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VILL Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649681

The protein encoded by this gene belongs to the villin/gelsolin family. It contains 6 gelsolin-like repeats and a headpiece domain. It may play a role in actin-bundling. [provided by RefSeq]. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
VILL, Polyclonal Antibody
Full Product Name
VILL, ID (VILL, Villin-like protein)
Product Gene Name
Product Synonym Names
Anti -VILL, ID (VILL, Villin-like protein)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
95,907 Da
Chromosome Location
Chromosome: 3; NC_000003.11 (38032217..38048676). Location: 3p21.3
3D Structure
ModBase 3D Structure for O15195
Immunogen
VILL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 392-420 amino acids from the Central region of human VILL.

Product Categories/Family for anti-VILL antibody

Applications Tested/Suitable for anti-VILL antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-VILL antibody
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Immunofluorescence: 1:10-50
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-VILL antibody

Western Blot analysis in K562 cell line lysates (35ug/lane) using MBS649681. This demonstrates that MBS649681 detected the VILL protein (arrow).
Rabbit Anti-VILL Polyclonal Antibody – Cat# MBS649681

Immunohistochemistry (IHC) of anti-VILL antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle using MBS649681 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS649681 for immunohistochemistry.
Rabbit Anti-VILL Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS649681

Immunofluorescence (IF) of anti-VILL antibody

Confocal immunofluorescent analysis with WiDr cell using MBS649681followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-VILL Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS649681

Flow Cytometry (FC/FACS) of anti-VILL antibody

Flow Cytometry analysis of K562 cells using MBS649681 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-VILL Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS649681

Other Notes

Small volumes of anti-VILL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VILL purchase

MBS649681 is an antibody targeting VILL. Also known as VILL, ID (VILL, Villin-like protein). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for VILL. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
VILL protein
NCBI Official Synonym Full Names
villin-like
NCBI Official Symbol
VILL
UniProt Protein Name
Villin-like protein
Protein Family
UniProt Gene Name
VILL
UniProt Entry Name
VILL_HUMAN
NCBI Protein Information
villin-like protein

NCBI Summary for VILL

The protein encoded by this gene belongs to the villin/gelsolin family. It contains 6 gelsolin-like repeats and a headpiece domain. It may play a role in actin-bundling. [provided by RefSeq, Jul 2008]

UniProt Comments for VILL

Function: Possible tumor suppressor.

Tissue specificity: Ubiquitously expressed in 16 tissues examined.

Sequence similarities: Belongs to the villin/gelsolin family.Contains 6 gelsolin-like repeats.Contains 1 HP (headpiece) domain.

Sequence caution: The sequence AAH00243.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH04300.1 differs from that shown. Reason: Erroneous initiation.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-VILL antibody

 Reference Product  PubMed Publications
 CTDSPL antibody  >2 publications with VILL and CTDSPL
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