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MAP3K11 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649565

MLK3 (Mixed lineage kinase 3) is a serine/threonine kinase that has an amino-terminal SH3 domain followed by the kinase domain and two leucine zippers, a cdc42/Rac1 binding (CRIB) domain and several other domains/motifs at the carboxy-terminal region. MLK3… More details

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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MLK3 (MAP3K11), Monoclonal Antibody
Full Product Name
MLK3 (Mixed Lineage Kinase 3, MLK-3, Mitogen-activated Protein Kinase Kinase Kinase 11, MAP3K11, MEKK11, PTK1, Src-homology 3 Domain-containing Proline-rich Kinase, SPRK, MGC17114)
Product Gene Name
anti-MAP3K11 antibody
Product Synonym Names
Anti -MLK3 (Mixed Lineage Kinase 3, MLK-3, Mitogen-activated Protein Kinase Kinase Kinase 11, MAP3K11, MEKK11, PTK1, Src-homology 3 Domain-containing Proline-rich Kinase, SPRK, MGC17114)
Immunogen Sequence
PPPGTSRSAP GTPGTPRSPP LGLISRPRPS PLRSRIDPWS FVSAGPRPSP LPSPQPAPRR APWTLFPDSD PFWDSPPANP FQGGPQDCRA QTKDMGAQAP WVPEAGP
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
3D11
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human MAP3K11.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
92,688 Da
Chromosome Location
Chromosome: 11; NC_000011.9 (65365226..65381720, complement). Location: 11q13.1-q13.3
OMIM
600050
3D Structure
ModBase 3D Structure for Q16584
Immunogen
Partial recombinant corresponding to aa741-847 from human MAP3K11 (NP_002410) with GST tag. MW of the GST tag alone is 26kD.

Product Categories/Family for anti-MAP3K11 antibody

Antibodies; Abs to Protein Kinases
Applications Tested/Suitable for anti-MAP3K11 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-MAP3K11 antibody
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml

Western Blot (WB) of anti-MAP3K11 antibody

Western Blot detection against Immunogen (37.51kD).
Mouse Anti-MAP3K11 Monoclonal Antibody – Cat# MBS649565

Western Blot (WB) of anti-MAP3K11 antibody

Western Blot analysis of MAP3K11 expression in transfected 293T cell line by MAP3K11 monoclonal antibody.|Lane 1: MAP3K11 transfected lysate (92.7kD).|Lane 2: Non-transfected lysate.
Mouse Anti-MAP3K11 Monoclonal Antibody, Western Blot (WB) – Cat# MBS649565

Immunohistochemistry (IHC) of anti-MAP3K11 antibody

Immunoperoxidase of monoclonal antibody to MAP3K11 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3ug/ml]
Mouse Anti-MAP3K11 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS649565

Testing Data of anti-MAP3K11 antibody

Detection limit for recombinant GST tagged MAP3K11 is 0.1ng/ml as a capture antibody.
Mouse Anti-MAP3K11 Monoclonal Antibody, Testing Data – Cat# MBS649565

Testing Data of anti-MAP3K11 antibody

Proximity Ligation Analysis of protein-protein interactions between CDC42 and MAP3K11 HeLa cells were stained with anti-CDC42 rabbit purified polyclonal 1:1200 and anti-MAP3K11 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Mouse Anti-MAP3K11 Monoclonal Antibody, Testing Data – Cat# MBS649565

Other Notes

Small volumes of anti-MAP3K11 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MAP3K11 purchase

MBS649565 is an antibody targeting MAP3K11. Also known as MLK3 (Mixed Lineage Kinase 3, MLK-3, Mitogen-activated Protein Kinase Kinase Kinase 11, MAP3K11, MEKK11, PTK1, Src-homology 3 Domain-containing Proline-rich Kinase, SPRK, MGC17114). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MAP3K11. It may not necessarily be applicable to this product.

