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Themis Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649401

Themis also called Gasp (Grb2-associating protein), is a 72kD, 641aa cytosolic phosphoprotein mainly expressed in late double-negative and double-positive thymocytes. It is involved in thymocyte positive and negative selection through regulation of TCR… More details

$980 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Themis, Monoclonal Antibody
Full Product Name
Themis (Protein THEMIS, Grb2-associating Protein, Gasp, Protein Thylex, Thymocyte-expressed Molecule Involved in Selection)
Product Synonym Names
Anti -Themis (Protein THEMIS, Grb2-associating Protein, Gasp, Protein Thylex, Thymocyte-expressed Molecule Involved in Selection)
Clonality
Monoclonal
Isotype
IgG2B
Clone Number
12K186
Host
Rat
Species Reactivity
Mouse
Form/Format
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
Purity/Purification
Affinity Purified
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes mouse Themis. Species sequence homology: Recombinant human Themis aa2-282 (100%).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant corresponding to aa122-237 from mouse Themis expressed in E.coli.

Product Categories/Family for anti-Themis antibody

Applications Tested/Suitable for anti-Themis antibody
Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-Themis antibody
Suitable for use in Flow Cytometry and Western Blot.
Dilution: Flow Cytometry: 2.5ug labels 10e6
Western Blot: 1ug/ml

Western Blot (WB) of anti-Themis antibody

Detection of Mouse Themis by Western Blot. Western blot shows lysates of mouse thymus tissue. PVDF membrane was probed with 1ug/ml of Rat Mouse Themis Monoclonal Antibody followed by HRP-conjugated Rat IgG Secondary Antibody. A specific band was detected for Themis at approximately 72kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Rat Anti-Themis Monoclonal Antibody – Cat# MBS649401

Flow Cytometry (FC/FACS) of anti-Themis antibody

Detection of Themis in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Mouse Themis Monoclonal Antibody or isotype control antibody, followed by Phy-coerythrin -conjugated Rat IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Rat Anti-Themis Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS649401

Other Notes

Small volumes of anti-Themis antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Themis purchase

MBS649401 is an antibody targeting Themis. Also known as Themis (Protein THEMIS, Grb2-associating Protein, Gasp, Protein Thylex, Thymocyte-expressed Molecule Involved in Selection). Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for Themis. It may not necessarily be applicable to this product.

Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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