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POU3F3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649445

POU3F3 is a member of the class III POU family of transcription factors (see POU3F1; MIM 602479) that are expressed in the central nervous system. The POU domain in these proteins is required for high affinity binding to octamer DNA sequences (summarized by… More details

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Product Overview

Online Datasheet in PDF
Product Name
POU3F3, Polyclonal Antibody
Full Product Name
POU3F3, ID (POU3F3, BRN1, OTF8, POU domain, class 3, transcription factor 3, Brain-specific homeobox/POU domain protein 1, Octamer-binding protein 8, Octamer-binding transcription factor 8)
Product Gene Name
Product Synonym Names
Anti -POU3F3, ID (POU3F3, BRN1, OTF8, POU domain, class 3, transcription factor 3, Brain-specific homeobox/POU domain protein 1, Octamer-binding protein 8, Octamer-binding transcription factor 8)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
50,327 Da
Chromosome Location
Chromosome: 2; NC_000002.11 (105471969..105473471). Location: 2q12.1
OMIM
602480
3D Structure
ModBase 3D Structure for P20264
Immunogen
POU3F3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 293-323 amino acids from the Central region of human POU3F3.
Applications Tested/Suitable for anti-POU3F3 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-POU3F3 antibody
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500

Western Blot (WB) of anti-POU3F3 antibody

POU3F3 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the POU3F3 antibody detected the POU3F3 protein (arrow).
Rabbit Anti-POU3F3 Polyclonal Antibody – Cat# MBS649445

Other Notes

Small volumes of anti-POU3F3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for POU3F3 purchase

MBS649445 is an antibody targeting POU3F3. Also known as POU3F3, ID (POU3F3, BRN1, OTF8, POU domain, class 3, transcription factor 3, Brain-specific homeobox/POU domain protein 1, Octamer-binding protein 8, Octamer-binding transcription factor 8). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for POU3F3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
POU domain, class 3, transcription factor 3
NCBI Official Synonym Full Names
POU class 3 homeobox 3
NCBI Official Symbol
POU3F3
NCBI Official Synonym Symbols
BRN1; OTF8
UniProt Protein Name
POU domain, class 3, transcription factor 3
UniProt Synonym Protein Names
Brain-specific homeobox/POU domain protein 1; Brain-1; Brn-1; Octamer-binding protein 8; Oct-8; Octamer-binding transcription factor 8
UniProt Gene Name
POU3F3
UniProt Synonym Gene Names
BRN1; OTF8; Brain-1; Brn-1; Oct-8; OTF-8
UniProt Entry Name
PO3F3_HUMAN
NCBI Protein Information
POU domain, class 3, transcription factor 3; OTF-8; brn-1; oct-8; brain-1; octamer-binding protein 8; octamer-binding transcription factor 8; brain-specific homeobox/POU domain protein 1

NCBI Summary for POU3F3

POU3F3 is a member of the class III POU family of transcription factors (see POU3F1; MIM 602479) that are expressed in the central nervous system. The POU domain in these proteins is required for high affinity binding to octamer DNA sequences (summarized by Sumiyama et al., 1996 [PubMed 8703082]).[supplied by OMIM, Jul 2010]
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UniProt Comments for POU3F3

Function: Transcription factor that acts synergistically with SOX11 and SOX4. Plays a role in neuronal development. Is implicated in an enhancer activity at the embryonic met-mesencephalic junction; the enhancer element contains the octamer motif (5'-ATTTGCAT-3')

By similarity.

Subcellular location: Nucleus.

Tissue specificity: Brain.

Sequence similarities: Belongs to the POU transcription factor family. Class-3 subfamily.Contains 1 homeobox DNA-binding domain.Contains 1 POU-specific domain.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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