110 publications with OAT and Heart Diseases
 Hyperlipidemias Antibodies  >96 publications with OAT and Hyperlipidemias

Organs/Tissues associated with anti-OAT antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >852 publications with OAT and Lung
 Blood Antibodies  >810 publications with OAT and Blood
 Liver Antibodies  >340 publications with OAT and Liver
 Intestine Antibodies  >272 publications with OAT and Intestine
 Kidney Antibodies  >267 publications with OAT and Kidney
 Brain Antibodies  >222 publications with OAT and Brain
 Heart Antibodies  >127 publications with OAT and Heart
 Bone Antibodies  >121 publications with OAT and Bone
 Vascular Antibodies  >112 publications with OAT and Vascular
 Skin Antibodies  >97 publications with OAT and Skin
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000