Rabbit Anti-ARHGAP24 Polyclonal Antibody – Cat# MBS649678

Western Blot analysis in SK-BR-3 cell line lysates (35ug/lane) using MBS649678. This demonstrates MBS649678 detected the ARHGAP24 protein (arrow).

ARHGAP24 Antibody – Rabbit Polyclonal

Name: ARHGAP24 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS649678
Price: 895 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649678

ARHGAPs, such as ARHGAP24, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]). More details

$895 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

ARHGAP24, Polyclonal Antibody

Full Product Name

ARHGAP24, CT (ARHGAP24, FILGAP, Rho GTPase-activating protein 24, Filamin-A-associated RhoGAP, RAC1- and CDC42-specific GTPase-activating protein of 72kD, Rho-type GTPase-activating protein 24, RhoGAP of 73kD, Sarcoma antigen NY-SAR-88, p73RhoGAP)

Product Gene Name

anti-ARHGAP24 antibody

Product Synonym Names

Anti -ARHGAP24, CT (ARHGAP24, FILGAP, Rho GTPase-activating protein 24, Filamin-A-associated RhoGAP, RAC1- and CDC42-specific GTPase-activating protein of 72kD, Rho-type GTPase-activating protein 24, RhoGAP of 73kD, Sarcoma antigen NY-SAR-88, p73RhoGAP)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Human

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

84,258 Da

Chromosome Location

Chromosome: 4; NC_000004.11 (86068860..86923823). Location: 4q22.1

OMIM

610586

3D Structure

ModBase 3D Structure for Q8N264

Immunogen

ARHGAP24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 586-616 amino acids from the C-terminal region of human ARHGAP24.

Product Categories/Family for anti-ARHGAP24 antibody

Antibodies; Abs to Cell Division Cycle (CDC)

Applications Tested/Suitable for anti-ARHGAP24 antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-ARHGAP24 antibody

Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500

Other Notes

Small volumes of anti-ARHGAP24 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ARHGAP24 purchase

MBS649678 is an antibody targeting ARHGAP24. Also known as ARHGAP24, CT (ARHGAP24, FILGAP, Rho GTPase-activating protein 24, Filamin-A-associated RhoGAP, RAC1- and CDC42-specific GTPase-activating protein of 72kD, Rho-type GTPase-activating protein 24, RhoGAP of 73kD, Sarcoma antigen NY-SAR-88, p73RhoGAP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ARHGAP24. It may not necessarily be applicable to this product.

NCBI GI #

28839072

NCBI GeneID

83478

NCBI Accession #

AAH47918.1

UniProt Primary Accession #

Q8N264

UniProt Secondary Accession #

Q4KMG1; Q6ZNV3; Q86TI5; Q86WE4; Q9H0T6

UniProt Related Accession #

Q8N264

NCBI Official Full Name

ARHGAP24 protein

NCBI Official Synonym Full Names

Rho GTPase activating protein 24

NCBI Official Symbol

ARHGAP24

NCBI Official Synonym Symbols

p73; FILGAP; RCGAP72; RC-GAP72; p73RhoGAP

UniProt Protein Name

Rho GTPase-activating protein 24

UniProt Synonym Protein Names

Filamin-A-associated RhoGAP; FilGAP; RAC1- and CDC42-specific GTPase-activating protein of 72 kDa; RC-GAP72; Rho-type GTPase-activating protein 24; RhoGAP of 73 kDa; Sarcoma antigen NY-SAR-88; p73RhoGAP

Protein Family

Rho GTPase-activating protein

UniProt Gene Name

ARHGAP24

UniProt Synonym Gene Names

FILGAP; FilGAP; RC-GAP72

UniProt Entry Name

RHG24_HUMAN

NCBI Protein Information

rho GTPase-activating protein 24; rhoGAP of 73 kDa; sarcoma antigen NY-SAR-88; filamin-A-associated RhoGAP; rho-type GTPase-activating protein 24; RAC1- and CDC42-specific GTPase-activating protein of 72 kDa

