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PI3KCA Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649465

Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has… More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PI3KCA, Polyclonal Antibody
Full Product Name
PI3KCA, ID (PIK3CA, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110kD catalytic subunit alpha, Phosphoinositide-3-kinase catalytic alpha polypeptide, Serine/threonine prote
Product Synonym Names
Anti -PI3KCA, ID (PIK3CA, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110kD catalytic subunit alpha, Phosphoinositide-3-kinase catalytic alpha polypeptide, Serine/threonine prote
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
PI3KCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 511~541 amino acids from the the center region of human PI3KCA.

Product Categories/Family for anti-PI3KCA antibody

Antibodies; Abs to Protein Kinases
Applications Tested/Suitable for anti-PI3KCA antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-PI3KCA antibody
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:50-100
Immunohistochemistry: 1:50-100

Western Blot (WB) of anti-PI3KCA antibody

Western blot analysis of anti-PIK3CA Antibody (Center) Pab in K562 cell line lysates (35ug/lane). PIK3CA (arrow) was detected using the purified Pab
Rabbit Anti-PI3KCA Polyclonal Antibody – Cat# MBS649465

Western Blot (WB) of anti-PI3KCA antibody

Western blot analysis of anti-PI3KCA Antibody (Center) Pab in 293 cell line lysates transiently transfected with the PIK3CA gene (1ug/lane). PI3KCA (arrow) was detected using the purified Pab.
Rabbit Anti-PI3KCA Polyclonal Antibody, Western Blot (WB) – Cat# MBS649465

Immunohistochemistry (IHC) of anti-PI3KCA antibody

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PI3KCA Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-PI3KCA Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS649465

Other Notes

Small volumes of anti-PI3KCA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PI3KCA purchase

MBS649465 is an antibody targeting PI3KCA. Also known as PI3KCA, ID (PIK3CA, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110kD catalytic subunit alpha, Phosphoinositide-3-kinase catalytic alpha polypeptide, Serine/threonine prote. Reactive in Human. Host: Rabbit.
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