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DRAM1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649575

Damage-regulated autophagy modulator (DRAM) is a p53 target gene encoding a lysosomal protein that induces autophagy, a process that degrades cytosolic proteins and organelles. It has been suggested that activation of DRAM by p53 is simultaneous to the… More details

$915 / 0.05 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
DRAM1, Polyclonal Antibody
Full Product Name
DRAM1, NT (DRAM, DNA Damage-regulated Autophagy Modulator Protein 1, Damage-regulated Autophagy Modulator)
Product Gene Name
Product Synonym Names
Anti -DRAM1, NT (DRAM, DNA Damage-regulated Autophagy Modulator Protein 1, Damage-regulated Autophagy Modulator)
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes human DRAM. Species sequence homology: mouse and rat
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
26,253 Da
Chromosome Location
Chromosome: 12; NC_000012.11 (102271105..102317401). Location: 12q23.2
OMIM
610776
3D Structure
ModBase 3D Structure for Q8N682
Immunogen
Synthetic peptide corresponding to 16aa from near the N-terminal of human DRAM (KLH).

Product Categories/Family for anti-DRAM1 antibody

Applications Tested/Suitable for anti-DRAM1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-DRAM1 antibody
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: Western Blot: 0.5-1ug/ml
Immunohistochemistry: 2.5ug/ml
Immunohistochemistry (Formalin-fixed, paraffin-embedded): 3ug/ml

Immunohistochemistry (IHC) of anti-DRAM1 antibody

Human Prostate (formalin-fixed, paraffin-embedded) stained with DRAM antibody at 3ug/ml followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.
Rabbit Anti-DRAM1 Polyclonal Antibody – Cat# MBS649575

Western Blot (WB) of anti-DRAM1 antibody

Western blot analysis of DRAM in K562 cell lysate with DRAM antibody at (A) 0.5, (B) 1 and (C) 2ug/ml.
Rabbit Anti-DRAM1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS649575

Other Notes

Small volumes of anti-DRAM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DRAM1 purchase

MBS649575 is an antibody targeting DRAM1. Also known as DRAM1, NT (DRAM, DNA Damage-regulated Autophagy Modulator Protein 1, Damage-regulated Autophagy Modulator). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DRAM1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA damage-regulated autophagy modulator protein 1
NCBI Official Synonym Full Names
DNA-damage regulated autophagy modulator 1
NCBI Official Symbol
DRAM1
NCBI Official Synonym Symbols
DRAM
UniProt Protein Name
DNA damage-regulated autophagy modulator protein 1
UniProt Synonym Protein Names
Damage-regulated autophagy modulator
UniProt Gene Name
DRAM1
UniProt Synonym Gene Names
DRAM
UniProt Entry Name
DRAM1_HUMAN
NCBI Protein Information
DNA damage-regulated autophagy modulator protein 1

NCBI Summary for DRAM1

This gene is regulated as part of the p53 tumor suppressor pathway. The gene encodes a lysosomal membrane protein that is required for the induction of autophagy by the pathway. Decreased transcriptional expression of this gene is associated with various tumors. This gene has a pseudogene on chromosome 4. [provided by RefSeq, Jul 2008]
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UniProt Comments for DRAM1

Function: Lysosomal modulator of autophagy that plays a central role in p53/TP53-mediated apoptosis. Not involved in p73/TP73-mediated autophagy. Ref.3 Ref.4

Subcellular location: Lysosome membrane; Multi-pass membrane protein Ref.3.

Induction: By p53/TP53 and p73/TP73. Directly activated by p53/TP53. Significantly down-regulated in tumor cell lines by methylation-dependent transcriptional silencing. Ref.3 Ref.4

Sequence similarities: Belongs to the DRAM/TMEM150 family.

Sequence caution: The sequence BAA92091.1 differs from that shown. Reason: Erroneous initiation.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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