UniProt Comments for ARHGAP24

Function: Rho GTPase-activating protein involved in cell polarity, cell morphology and cytoskeletal organization. Acts as a GTPase activator for the Rac-type GTPase by converting it to an inactive GDP-bound state. Controls actin remodeling by inactivating Rac downstream of Rho leading to suppress leading edge protrusion and promotes cell retraction to achieve cellular polarity. Able to suppress RAC1 and CDC42 activity in vitro. Overexpression induces cell rounding with partial or complete disruption of actin stress fibers and formation of membrane ruffles, lamellipodia, and filopodia. Isoform 2 is a vascular cell-specific GAP involved in modulation of angiogenesis. Ref.6 Ref.7 Ref.8

Subunit structure: Interacts with FLNA. Ref.8

Subcellular location: Cytoplasm › cytoskeleton. Cell junction › adherens junction. Cell junction › focal adhesion. Cell projection. Note: Localizes to actin stress fibers. In migrating cells, localizes to membrane lamellae and protusions. Ref.7 Ref.8

Tissue specificity: Isoform 1 is widely expressed with a higher level in kidney. Isoform 2 is mainly expressed in endothelial cells. Ref.6 Ref.7 Ref.8

Induction: Isoform 2 is up-regulated during capillary tube formation in umbilical vein endothelial cells. Ref.6

Domain: The coiled coil domain mediates the interaction with FLNA leading to its recruitment to lamellae.

Post-translational modification: Phosphorylated by ROCK, leading to activate the RacGAP activity. Ref.8

Sequence similarities: Contains 1 PH domain.Contains 1 Rho-GAP domain.

Sequence caution: The sequence AAO65178.1 differs from that shown. Reason: Frameshift at several positions.

Research Articles on ARHGAP24

1. Data indicate that phosphorylation of FilGAP by ROCK appears to promote amoeboid morphology.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ARHGAP24 antibody

Reference Product	PubMed Publications
FLNA antibody	>6 publications with ARHGAP24 and FLNA
CDC42 antibody	>4 publications with ARHGAP24 and CDC42
RAC1 antibody	>2 publications with ARHGAP24 and RAC1
SCN10A antibody	>1 publications with ARHGAP24 and SCN10A

Pathways associated with anti-ARHGAP24 antibody

Products by Pathway	Pathway Diagram
Rho GTPase Cycle Pathway antibodies	Rho GTPase Cycle Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By Rho GTPases Pathway antibodies	Signaling By Rho GTPases Pathway Diagram

Diseases associated with anti-ARHGAP24 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>2 publications with ARHGAP24 and Neoplasms
Poisoning Antibodies	>1 publications with ARHGAP24 and Poisoning
Autoimmune Diseases Antibodies	>1 publications with ARHGAP24 and Autoimmune Diseases
Carcinoma Antibodies	>1 publications with ARHGAP24 and Carcinoma
Kidney Diseases Antibodies	>1 publications with ARHGAP24 and Kidney Diseases
Liver Diseases Antibodies	>1 publications with ARHGAP24 and Liver Diseases
Breast Neoplasms Antibodies	>1 publications with ARHGAP24 and Breast Neoplasms
Drug-Induced Liver Injury Antibodies	>1 publications with ARHGAP24 and Drug-Induced Liver Injury

Organs/Tissues associated with anti-ARHGAP24 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>2 publications with ARHGAP24 and Brain
Kidney Antibodies	>2 publications with ARHGAP24 and Kidney
Heart Antibodies	>1 publications with ARHGAP24 and Heart
Liver Antibodies	>1 publications with ARHGAP24 and Liver
Lung Antibodies	>1 publications with ARHGAP24 and Lung
Blood Antibodies	>1 publications with ARHGAP24 and Blood
Muscle Antibodies	>1 publications with ARHGAP24 and Muscle
Uterus Antibodies	>1 publications with ARHGAP24 and Uterus