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TREF1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649623

TREF1 encodes a zinc-finger transcriptional regulating protein which interacts with CBP/p300 to regulate the human gene CYP11A1. More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TREF1, Polyclonal Antibody
Full Product Name
TREF1, CT (TRERF1, BCAR2, RAPA, TREP132, Transcriptional-regulating factor 1, Breast cancer anti-estrogen resistance 2, Transcriptional-regulating protein 132, Zinc finger protein rapa, Zinc finger transcription factor TReP-132)
Product Synonym Names
Anti -TREF1, CT (TRERF1, BCAR2, RAPA, TREP132, Transcriptional-regulating factor 1, Breast cancer anti-estrogen resistance 2, Transcriptional-regulating protein 132, Zinc finger protein rapa, Zinc finger transcription factor TReP-132)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
TREF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1143~1172 amino acids from the C-terminal region of human TREF1.

Product Categories/Family for anti-TREF1 antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger
Applications Tested/Suitable for anti-TREF1 antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-TREF1 antibody
Suitable for use in Western Blot, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-TREF1 antibody

Western Blot analysis in K562 cell line lysates (35ug/lane) using MBS649623. TREF1 (arrow) was detected using MBS649623.
Rabbit Anti-TREF1 Polyclonal Antibody – Cat# MBS649623

Immunofluorescence (IF) of anti-TREF1 antibody

Fluorescent image of MCF-7 cell stained with MBS649623. MCF-7 cells were fixed with 4% PFA (20mins), permeabilized with Triton X-100 (0.1%, 10mins), then incubated with MBS649623 (1:25, 1hr at 37ºC). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50mins at 37ºC).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37ºC).TREF1 immunoreactivity is localized to Nucleus significantly.
Rabbit Anti-TREF1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS649623

Flow Cytometry (FC/FACS) of anti-TREF1 antibody

Flow Cytometric analysis of K562 cells MBS649623 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-TREF1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS649623

Other Notes

Small volumes of anti-TREF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TREF1 purchase

MBS649623 is an antibody targeting TREF1. Also known as TREF1, CT (TRERF1, BCAR2, RAPA, TREP132, Transcriptional-regulating factor 1, Breast cancer anti-estrogen resistance 2, Transcriptional-regulating protein 132, Zinc finger protein rapa, Zinc finger transcription factor TReP-132). Reactive in Human. Host: Rabbit.
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