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IL32 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649655

This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after… More details

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Product Overview

Online Datasheet in PDF
Product Name
IL32, Polyclonal Antibody
Full Product Name
IL32, NT (IL32, NK4, TAIF, Interleukin-32, Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor)
Product Gene Name
Product Synonym Names
Anti -IL32, NT (IL32, NK4, TAIF, Interleukin-32, Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
26,676 Da
Chromosome Location
Chromosome: 16; NC_000016.9 (3115313..3119668). Location: 16p13.3
OMIM
606001
3D Structure
ModBase 3D Structure for P24001
Immunogen
IL32 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-53 amino acids from the N-terminal region of human IL32.
Applications Tested/Suitable for anti-IL32 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-IL32 antibody
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50

Western Blot (WB) of anti-IL32 antibody

Western Blot analysis in SK-BR-3 cell line lysates (35ug/lane) using MBS649655. This demonstrates that MBS649655 detected the IL32 protein (arrow).
Rabbit Anti-IL32 Polyclonal Antibody – Cat# MBS649655

Immunohistochemistry (IHC) of anti-IL32 antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue using MBS649655 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS649655 for immunohistochemistry.
Rabbit Anti-IL32 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS649655

Other Notes

Small volumes of anti-IL32 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL32 purchase

MBS649655 is an antibody targeting IL32. Also known as IL32, NT (IL32, NK4, TAIF, Interleukin-32, Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IL32. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
interleukin-32 isoform B
NCBI Official Synonym Full Names
interleukin 32
NCBI Official Symbol
IL32
NCBI Official Synonym Symbols
NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma
UniProt Protein Name
Interleukin-32
UniProt Synonym Protein Names
Natural killer cells protein 4; Tumor necrosis factor alpha-inducing factor
Protein Family
UniProt Gene Name
IL32
UniProt Synonym Gene Names
NK4; TAIF; IL-32
UniProt Entry Name
IL32_HUMAN
NCBI Protein Information
interleukin-32; interleukin-32 eta; interleukin-32 small; interleukin-32 theta; natural killer cells protein 4; natural killer cell transcript 4; tumor necrosis factor alpha-inducing factor

UniProt Comments for IL32

Function: Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK. Ref.2

Subcellular location: Secreted Ref.2.

Tissue specificity: Selectively expressed in lymphocytes. Expression is more prominent in immune cells than in non-immune cells. Ref.2

Induction: Expression increased after activation of T-cells by mitogens or activation of NK cells by IL2/interleukin-2. Ref.1
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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