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HEY2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649356

MBS649356 is an antibody targeting HEY2. Also known as HEY2, NT (HEY2, BHLHB32, CHF1, GRL, HERP, HERP1, HRT2, Hairy/enhancer-of-split related with YRPW motif protein 2, Cardiovascular helix-loop-helix factor 1, Class B basic helix-loop-helix protein 32,… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
HEY2, Polyclonal Antibody
Full Product Name
HEY2, NT (HEY2, BHLHB32, CHF1, GRL, HERP, HERP1, HRT2, Hairy/enhancer-of-split related with YRPW motif protein 2, Cardiovascular helix-loop-helix factor 1, Class B basic helix-loop-helix protein 32, HES-related repressor protein 2, Hairy and enhancer of s
Product Gene Name
Product Synonym Names
Anti -HEY2, NT (HEY2, BHLHB32, CHF1, GRL, HERP, HERP1, HRT2, Hairy/enhancer-of-split related with YRPW motif protein 2, Cardiovascular helix-loop-helix factor 1, Class B basic helix-loop-helix protein 32, HES-related repressor protein 2, Hairy and enhancer of s
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human, mouse
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
35,808 Da
Chromosome Location
Chromosome: 6; NC_000006.11 (126066211..126082415). Location: 6q21
OMIM
604674
3D Structure
ModBase 3D Structure for Q9UBP5
Immunogen
HEY2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-99 amino acids from the N-terminal region of human HEY2.
Applications Tested/Suitable for anti-HEY2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-HEY2 antibody
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100

Western Blot (WB) of anti-HEY2 antibody

HEY2 Antibody (N-term) western blot analysis in mouse heart tissue lysates (15ug/lane).This demonstrates the HEY2 antibody detected HEY2 protein (arrow).
Rabbit Anti-HEY2 Polyclonal Antibody – Cat# MBS649356

Immunohistochemistry (IHC) of anti-HEY2 antibody

HEY2 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Rabbit Anti-HEY2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS649356

Other Notes

Small volumes of anti-HEY2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HEY2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
hairy/enhancer-of-split related with YRPW motif protein 2
NCBI Official Synonym Full Names
hairy/enhancer-of-split related with YRPW motif 2
NCBI Official Symbol
HEY2
NCBI Official Synonym Symbols
GRL; CHF1; HRT2; HERP1; HESR2; bHLHb32; GRIDLOCK
UniProt Protein Name
Hairy/enhancer-of-split related with YRPW motif protein 2
UniProt Synonym Protein Names
Cardiovascular helix-loop-helix factor 1; hCHF1; Class B basic helix-loop-helix protein 32; bHLHb32; HES-related repressor protein 2; Hairy and enhancer of split-related protein 2; HESR-2; Hairy-related transcription factor 2; HRT-2; hHRT2; Protein gridlock homolog
UniProt Gene Name
HEY2
UniProt Synonym Gene Names
BHLHB32; CHF1; GRL; HERP; HERP1; HRT2; hCHF1; bHLHb32; HESR-2; HRT-2; hHRT2
UniProt Entry Name
HEY2_HUMAN
NCBI Protein Information
hairy/enhancer-of-split related with YRPW motif protein 2; HRT-2; hCHF1; hHRT2; HESR-2; protein gridlock homolog; HES-related repressor protein 1; HES-related repressor protein 2; hairy-related transcription factor 2; cardiovascular helix-loop-helix factor 1; class B basic helix-loop-helix protein 32; hairy and enhancer of split-related protein 2; cardiovascular basic helix-loop-helix factor 1

NCBI Summary for HEY2

This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The encoded protein forms homo- or hetero-dimers that localize to the nucleus and interact with a histone deacetylase complex to repress transcription. Expression of this gene is induced by the Notch signal transduction pathway. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternatively spliced transcript variants have been found, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
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UniProt Comments for HEY2

Function: Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6. Ref.3 Ref.4 Ref.10 Ref.11

Subunit structure: May self-associate

By similarity. Interacts with GATA4, HES1 and HEYL

By similarity. Interacts with HDAC1, NCOR1 and SIN3A

By similarity. Interacts with ARNT and GATA6. Ref.3 Ref.11

Subcellular location: Nucleus

By similarity.

Sequence similarities: Belongs to the HEY family.Contains 1 bHLH (basic helix-loop-helix) domain.Contains 1 Orange domain.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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