Rabbit Anti-TONSL Polyclonal Antibody – Cat# MBS649449

NFKBIL2 Antibody (N-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the NFKBIL2 antibody detected the NFKBIL2 protein (arrow).

NFKBIL2 (TONSL) Antibody – Rabbit Polyclonal

Name: TONSL Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS649449
Price: 895 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649449

The protein encoded by this gene is thought to be a negative regulator of NF-kappa-B mediated transcription. The encoded protein may bind NF-kappa-B complexes and trap them in the cytoplasm, preventing them from entering the nucleus and interacting with the… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NFKBIL2 (TONSL), Polyclonal Antibody

Full Product Name

NFKBIL2, NT (TONSL, IKBR, NFKBIL2, Tonsoku-like protein, Inhibitor of kappa B-related protein, NF-kappa-B inhibitor-like protein 2, Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 2)

Product Gene Name

anti-TONSL antibody

Product Synonym Names

Anti -NFKBIL2, NT (TONSL, IKBR, NFKBIL2, Tonsoku-like protein, Inhibitor of kappa B-related protein, NF-kappa-B inhibitor-like protein 2, Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 2)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Human

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

150,929 Da

Chromosome Location

Chromosome: 8; NC_000008.10 (145654163..145669871, complement). Location: 8q24.3

OMIM

604546

3D Structure

ModBase 3D Structure for Q96HA7

Immunogen

NFKBIL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-65 amino acids from the N-terminal region of human NFKBIL2.

Product Categories/Family for anti-TONSL antibody

Antibodies; Abs to Protein Kinase Inhibitors

Applications Tested/Suitable for anti-TONSL antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-TONSL antibody

Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500

Other Notes

Small volumes of anti-TONSL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TONSL purchase

MBS649449 is an antibody targeting TONSL. Also known as NFKBIL2, NT (TONSL, IKBR, NFKBIL2, Tonsoku-like protein, Inhibitor of kappa B-related protein, NF-kappa-B inhibitor-like protein 2, Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 2). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TONSL. It may not necessarily be applicable to this product.

NCBI GI #

119602499

NCBI GeneID

4796

NCBI Accession #

EAW82093.1

UniProt Primary Accession #

Q96HA7

UniProt Secondary Accession #

Q13006; Q9UGJ2; B5MDP0; C9JKB1; C9JNV8

UniProt Related Accession #

Q96HA7

NCBI Official Full Name

nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 2, isoform CRA_b

NCBI Official Synonym Full Names

tonsoku-like, DNA repair protein

NCBI Official Symbol

TONSL

NCBI Official Synonym Symbols

IKBR; NFKBIL2

UniProt Protein Name

Tonsoku-like protein

UniProt Synonym Protein Names

Inhibitor of kappa B-related protein; I-kappa-B-related protein; IkappaBR; NF-kappa-B inhibitor-like protein 2; Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 2

UniProt Gene Name

TONSL

UniProt Synonym Gene Names

IKBR; NFKBIL2; I-kappa-B-related protein; IkappaBR

UniProt Entry Name

TONSL_HUMAN

NCBI Protein Information

tonsoku-like protein; ikappaBR; I-kappa-B-related protein; NF-kappa-B inhibitor-like protein 2; inhibitor of kappa B-related protein; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 2

UniProt Comments for TONSL

Function: Component of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork-associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA. Within the complex, may act as a scaffold. Ref.1 Ref.7 Ref.8 Ref.9

Subunit structure: Component of the MMS22L-TONSL complex, a complex at least composed of MMS22L and TONSL/NFKBIL2. Interacts with the MCM complex, the FACT complex and the RPA complex. Binds histones. Ref.7 Ref.8 Ref.9

Subcellular location: Cytoplasm. Nucleus. Note: Mainly nuclear. Localizes to DNA damage sites, accumulates at stressed replication forks. Ref.5 Ref.7 Ref.8

Tissue specificity: Expressed in heart, skeletal muscle and tracheal epithelial cells. Ref.1

Domain: The ANK repeats mediate the interaction with the MCM complex and histones, while the LRR repeats mediate the interaction with MMS22L. Ref.8

Sequence similarities: Belongs to the Tonsoku family.Contains 3 ANK repeats.Contains 7 LRR (leucine-rich) repeats.Contains 8 TPR repeats.

Caution: Was reported to share sequence similarities with IKBKB and therefore named 'NF-kappa-B inhibitor-like protein 2' (Ref.1). However, the sequence similarity is remote and effects as regulator of NF-kappa-B are probably indirect and require additional evidence (Ref.5).

Sequence caution: The sequence AAA85819.1 differs from that shown. Reason: Frameshift at several positions. The sequence AAH08782.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence CAB63467.1 differs from that shown. Reason: Erroneous gene model prediction.

Research Articles on TONSL

1. Common NFKBIL2 polymorphisms are associated with susceptibility to invasive pneumococcal infection.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TONSL antibody

Reference Product	PubMed Publications
C6orf167 antibody	>3 publications with TONSL and C6orf167
ASF1B antibody	>1 publications with TONSL and ASF1B
ASF1A antibody	>1 publications with TONSL and ASF1A

Diseases associated with anti-TONSL antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>3 publications with TONSL and Inflammation
Nervous System Diseases Antibodies	>1 publications with TONSL and Nervous System Diseases
Drug Hypersensitivity Antibodies	>1 publications with TONSL and Drug Hypersensitivity
Necrosis Antibodies	>1 publications with TONSL and Necrosis

Organs/Tissues associated with anti-TONSL antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>3 publications with TONSL and Lung
Blood Antibodies	>2 publications with TONSL and Blood
Brain Antibodies	>2 publications with TONSL and Brain
Placenta Antibodies	>1 publications with TONSL and Placenta
Kidney Antibodies	>1 publications with TONSL and Kidney
Intestine Antibodies	>1 publications with TONSL and Intestine