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BCL9 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649509

BCL9 is involved in signal transduction through the Wnt pathway. It promotes beta-catenin's transcriptional activity. BCL9 is associated with B-cell acute lymphoblastic leukemia. The overexpression of BCL9 may be of pathogenic significance in B-cell… More details

$880 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BCL9, Monoclonal Antibody
Full Product Name
BCL9 (B-cell CLL/Lymphoma 9 Protein, B-cell Lymphoma 9 Protein, Bcl-9, Protein Legless Homolog)
Product Gene Name
anti-BCL9 antibody
Product Synonym Names
Anti -BCL9 (B-cell CLL/Lymphoma 9 Protein, B-cell Lymphoma 9 Protein, Bcl-9, Protein Legless Homolog)
Immunogen Sequence
DDDSPPARSP NLPSMNNMPG MGINTQNPRI SGPNPVVPMP TLSPMGMTQP LSHSNQMPSP NAVGPNIPPH GVPMGPGLMS HNPIMGHGSQ EPPMVPQGRM
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
2D4
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human BCL9.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
149,290 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (147013222..147098022). Location: 1q21
OMIM
602597
3D Structure
ModBase 3D Structure for O00512
Immunogen
Partial recombinant corresponding to aa1036-1135 from human BCL9 (NP_004317) with GST tag. MW of the GST tag alone is 26kD.

Product Categories/Family for anti-BCL9 antibody

Antibodies; Abs to Apoptosis
Applications Tested/Suitable for anti-BCL9 antibody
ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-BCL9 antibody
Suitable for use in Immunofluorescence, ELISA and Western Blot.
Dilution: Immunofluorescence: 10ug/ml

Western Blot (WB) of anti-BCL9 antibody

Western Blot detection against Immunogen (36.74kD).
Mouse Anti-BCL9 Monoclonal Antibody – Cat# MBS649509

Immunofluorescence (IF) of anti-BCL9 antibody

Immunofluorescence of monoclonal antibody to BCL9 on HeLa cell. [antibody concentration 10ug/ml].
Mouse Anti-BCL9 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS649509

Testing Data of anti-BCL9 antibody

Detection limit for recombinant GST tagged BCL9 is ~0.03ng/ml as a capture antibody.
Mouse Anti-BCL9 Monoclonal Antibody, Testing Data – Cat# MBS649509

Other Notes

Small volumes of anti-BCL9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BCL9 purchase

MBS649509 is an antibody targeting BCL9. Also known as BCL9 (B-cell CLL/Lymphoma 9 Protein, B-cell Lymphoma 9 Protein, Bcl-9, Protein Legless Homolog). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for BCL9. It may not necessarily be applicable to this product.

NCBI GI #
72256200
NCBI GeneID
607
NCBI Accession #
NP_004317.2
UniProt Primary Accession #
O00512
UniProt Secondary Accession #
Q5T489
UniProt Related Accession #
O00512
GenBank Nucleotide #
NM_004326.3
NCBI Official Full Name
B-cell CLL/lymphoma 9 protein
NCBI Official Synonym Full Names
B-cell CLL/lymphoma 9
NCBI Official Symbol
BCL9
NCBI Official Synonym Symbols
LGS
UniProt Protein Name
B-cell CLL/lymphoma 9 protein
UniProt Synonym Protein Names
Protein legless homolog
Protein Family
B-cell CLL/lymphoma 9 protein
UniProt Gene Name
BCL9
UniProt Synonym Gene Names
B-cell lymphoma 9 protein; Bcl-9
UniProt Entry Name
BCL9_HUMAN
NCBI Protein Information
B-cell CLL/lymphoma 9 protein; bcl-9; protein legless homolog; B-cell lymphoma 9 protein

NCBI Summary for BCL9

BCL9 is associated with B-cell acute lymphoblastic leukemia. It may be a target of translocation in B-cell malignancies with abnormalities of 1q21. Its function is unknown. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies. [provided by RefSeq, Jul 2008]

UniProt Comments for BCL9

Function: Involved in signal transduction through the Wnt pathway. Promotes beta-catenin's transcriptional activity

By similarity. Ref.4

Subunit structure: Binds to beta-catenin (CTNNB1), PYGO1 and PYGO2; the interaction with PYGO1 increases PYGO1 affinity to histone H3 methylated at 'Lys 4'. Ref.11

Subcellular location: Nucleus

By similarity.

Tissue specificity: Detected at low levels in thymus, prostate, testis, ovary and small intestine, and at lower levels in spleen, colon and blood.

Involvement in disease: A chromosomal aberration involving BCL9 is found in a patient with precusor B-cell acute lymphoblastic leukemia (ALL). Translocation t(1;14)(q21;q32). This translocation leaves the coding region intact, but may have pathogenic effects due to alterations in the expression level of BCL9. Several cases of translocations within the 3'-UTR of BCL9 have been found in B-cell malignancies.

Sequence similarities: Belongs to the BCL9 family.

Caution: It is uncertain whether Met-1 or Met-27 is the initiator.

Sequence caution: The sequence CAA73942.1 differs from that shown. Reason: Frameshift at position 1391.
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Research Articles on BCL9

1. we detected five SNPs in the first two genes/loci - BCL9 and C9orf5 - strongly associated with negative symptoms of schizophrenia

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BCL9 antibody

 Reference Product  PubMed Publications
 CTNNB1 antibody  >41 publications with BCL9 and CTNNB1
 PYGO1 antibody  >8 publications with BCL9 and PYGO1
 PYGO2 antibody  >6 publications with BCL9 and PYGO2
 TCF4 antibody  >4 publications with BCL9 and TCF4
 TCF7L2 antibody  >3 publications with BCL9 and TCF7L2

Pathways associated with anti-BCL9 antibody

 Products by Pathway  Pathway Diagram
 Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway antibodies  Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway Diagram
 Wnt Signaling Pathway NetPath antibodies  Wnt Signaling Pathway NetPath Diagram

Diseases associated with anti-BCL9 antibody

 Disease Name  Pubmed Publications
 Neoplasm Metastasis Antibodies  >4 publications with BCL9 and Neoplasm Metastasis
 Abnormalities, Multiple Antibodies  >2 publications with BCL9 and Abnormalities, Multiple
 Liver Diseases Antibodies  >1 publications with BCL9 and Liver Diseases
 Depressive Disorder Antibodies  >1 publications with BCL9 and Depressive Disorder
 Ovarian Diseases Antibodies  >1 publications with BCL9 and Ovarian Diseases
 Necrosis Antibodies  >1 publications with BCL9 and Necrosis
 Ovarian Neoplasms Antibodies  >1 publications with BCL9 and Ovarian Neoplasms

Organs/Tissues associated with anti-BCL9 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >4 publications with BCL9 and Bone
 Liver Antibodies  >4 publications with BCL9 and Liver
 Intestine Antibodies  >3 publications with BCL9 and Intestine
 Embryonic Tissue Antibodies  >3 publications with BCL9 and Embryonic Tissue
 Brain Antibodies  >3 publications with BCL9 and Brain
 Placenta Antibodies  >2 publications with BCL9 and Placenta
 Lung Antibodies  >2 publications with BCL9 and Lung
 Kidney Antibodies  >1 publications with BCL9 and Kidney
 Prostate Antibodies  >1 publications with BCL9 and Prostate
 Eye Antibodies  >1 publications with BCL9 and Eye
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