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THG1L Antibody – Mouse Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649305

Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage. This step is essential for proper recognition of the tRNA and for the fidelity of protein synthesis. More details

$880 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
THG1L, Polyclonal Antibody
Full Product Name
THG1L (ICF45, Probable tRNA(His) Guanylyltransferase, Interphase Cytoplasmic Foci Protein 45, tRNA-histidine Guanylyltransferase, FLJ11601, FLJ20546)
Product Gene Name
Product Synonym Names
Anti -THG1L (ICF45, Probable tRNA(His) Guanylyltransferase, Interphase Cytoplasmic Foci Protein 45, tRNA-histidine Guanylyltransferase, FLJ11601, FLJ20546)
Immunogen Sequence
MWGACKVKVH DSLATISITL RRYLRLGATM AKSKFEYVRD FEADDTCLAH CWVVVRLDGR NFHRFAEKHN FAKPNDSRAL QLMTKCAQTV MEELEDIVIA YGQSDEYSFV FKRKTNWFKR RASKFMTHVA SQFASSYVFY WRDYFEDQPL LYPPGFDGRV VVYPSNQTLK DYLSWRQADC HINNLYNTVF WALIQQSGLT PVQAQGRLQG TLAADKNEIL FSEFNINYNN EPPMYRKGTV LIWQKVDEVM TKEIKLPTEM EGKKMAVTRT RTKPVPLHCD IIGDAFWKEH PEILDEDS
Clonality
Polyclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human ICF45.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
34,831 Da
Chromosome Location
Chromosome: 5; NC_000005.9 (157158323..157166982). Location: 5q33.3
3D Structure
ModBase 3D Structure for Q9NWX6
Immunogen
Full length human ICF45, aa1-298 (AAH23521.1).
Applications Tested/Suitable for anti-THG1L antibody
Western Blot (WB)
Application Notes for anti-THG1L antibody
Suitable for use in Western Blot.

Western Blot (WB) of anti-THG1L antibody

THG1L polyclonal antibody. Western Blot analysis of THG1L expression in A-431.
Mouse Anti-THG1L Polyclonal Antibody – Cat# MBS649305

Western Blot (WB) of anti-THG1L antibody

Western Blot analysis of THG1L expression in transfected 293T cell line by THG1L polyclonal antibody. Lane1: ICF45 transfected lysate (32.78kD). Lane2: Non-transfected lysate.
Mouse Anti-THG1L Polyclonal Antibody, Western Blot (WB) – Cat# MBS649305

Other Notes

Small volumes of anti-THG1L antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for THG1L purchase

MBS649305 is an antibody targeting THG1L. Also known as THG1L (ICF45, Probable tRNA(His) Guanylyltransferase, Interphase Cytoplasmic Foci Protein 45, tRNA-histidine Guanylyltransferase, FLJ11601, FLJ20546). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for THG1L. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
THG1L protein
NCBI Official Synonym Full Names
tRNA-histidine guanylyltransferase 1-like (S. cerevisiae)
NCBI Official Symbol
THG1L
NCBI Official Synonym Symbols
ICF45; IHG-1
UniProt Protein Name
Probable tRNA(His) guanylyltransferase
UniProt Synonym Protein Names
Interphase cytoplasmic foci protein 45; tRNA-histidine guanylyltransferase
UniProt Gene Name
THG1L
UniProt Synonym Gene Names
ICF45
UniProt Entry Name
THG1_HUMAN
NCBI Protein Information
probable tRNA(His) guanylyltransferase; probable tRNA(His) guanylyltransferase; induced by high glucose-1; interphase cytoplasmic foci protein 45

UniProt Comments for THG1L

Function: Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage. This step is essential for proper recognition of the tRNA and for the fidelity of protein synthesis. Ref.8

Catalytic activity: p-tRNA(His) + ATP + GTP = pppG-P-tRNA(His) + AMP + diphosphate.

Cofactor: Binds 2 magnesium ions per subunit. Ref.8

Subunit structure: Homotetramer. Ref.8

Subcellular location: Cytoplasm. Note: Found near the nuclear membrane. Ref.1

Tissue specificity: Expressed in many tissues. Ref.1

Sequence similarities: Belongs to the tRNA(His) guanylyltransferase family.

Sequence caution: The sequence AAH01523.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAH01852.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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