NCBI GI #
4505195
NCBI GeneID
4296
NCBI Accession #
NP_002410.1
UniProt Primary Accession #
Q16584
UniProt Secondary Accession #
Q53H00; Q59F06; Q6P2G4
UniProt Related Accession #
Q16584
GenBank Nucleotide #
NM_002419.3
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase 11
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase 11
NCBI Official Symbol
MAP3K11
NCBI Official Synonym Symbols
MLK3; PTK1; SPRK; MLK-3; MEKK11
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 11
UniProt Synonym Protein Names
Mixed lineage kinase 3; Src-homology 3 domain-containing proline-rich kinase
Protein Family
Mitogen-activated protein kinase kinase kinase
UniProt Gene Name
MAP3K11
UniProt Synonym Gene Names
MLK3; PTK1; SPRK
UniProt Entry Name
M3K11_HUMAN
NCBI Protein Information
mitogen-activated protein kinase kinase kinase 11; mixed lineage kinase 3; protein-tyrosine kinase PTK1; SH3 domain-containing proline-rich kinase; src-homology 3 domain-containing proline-rich kinase

NCBI Summary for MAP3K11

The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. [provided by RefSeq, Jul 2008]
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UniProt Comments for MAP3K11

Function: Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle. Ref.1 Ref.5 Ref.9 Ref.10

Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Ref.1

Cofactor: Magnesium

By similarity.

Enzyme regulation: Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation. Ref.6 Ref.7

Subunit structure: Homodimer; undergoes dimerization during activation. Interacts with MAP2K4/MKK4. Interacts with MAP2K7/MKK7

By similarity. Ref.5 Ref.6

Subcellular location: Cytoplasm › cytoskeleton › microtubule organizing center › centrosome. Note: Location is cell cycle dependent. Ref.9

Tissue specificity: Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain. Ref.1 Ref.2

Post-translational modification: Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42.

Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.Contains 1 protein kinase domain.Contains 1 SH3 domain.

Sequence caution: The sequence BAD92892.1 differs from that shown. Reason: Erroneous initiation.
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Research Articles on MAP3K11

1. Lysine 63-linked ubiquitination modulates mixed lineage kinase-3 interaction with JIP1 scaffold protein in cytokine-induced pancreatic beta cell death

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MAP3K11 antibody

 Reference Product  PubMed Publications
 MAP3K1 antibody  >1 publications with MAP3K11 and MAP3K1

Pathways associated with anti-MAP3K11 antibody

 Products by Pathway  Pathway Diagram
 CDC42 Signaling Events Pathway antibodies  CDC42 Signaling Events Pathway Diagram
 IFN-gamma Pathway antibodies  IFN-gamma Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Non-alcoholic Fatty Liver Disease (NAFLD) Pathway antibodies  Non-alcoholic Fatty Liver Disease (NAFLD) Pathway Diagram
 Non-alcoholic Fatty Liver Disease (NAFLD) Pathway antibodies  Non-alcoholic Fatty Liver Disease (NAFLD) Pathway Diagram
 RAC1 Signaling Pathway antibodies  RAC1 Signaling Pathway Diagram
 Reelin Signaling Pathway antibodies  Reelin Signaling Pathway Diagram

Diseases associated with anti-MAP3K11 antibody

 Disease Name  Pubmed Publications
 Hearing Loss Antibodies  >1 publications with MAP3K11 and Hearing Loss
 Nervous System Diseases Antibodies  >1 publications with MAP3K11 and Nervous System Diseases
 Immune System Diseases Antibodies  >1 publications with MAP3K11 and Immune System Diseases
 Breast Neoplasms Antibodies  >1 publications with MAP3K11 and Breast Neoplasms
 Inflammation Antibodies  >1 publications with MAP3K11 and Inflammation

Organs/Tissues associated with anti-MAP3K11 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >3 publications with MAP3K11 and Testis
 Blood Antibodies  >1 publications with MAP3K11 and Blood
 Adrenal Gland Antibodies  >1 publications with MAP3K11 and Adrenal Gland
 Uterus Antibodies  >1 publications with MAP3K11 and Uterus
 Prostate Antibodies  >1 publications with MAP3K11 and Prostate
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