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DCAMKL1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649427

Doublecortin-like kinase (DCAMKL1)(Ser/Thr protein kinase family) is essential for proper neurogenesis, neuronal migration, and axonal wiring. DCAMKL1 is involved in a calcium-signaling pathway controling neuronal migration in the developing brain, and… More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
DCAMKL1, Polyclonal Antibody
Full Product Name
DCAMKL1, CT (DCLK1, DCAMKL1, DCDC3A, KIAA0369, Serine/threonine-protein kinase DCLK1, Doublecortin domain-containing protein 3A, Doublecortin-like and CAM kinase-like 1, Doublecortin-like kinase 1)
Product Synonym Names
Anti -DCAMKL1, CT (DCLK1, DCAMKL1, DCDC3A, KIAA0369, Serine/threonine-protein kinase DCLK1, Doublecortin domain-containing protein 3A, Doublecortin-like and CAM kinase-like 1, Doublecortin-like kinase 1)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Human, mouse
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
DCAMKL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 689~718 amino acids from the C-terminal region of human DCAMKL1.

Product Categories/Family for anti-DCAMKL1 antibody

Antibodies; Abs to Protein Kinases
Applications Tested/Suitable for anti-DCAMKL1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-DCAMKL1 antibody
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
immunofluorescence

Western Blot (WB) of anti-DCAMKL1 antibody

Western Blot analysis in mouse heart tissue lysates (35ug/lane) using MBS649427. This demonstrates that MBS649427 detected the DCAMKL1 protein (arrow).
Rabbit Anti-DCAMKL1 Polyclonal Antibody – Cat# MBS649427

Western Blot (WB) of anti-DCAMKL1 antibody

Western Blot analysis in ZR-75-1 cell line lysates (35ug/lane) using MBS649427. This demonstrates that MBS649427 detected the DCAMKL1 protein (arrow).
Rabbit Anti-DCAMKL1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS649427

Immunohistochemistry (IHC) of anti-DCAMKL1 antibody

Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MBS649427, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS649427 for immunohistochemistry.
Rabbit Anti-DCAMKL1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS649427

Immunohistochemistry (IHC) of anti-DCAMKL1 antibody

Expression pattern of doublecortin and CaM kinase-like-1 (DCAMKL-1) in normal mouse small intestine. (A): Immunohistochemical staining of normal small intestine for DCAMKL-1. Arrows indicate the cells positive for DCAMKL-1 in the stem cell zone. (B): Preincubation with blocking peptide completely abolished DCAMKL-1 immunoreactivity. (C): Immunohistochemical staining of normal small intestine for DCAMKL-1. Brown indicates the cells positive for DCAMKL-1 (indicated by the arrows).
Rabbit Anti-DCAMKL1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS649427

Testing Data of anti-DCAMKL1 antibody

(C): The cell positive for DCAMKL-1, stained red (indicated by the arrow), appears at the base of the crypt. (D): Intestinal section stained for Musashi-1 (green). (E): Colocalization of DCAMKL-1 and Musashi-1 (yellow, indicated by the arrow). The magnified inset image represents the single cell positive for both DCAMKL-1 and Musashi-1. (F): Costaining of DCMAKL-1 (red, indicated by the arrow) with nuclear Hoechst 33342 (blue) staining. (G): Costaining of Musashi-1 (green) with nuclear Hoechst 33342 (blue) staining. (H): Colocalization of DCAMKL-1 and Musashi-1 (yellow, indicated by the arrow), costained with nuclear Hoechst 33342 (blue) staining. The magnified inset image represents the single cell positive for both DCAMKL-1 and Musashi-1 (yellow).
Rabbit Anti-DCAMKL1 Polyclonal Antibody, Testing Data – Cat# MBS649427

Other Notes

Small volumes of anti-DCAMKL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DCAMKL1 purchase

MBS649427 is an antibody targeting DCAMKL1. Also known as DCAMKL1, CT (DCLK1, DCAMKL1, DCDC3A, KIAA0369, Serine/threonine-protein kinase DCLK1, Doublecortin domain-containing protein 3A, Doublecortin-like and CAM kinase-like 1, Doublecortin-like kinase 1). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DCAMKL1. It may not necessarily be applicable to this product.

NCBI GI #
117616780
NCBI Accession #
ABK42408.1
NCBI Official Full Name
Dcamkl1

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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