Rabbit Anti-C5AR2 Polyclonal Antibody – Cat# MBS649343

Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with GPR77 antibody at 10ug/ml followed by biotinylated goat rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

C5AR2 Antibody – Rabbit Polyclonal

Name: C5AR2 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS649343
Price: 910 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649343

GPR77 is an Anaphylatoxin Receptor. Binding to anaphylatoxins can result in increased triglyceride synthesis, although its precise role in cellular responses has not been determined. More details

$910 / 0.05 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

GPR77 (C5AR2), Polyclonal Antibody

Full Product Name

GPR77, CT (C5a Anaphylatoxin Chemotactic Receptor C5L2, G-protein Coupled Receptor 77, C5L2)

Product Gene Name

anti-C5AR2 antibody

Product Synonym Names

Anti -GPR77, CT (C5a Anaphylatoxin Chemotactic Receptor C5L2, G-protein Coupled Receptor 77, C5L2)

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, 0.1% sodium azide.

Purity/Purification

Affinity Purified
Purified by immunoaffinity chromatography.

Specificity

Recognizes human GPR77.

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

36,080 Da

Chromosome Location

Chromosome: 19; NC_000019.9 (47835404..47845272). Location: 19q13.33

OMIM

609949

3D Structure

ModBase 3D Structure for Q9P296

Immunogen

Synthetic peptide corresponding to 20aa from C-terminal cytoplasmic domain of human GPR77.

Product Categories/Family for anti-C5AR2 antibody

Antibodies; Abs to G Protein Receptors

Applications Tested/Suitable for anti-C5AR2 antibody

Immunohistochemistry (IHC)

Application Notes for anti-C5AR2 antibody

Suitable for use in Immunohistochemistry.
Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml

Other Notes

Small volumes of anti-C5AR2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for C5AR2 purchase

MBS649343 is an antibody targeting C5AR2. Also known as GPR77, CT (C5a Anaphylatoxin Chemotactic Receptor C5L2, G-protein Coupled Receptor 77, C5L2). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for C5AR2. It may not necessarily be applicable to this product.

NCBI GI #

13122467

NCBI GeneID

27202

NCBI Accession #

AAK12640.1

UniProt Primary Accession #

Q9P296

UniProt Secondary Accession #

B2RA09

UniProt Related Accession #

Q9P296

NCBI Official Full Name

G protein-coupled receptor

NCBI Official Synonym Full Names

complement component 5a receptor 2

NCBI Official Symbol

C5AR2

NCBI Official Synonym Symbols

C5L2; GPF77; GPR77

UniProt Protein Name

C5a anaphylatoxin chemotactic receptor 2

UniProt Synonym Protein Names

Complement component 5a receptor 2; G-protein coupled receptor 77

Protein Family

C5a anaphylatoxin chemotactic receptor

UniProt Gene Name

C5AR2

UniProt Synonym Gene Names

C5L2; GPR77

UniProt Entry Name

C5AR2_HUMAN

NCBI Protein Information

C5a anaphylatoxin chemotactic receptor 2; G protein-coupled receptor 77; G protein-coupled receptor C5L2; C5a anaphylatoxin chemotactic receptor C5L2

NCBI Summary for C5AR2

This gene encodes a G-protein coupled receptor 1 family member involved in the complement system of the innate immune response. Unlike classical G-protein coupled receptors, the encoded protein does not associate with intracellular G-proteins. It may instead modulate signal transduction through the beta-arrestin pathway, and may alternatively act as a decoy receptor. This gene may be involved in coronary artery disease and in the pathogenesis of sepsis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]

UniProt Comments for C5AR2

Function: Receptor for the chemotactic and inflammatory C3a, C4a and C5a anaphylatoxin peptides and also for their dearginated forms ASP/C3adesArg, C4adesArg and C5adesArg respectively. Couples weakly to G(i)-mediated signaling pathways. Ref.8 Ref.10 Ref.11

Subunit structure: Interacts with C3 (the anaphylatoxin peptide C3a and the adipogenic hormone ASP); the interaction occurs with higher affinity for ASP, enhancing the phosphorylation and activation of GPR77, recruitment of ARRB2 to the cell surface and endocytosis of GRP77. Ref.9

Subcellular location: Cell membrane; Multi-pass membrane protein Ref.11.

Tissue specificity: Frontal cortex, hippocampus, hypothalamus, pons and liver. Ref.1 Ref.2

Sequence similarities: Belongs to the G-protein coupled receptor 1 family.

Research Articles on C5AR2

1. The results suggest that insulin sensitivity may be permissive for coupling of C5L2 levels to lipid storage and utilization.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-C5AR2 antibody

Products by Pathway	Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
GPCRs, Class A Rhodopsin-like Pathway antibodies	GPCRs, Class A Rhodopsin-like Pathway Diagram
GPCRs, Other Pathway antibodies	GPCRs, Other Pathway Diagram
Peptide Ligand-binding Receptors Pathway antibodies	Peptide Ligand-binding Receptors Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By GPCR Pathway antibodies	Signaling By GPCR Pathway Diagram