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PIM2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649417

Pim proteins (Pim-1, Pim-2 and Pim-3) are oncogene-encoded serine/threonine kinases. Pim-2 is highly homologous to Pim-1 with similar oncogenic functions. Pim-2 overexpression promotes resistance to a host of apoptotic stimuli; its expression is negatively… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PIM2, Polyclonal Antibody
Full Product Name
PIM2, CT (PIM2, Serine/threonine-protein kinase pim-2, Pim-2h)
Product Synonym Names
Anti -PIM2, CT (PIM2, Serine/threonine-protein kinase pim-2, Pim-2h)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
PIM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 282~311 amino acids from the C-terminal region of human PIM2.
Applications Tested/Suitable for anti-PIM2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-PIM2 antibody
Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Immunoprecipitation

Western Blot (WB) of anti-PIM2 antibody

Western Blot analysis of MBS649417 in Hela cell line lysates (35ug/lane). PIM2 (arrow) was detected using the purified Pab.
Rabbit Anti-PIM2 Polyclonal Antibody – Cat# MBS649417

Western Blot (WB) of anti-PIM2 antibody

Western Blot analysis of PIM2 (arrow) using MBS649417. 293 cell lysates (2ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIM2 gene.
Rabbit Anti-PIM2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS649417

Western Blot (WB) of anti-PIM2 antibody

PIM proteins were immunoprecipitated from MV4;11 cells and the agarose-protein A-immunoprecipitate complex was tested for its ability to phosphorylate BAD in vitro in the presence or absence of K00135. Phosphorylation of BAD (both on Ser112 and Ser136, detected by WB with phospho-specific antibodies) was abrogated on addition of the compound. Asterisks, strong bands corresponding to the heavy chain of the MBS649417 recognized by the antirabbit immunoglobulin G secondary antibody. Beads alone (without anti-PIM antibodies) were incubated with the MV4;11 extract and used for the same in vitro phosphorylation reaction as a negative control.
Rabbit Anti-PIM2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS649417

Immunohistochemistry (IHC) of anti-PIM2 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with MBS649417, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Rabbit Anti-PIM2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS649417

Other Notes

Small volumes of anti-PIM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PIM2 purchase

MBS649417 is an antibody targeting PIM2. Also known as PIM2, CT (PIM2, Serine/threonine-protein kinase pim-2, Pim-2h). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PIM2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
Pim2

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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