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PRPF19 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649107

PRPF19 is the human homolog of yeast Pso4, a gene essential for cell survival and DNA repair. More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PRPF19, Polyclonal Antibody
Full Product Name
PRPF19, NT (PRPF19, NMP200, PRP19, SNEV, Pre-mRNA-processing factor 19, Nuclear matrix protein 200, PRP19/PSO4 homolog, Senescence evasion factor)
Product Gene Name
anti-Prpf19 antibody
Product Synonym Names
Anti -PRPF19, NT (PRPF19, NMP200, PRP19, SNEV, Pre-mRNA-processing factor 19, Nuclear matrix protein 200, PRP19/PSO4 homolog, Senescence evasion factor)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human, mouse
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
55,239 Da
Chromosome Location
Chromosome: 19; NC_000085.6 (10895231..10909559). Location: 19 A; 19 7.33 cM
3D Structure
ModBase 3D Structure for Q99KP6
Immunogen
PRPF19 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acids 10-40 from the N-terminal region of human PRPF19.

Product Categories/Family for anti-Prpf19 antibody

Antibodies; Abs to Nuclear Proteins
Applications Tested/Suitable for anti-Prpf19 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-Prpf19 antibody
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-Prpf19 antibody

Western blot analysis of PRPF19 Antibody (N-term) in MCF-7 cell line lysates (35ug/lane). PRPF19 (arrow) was detected using the purified Pab.
Rabbit Anti-PRPF19 Polyclonal Antibody – Cat# MBS649107

Western Blot (WB) of anti-Prpf19 antibody

Western blot analysis of PRPF19 Antibody (N-term) in mouse brain tissue lysates (35ug/lane). PRPF19 (arrow) was detected using the purified Pab.
Rabbit Anti-PRPF19 Polyclonal Antibody, Western Blot (WB) – Cat# MBS649107

Immunohistochemistry (IHC) of anti-Prpf19 antibody

PRPF19 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PRPF19 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-PRPF19 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS649107

Flow Cytometry (FC/FACS) of anti-Prpf19 antibody

PRPF19 Antibody (N-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-PRPF19 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS649107

Other Notes

Small volumes of anti-Prpf19 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Prpf19 purchase

MBS649107 is an antibody targeting Prpf19. Also known as PRPF19, NT (PRPF19, NMP200, PRP19, SNEV, Pre-mRNA-processing factor 19, Nuclear matrix protein 200, PRP19/PSO4 homolog, Senescence evasion factor). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Prpf19. It may not necessarily be applicable to this product.

NCBI GI #
359718922
NCBI GeneID
28000
NCBI Accession #
NP_001240773.1
UniProt Primary Accession #
Q99KP6
UniProt Secondary Accession #
Q3TP64; Q8BKZ5; Q8BVQ4
UniProt Related Accession #
Q99KP6
GenBank Nucleotide #
NM_001253844.1
NCBI Official Full Name
pre-mRNA-processing factor 19 isoform 3
NCBI Official Synonym Full Names
PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae)
NCBI Official Symbol
Prpf19
NCBI Official Synonym Symbols
PSO4; Snev; Prp19; NMP200; AA617263; AL024362; D19Wsu55e
UniProt Protein Name
Pre-mRNA-processing factor 19
UniProt Synonym Protein Names
Nuclear matrix protein 200; PRP19/PSO4 homolog; Senescence evasion factor
UniProt Gene Name
Prpf19
UniProt Synonym Gene Names
Prp19; Snev
UniProt Entry Name
PRP19_MOUSE
NCBI Protein Information
pre-mRNA-processing factor 19; prp19 beta; PRP19/PSO4 homolog; senescence evasion factor; nuclear matrix protein 200; nuclear matrix protein SNEV

UniProt Comments for Prpf19

Function: Plays a role in DNA double-strand break (DSB) repair. Binds double-stranded DNA in a sequence-nonspecific manner. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing

By similarity.

Subunit structure: Homooligomer

By similarity. Identified in the spliceosome C complex. Component of the PSO4 complex, composed of PRPF19, CDC5L, PLRG1. Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts in the complex directly with CDC5L, PLRG1 and BCAS2. Interacts with APEX1, DNTT and PSMB4

By similarity. Interacts with CWC22 and EIF4A3 in an RNA-independent manner

By similarity.

Subcellular location: Nucleus

By similarity. Nucleus › nucleoplasm

By similarity. Cytoplasm › cytoskeleton › spindle

By similarity. Note: Nucleoplasmic in interphase cells. Irregularly distributed in anaphase cells. In prophase cells, uniformly distributed, but not associated with condensing chromosomes. Found in extrachromosomal regions in metaphase cells. Mainly localized to the mitotic spindle apparatus when chromosomes segregate during anaphase. When nuclei reform during late telophase, uniformly distributed in daughter cells and displays no preferred association with decondensing chromatin

By similarity.

Sequence similarities: Belongs to the WD repeat PRP19 family.Contains 1 U-box domain.Contains 7 WD repeats.
Show more

Research Articles on Prpf19

1. The knockdown of PRPF19 or EIF4A3 decreased splicing of Lhb, or of both beta subunit transcripts, respectively.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Prpf19 antibody

 Products by Pathway  Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram
 Spliceosome, 35S U5-snRNP Pathway antibodies  Spliceosome, 35S U5-snRNP Pathway Diagram
 Spliceosome, Prp19/CDC5L Complex Pathway antibodies  Spliceosome, Prp19/CDC5L Complex Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram

Diseases associated with anti-Prpf19 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >2 publications with Prpf19 and Carcinoma
 Cell Transformation, Neoplastic Antibodies  >1 publications with Prpf19 and Cell Transformation, Neoplastic
 Pancreatic Neoplasms Antibodies  >1 publications with Prpf19 and Pancreatic Neoplasms
 Disease Models, Animal Antibodies  >1 publications with Prpf19 and Disease Models, Animal
 Genomic Instability Antibodies  >1 publications with Prpf19 and Genomic Instability
 Carcinoma, Hepatocellular Antibodies  >1 publications with Prpf19 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >1 publications with Prpf19 and Liver Neoplasms

Organs/Tissues associated with anti-Prpf19 antibody

 Organ/Tissue Name  Pubmed Publications
 Pituitary Gland Antibodies  >1 publications with Prpf19 and Pituitary Gland
 Blood Antibodies  >1 publications with Prpf19 and Blood
 Heart Antibodies  >1 publications with Prpf19 and Heart
 Liver Antibodies  >1 publications with Prpf19 and Liver
 Joint Antibodies  >1 publications with Prpf19 and Joint
 Pancreas Antibodies  >1 publications with Prpf19 and Pancreas
 Brain Antibodies  >1 publications with Prpf19 and Brain
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