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CYP26A1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649256

CYP26A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids.This endoplasmic reticulum… More details

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Product Overview

Online Datasheet in PDF
Product Name
CYP26A1, Polyclonal Antibody
Full Product Name
CYP26A1, CT (CYP26A1, CYP26, P450RAI1, Cytochrome P450 26A1, Cytochrome P450 retinoic acid-inactivating 1, Retinoic acid 4-hydroxylase, Retinoic acid-metabolizing cytochrome)
Product Gene Name
Product Synonym Names
Anti -CYP26A1, CT (CYP26A1, CYP26, P450RAI1, Cytochrome P450 26A1, Cytochrome P450 retinoic acid-inactivating 1, Retinoic acid 4-hydroxylase, Retinoic acid-metabolizing cytochrome)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Purified
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Specificity
Human, mouse
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
56,199 Da
Chromosome Location
Chromosome: 10; NC_000010.10 (94833232..94837641). Location: 10q23-q24
OMIM
602239
3D Structure
ModBase 3D Structure for O43174
Immunogen
CYP26A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-506 amino acids from the C-terminal region of human CYP26A1.
Applications Tested/Suitable for anti-CYP26A1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-CYP26A1 antibody
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-CYP26A1 antibody

Western blot analysis of CYP26A1 Antibody (C-term) in mouse NIH-3T3 cell lysates (35ug/lane).CYP26A1 (arrow) was detected using the purified Pab.
Rabbit Anti-CYP26A1 Polyclonal Antibody – Cat# MBS649256

Immunohistochemistry (IHC) of anti-CYP26A1 antibody

CYP26A1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CYP26A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-CYP26A1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS649256

Flow Cytometry (FC/FACS) of anti-CYP26A1 antibody

CYP26A1 Antibody (C-term) flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-CYP26A1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS649256

Other Notes

Small volumes of anti-CYP26A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CYP26A1 purchase

MBS649256 is an antibody targeting CYP26A1. Also known as CYP26A1, CT (CYP26A1, CYP26, P450RAI1, Cytochrome P450 26A1, Cytochrome P450 retinoic acid-inactivating 1, Retinoic acid 4-hydroxylase, Retinoic acid-metabolizing cytochrome). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CYP26A1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
cytochrome P450 26A1 isoform 1
NCBI Official Synonym Full Names
cytochrome P450, family 26, subfamily A, polypeptide 1
NCBI Official Symbol
CYP26A1
NCBI Official Synonym Symbols
CP26; CYP26; P450RAI; P450RAI1
UniProt Protein Name
Cytochrome P450 26A1
UniProt Synonym Protein Names
Cytochrome P450 retinoic acid-inactivating 1; Cytochrome P450RAI; hP450RAI; Retinoic acid 4-hydroxylase; Retinoic acid-metabolizing cytochrome
Protein Family
UniProt Gene Name
CYP26A1
UniProt Synonym Gene Names
CYP26; P450RAI1; Cytochrome P450RAI; hP450RAI
UniProt Entry Name
CP26A_HUMAN
NCBI Protein Information
cytochrome P450 26A1; hP450RAI; cytochrome P450RAI; retinoic acid 4-hydroxylase; P450, retinoic acid-inactivating, 1; retinoic acid-metabolizing cytochrome; cytochrome P450 retinoic acid-inactivating 1; cytochrome P450, subfamily XXVIA, polypeptide 1

NCBI Summary for CYP26A1

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein acts on retinoids, including all-trans-retinoic acid (RA), with both 4-hydroxylation and 18-hydroxylation activities. This enzyme regulates the cellular level of retinoic acid which is involved in regulation of gene expression in both embryonic and adult tissues. Two alternatively spliced transcript variants of this gene, which encode the distinct isoforms, have been reported. [provided by RefSeq, Jul 2008]
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UniProt Comments for CYP26A1

Function: Plays a key role in retinoic acid metabolism. Acts on retinoids, including all-trans-retinoic acid (RA) and its stereoisomer 9-cis-RA. Capable of both 4-hydroxylation and 18-hydroxylation. Responsible for generation of several hydroxylated forms of RA, including 4-OH-RA, 4-oxo-RA and 18-OH-RA.

Cofactor: Heme group

By similarity.

Subcellular location: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue specificity: Highest levels in adult liver, heart, pituitary gland, adrenal gland, placenta and regions of the brain. Ref.6

Induction: By retinoic acid.

Sequence similarities: Belongs to the cytochrome P450 family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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