IFI30 Antibody – Rabbit Polyclonal

The protein encoded by this gene is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing. [provided by RefSeq, Jul 2008]

Function: Lysosomal thiol reductase that can reduce protein disulfide bonds. May facilitate the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing. Facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds

By similarity. Facilitates also MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation

By similarity.

Subunit structure: Dimer; disulfide-linked. Ref.1 Ref.8

Subcellular location: Secreted. Lysosome Ref.1 Ref.2.

Induction: Expressed constitutively in antigen-presenting cells and induced by IFN-gamma in other cell types. Ref.1

Post-translational modification: N-glycosylated. Sugar chains contain mannose-6-phosphate. Ref.2 Ref.8Synthesized as a 35 kDa precursor which is then processed into the mature 30 kDa form via cleavage of N-terminal and C-terminal propeptides. Processing of the precursor is mediated by multiple lysosomal proteases.

Miscellaneous: Both precursor form and mature form have thiol reductase activity.

Sequence similarities: Belongs to the GILT family.

Biophysicochemical propertiesKinetic parameters:Kinetic parameters shown are for mature enzyme.KM=1200 µM for F(ab')2 fragment when denatured Ref.2KM=10 µM for F(ab')2 fragmentpH dependence:Optimum pH is 4-5.

Sequence caution: The sequence AAA36105.1 differs from that shown. Reason: Erroneous termination at position 251. Translated as stop.The sequence AAA36105.1 differs from that shown. Reason: Frameshift at positions 146 and 174. The sequence AAA36105.1 differs from that shown. Reason: Chimeric cDNA. N-terminal sequence identical to a region of chromosome 11.The sequence AAF04618.1 differs from that shown. Reason: Chimeric cDNA. N-terminal sequence identical to a region of chromosome 11.