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PARK2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6492094

MBS6492094 is an antibody targeting PARK2. Also known as PARK2, NT (E3 Ubiquitin-protein Ligase Parkin, PRKN, Parkinson Juvenile Disease Protein 2, Parkinson Disease Protein 2) (APC). Reactive in Human. Host: Rabbit. More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PARK2, Polyclonal Antibody
Full Product Name
PARK2, NT (E3 Ubiquitin-protein Ligase Parkin, PRKN, Parkinson Juvenile Disease Protein 2, Parkinson Disease Protein 2) (APC)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Specificity
Recognizes human Park2.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
46,413 Da
Immunogen
Synthetic peptide in the C-terminal region of human Park2 (KLH).
Conjugate
APC
Note
Preservative Free

Product Categories/Family for anti-PARK2 antibody

Applications Tested/Suitable for anti-PARK2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-PARK2 antibody
WB: 1:100-1:500
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-PARK2 antibody

Western blot analysis of anti-PARK2 (Parkin) Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). PARK2 (Parkin)(arrow) was detected using the purified Pab.
Rabbit Anti-PARK2 Polyclonal Antibody – Cat# MBS6492094

Flow Cytometry (FC/FACS) of anti-PARK2 antibody

Parkin Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-PARK2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6492094

Immunofluorescence (IF) of anti-PARK2 antibody

Confocal immunofluorescent analysis of Parkin Antibody (N-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-PARK2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6492094

Immunohistochemistry (IHC) of anti-PARK2 antibody

Parkin Antibody (N-term) (AP6402a)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Parkin Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-PARK2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6492094

Product References and Citations for anti-PARK2 antibody

1. Kumru, H., et al., Ann. Neurol. 56(4):599-603 (2004). 2. Pigullo, S., et al., Parkinsonism Relat. Disord. 10(6):357-362 (2004). 3. Yao, D., et al., Proc. Natl. Acad. Sci. USA 101(29):10810-10814 (2004). 4. West, A.B., et al., J. Biol. Chem. 279(28):28896-28902 (2004). 5. Wang, F., et al., Genes Chromosomes Cancer 40(2):85-96 (2004).

Other Notes

Small volumes of anti-PARK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PARK2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
parkin isoform 1
NCBI Official Synonym Full Names
parkin RBR E3 ubiquitin protein ligase
NCBI Official Symbol
PARK2
NCBI Official Synonym Symbols
PDJ; PRKN; AR-JP; LPRS2
NCBI Protein Information
E3 ubiquitin-protein ligase parkin; parkinson juvenile disease protein 2; parkinson protein 2, E3 ubiquitin protein ligase (parkin); Parkinson disease (autosomal recessive, juvenile) 2, parkin

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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