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REST Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6497271

MBS6497271 is an antibody targeting REST. Also known as REST (Repressor Element RE-1 Silencing Transcription Factor, NRSF, Neuronal Restricted Silencing Factor) (HRP). Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$1170 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
REST, Polyclonal Antibody
Full Product Name
REST (Repressor Element RE-1 Silencing Transcription Factor, NRSF, Neuronal Restricted Silencing Factor) (HRP)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human REST, Mr 120-200kD. Species Crossreactivity: mouse and rat
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Molecular Weight
1097
Chromosome Location
Chromosome: 4; NC_000004.11 (57774042..57802010). Location: 4q12
OMIM
600571
3D Structure
ModBase 3D Structure for Q13127
Immunogen
GST fusion protein corresponding to residues 801-1097 of human REST (NM_005612).
Conjugate
HRP
Note
Preservative Free
Applications Tested/Suitable for anti-REST antibody
Western Blot (WB)
Application Notes for anti-REST antibody
Applications are based on unconjugated antibody.
WB: 1:500-1:2000. ES cell total extract was resolved by electrophoresis, transferred to nitrocellulose and probed with. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.

Western Blot (WB) of anti-REST antibody

Figure: WB: 20ug of ES Cell total extract was probed with (0.5ug/ml).
Rabbit Anti-REST Polyclonal Antibody – Cat# MBS6497271

Product References and Citations for anti-REST antibody

1. Ballas, N., et.al., Neuron 31: 353-365, 2001. 2. Schoenherr, C.J., et.al., Science 267: 1360-1363, 1995. 3. Chong, J.A., et.al., Cell 80: 949-957, 1995.

Other Notes

Small volumes of anti-REST antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for REST. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
RE1-silencing transcription factor
NCBI Official Synonym Full Names
RE1-silencing transcription factor
NCBI Official Symbol
REST
NCBI Official Synonym Symbols
XBR; NRSF
UniProt Protein Name
RE1-silencing transcription factor
UniProt Synonym Protein Names
Neural-restrictive silencer factor; X2 box repressor
UniProt Gene Name
REST
UniProt Synonym Gene Names
NRSF; XBR
UniProt Entry Name
REST_HUMAN
NCBI Protein Information
RE1-silencing transcription factor; repressor binding to the X2 box; neural-restrictive silencer factor; neuron restrictive silencer factor

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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