RNF14 Antibody – Rabbit Polyclonal

The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Six alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq, Jan 2011]

Function: Might act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which could be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription. Ref.11

Pathway: Protein modification; protein ubiquitination.

Subunit structure: Interacts with the ubiquitin-conjugating enzymes UBE2E1 and UBE2E2. Interacts with AR/androgen receptor; testosterone- and RNF6-regulated it promotes AR transcriptional activity. Ref.11

Subcellular location: Cytoplasm. Nucleus Ref.8.

Tissue specificity: Widely expressed.

Domain: The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway

By similarity.The RING-type zinc finger is essential for the interaction with UBE2E2.

Post-translational modification: RING-type zinc finger-dependent and UBE2E2-dependent autoubiquitination.

Sequence similarities: Belongs to the RBR family. RNF14 subfamily.Contains 1 IBR-type zinc finger.Contains 2 RING-type zinc fingers.Contains 1 RWD domain.

Caution: Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family.